Obesity Clinical Trial
Official title:
Prestudy: Lifestyle and Cardiovascular Disease
The purpose of this pilot study is to optimize conditions for a planned human intervention study focusing on how diet predispose for, and influence, lifestyle disease development and its consequence in cardiovascular disease development.
Common for many of the risk factors of lifestyle diseases is that they are induced by
improper diet. Recent research has shown that especially total amount and composition of the
macro nutrients, protein, carbohydrate and fats, is important.
A time course experiment has been performed where 5 subjects underwent a diet intervention
for four weeks with a controlled, balanced macro nutrient energy content of every meal. The
sampling time points were 0, 1, 2, 7, 14 and 28 days. The goals were to determine optimal
length of intervention with stabilization of gene expression to occur and compare blood and
subcutaneous abdominal fat tissue as source of biological material for RNA for DNA
microarray analysis. Microarray analysis were performed on 3 of the 5 subjects.
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