Obesity-related Genes in Taiwanese Undergoing Weight Loss

Morbid Obesity Clinical Trial

Official title:

Searching for the Most Influential Obesity-related Diabetic Genes and Exploring the Expression Profiles of Those Genes in Taiwanese Undergoing Weight Loss

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT01684280
• Other study ID #: CRC-06-09-10
• Status: Completed
• Phase: N/A
• First received: September 10, 2012
• Last updated: September 11, 2012
• Start date: June 2010
• Est. completion date: September 2010

Study information

• Verified date: September 2012
• Source: Taipei Medical University Hospital
• Contact: n/a
• Is FDA regulated: No
• Health authority: Taiwan: Department of Health
• Study type: Observational

Clinical Trial Summary

To investigate:

1. Associations between miRNA and insulin signaling-related gene expressions in abdominal adipose tissues in obese subjects.

2. Differences in miRNAs expressed by intrabdominal omental adipose tissues between genders.

Description:

Compared the differences in microRNA expressions of abdominal adipose tissue in morbid obese male and female subjects to elucidate the possible regulatory role of microRNA in insulin signaling-related gene expression levels and its association with biochemical markers. We hope to find a miRNA that can be used to predict the syndrome associated with obesity.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 20
• Est. completion date: September 2010
• Est. primary completion date: September 2010
• Accepts healthy volunteers: No
• Gender: Both
• Age group: 19 Years to 63 Years

Eligibility

Inclusion Criteria:

• Morbid obesity,BMIs> 40 kg/m2

• Subjects who underwent bariatric surgery

Exclusion Criteria:

• Obese subjects with complicated metabolic disorders

• Thyroid disease

Study Design

Observational Model: Case Control, Time Perspective: Cross-Sectional

Related Conditions & MeSH terms

• Morbid Obesity
• Obesity
• Obesity, Morbid

Intervention

Genetic:
• gender
Paired samples of abdominal subcutaneous and intrabdominal omental adipose tissue were obtained from men and women who underwent bariatric surgery.

Locations

Country	Name	City	State
Taiwan	Taipei Medical University Hospital	Taipei

Sponsors (2)

Lead Sponsor	Collaborator
Sung Ling Yeh	National Science Council, Taiwan

Country where clinical trial is conducted

Taiwan, 

References & Publications (12)

Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell. 2004 Jan 23;116(2):281-97. Review. — View Citation

Brennecke J, Stark A, Russell RB, Cohen SM. Principles of microRNA-target recognition. PLoS Biol. 2005 Mar;3(3):e85. — View Citation

Gustafson B. Adipose tissue, inflammation and atherosclerosis. J Atheroscler Thromb. 2010 Apr 30;17(4):332-41. Epub 2010 Feb 3. Review. — View Citation

Herrera BM, Lockstone HE, Taylor JM, Wills QF, Kaisaki PJ, Barrett A, Camps C, Fernandez C, Ragoussis J, Gauguier D, McCarthy MI, Lindgren CM. MicroRNA-125a is over-expressed in insulin target tissues in a spontaneous rat model of Type 2 Diabetes. BMC Med — View Citation

Hilton C, Neville MJ, Karpe F. MicroRNAs in adipose tissue: their role in adipogenesis and obesity. Int J Obes (Lond). 2013 Mar;37(3):325-32. doi: 10.1038/ijo.2012.59. Epub 2012 Apr 24. Review. — View Citation

Jiang L, Huang Q, Zhang S, Zhang Q, Chang J, Qiu X, Wang E. Hsa-miR-125a-3p and hsa-miR-125a-5p are downregulated in non-small cell lung cancer and have inverse effects on invasion and migration of lung cancer cells. BMC Cancer. 2010 Jun 22;10:318. doi: 1 — View Citation

John B, Enright AJ, Aravin A, Tuschl T, Sander C, Marks DS. Human MicroRNA targets. PLoS Biol. 2004 Nov;2(11):e363. Epub 2004 Oct 5. Erratum in: PLoS Biol. 2005 Jul;3(7):e264. — View Citation

Kanehisa M, Goto S. KEGG: kyoto encyclopedia of genes and genomes. Nucleic Acids Res. 2000 Jan 1;28(1):27-30. — View Citation

Krotkiewski M, Björntorp P, Sjöström L, Smith U. Impact of obesity on metabolism in men and women. Importance of regional adipose tissue distribution. J Clin Invest. 1983 Sep;72(3):1150-62. — View Citation

Niswender KD, Morrison CD, Clegg DJ, Olson R, Baskin DG, Myers MG Jr, Seeley RJ, Schwartz MW. Insulin activation of phosphatidylinositol 3-kinase in the hypothalamic arcuate nucleus: a key mediator of insulin-induced anorexia. Diabetes. 2003 Feb;52(2):227 — View Citation

O'Connell J, Lynch L, Cawood TJ, Kwasnik A, Nolan N, Geoghegan J, McCormick A, O'Farrelly C, O'Shea D. The relationship of omental and subcutaneous adipocyte size to metabolic disease in severe obesity. PLoS One. 2010 Apr 1;5(4):e9997. doi: 10.1371/journa — View Citation

Wellen KE, Hotamisligil GS. Inflammation, stress, and diabetes. J Clin Invest. 2005 May;115(5):1111-9. Review. — View Citation

* Note: There are 12 references in all — Click here to view all references

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	MicroRNA expression in abdominal omental adipose tissues	MicroRNA-125a-3p expression in abdominal omental adipose tissues is associated with insulin signaling-related gene expression levels in morbid obesity	up to 28 weeks	Yes

External Links

•   Comprehensive Weight Management Center Taipei Medical University Hospital
•   Taipei Medical University-Joint Institutional Review Board