Reversal of the Anti-platelet Effects of Ticagrelor

Coronary Artery Disease Clinical Trial

— REVERSAL  

Official title:

Reversal of the Anti-platelet Effects of Ticagrelor in Healthy Persons and Patients With Coronary Artery Disease

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT02383771
• Other study ID #: 003
• Status: Completed
• Phase: Phase 4
• First received: February 5, 2015
• Last updated: August 14, 2017
• Start date: March 2015
• Est. completion date: December 2016

Study information

• Verified date: August 2017
• Source: The First Affiliated Hospital with Nanjing Medical University
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The purpose of this study is to determine the proportion of untreated donor platelets required to fully reverse the platelet inhibitory effects of ticagrelor and aspirin in healthy persons and patients with coronary artery disease undergoing percutaneous coronary intervention (PCI) and receiving ticagrelor.

Description:

Reversal of the Anti-platelet Effects of Ticagrelor: REVERSAL study

The fatality of stent thrombosis (ST) in patients with coronary artery disease (CAD) undergoing percutaneous coronary intervention (PCI) is approximately 50% and clopidogrel is an important anti-platelet drug for prevention of ST. CAD patients implanted with stent including bare metal stent (BMS) and drug eluting stent (DES) are recommended to receive dual anti-platelet treatment (DAPT), i.e. clopidogrel along with aspirin, for at least one year to reduce the incidence of ST by up-to-date guidelines. However, due to the variability of anti-platelet effect of clopidogrel, regular dose (75 mg daily) of clopidogrel administered cannot achieve enough inhibition of platelet aggregation in 20-30% of total patients, which is named as clopidogrel low responsiveness (CLR), and the morbidity of thrombosis (including) in CAD patients is still 10%.

Ticagrelor, a cyclopentyl-triazolo-pyrimidine, is a more potent adenosine diphosphate (ADP) receptor antagonist with faster onset and more significantly higher inhibition of platelet aggregation compared with clopidogrel and directly acts on P2Y12-ADP receptor in platelets without process of hepatic metabolism. In the PLATO study, ticagrelor plus reduced the remarkable incidence of cardiovascular events in patients with acute coronary syndrome (ACS) without significant higher incidence of major bleeding events compared with clopidogrel plus aspirin. Surprisingly, the incidence of death due to cardiovascular causes and the total fatality was decreased in patients with ticagrelor plus aspirin compared with those with clopidogrel plus aspirin. The results suggested the more benefit brought by ticagrelor, highlighting the wide use of it in the future.

Due to the potent anti-platelet effect of ticagrelor, more bleeding events may occur. Additionally, when facing the need for cardiac or non-cardiac operation, occurrence of life-threatening bleeding event or necessity of emergency operation, doctors may be confused of the treatment for the patients taking ticagrelor, of which the half-life period is 8-9 hours and it suggests the importance of studying the reversal of the anti-platelet effects of ticagrelor.

The primary objective of this study is to investigate the proportion of untreated donor platelets required to fully reverse the platelet inhibitory effects of aspirin and ticagrelor in healthy persons and patients with coronary artery disease undergoing percutaneous coronary intervention (PCI) and receiving ticagrelor.

Study Population:

The investigators design two cohort studies, and plan to enroll 32 healthy volunteers in cohort 1 and 16 patients with coronary artery disease undergoing percutaneous coronary intervention (PCI) and receiving dual anti-platelet therapy (ticagrelor 90mg bid + aspirin 100mg daily) for 7 days

Cohort 1:

Randomization:

A total of 32 healthy volunteers are planned to be enrolled and will be randomly divided into three groups: single anti-platelet treatment group (A group, 8 of 32), dual anti-platelet treatment group (B group, 8 of 32) and control group (C group, 16 of 32).

1. Single anti-platelet treatment group: (Ticagrelor 90mg bid) × 7 days

2. Dual anti-platelet treatment group: (Ticagrelor 90mg bid + Aspirin 100mg daily) × 7 days

3. Control group: No anti-platelet therapy

Inclusion criteria:

1. Healthy volunteers

2. Participants aged >18 years old

Exclusion criteria:

1. Allergy or intolerance to aspirin or ticagrelor;

2. Subjects at a high risk of bleeding (e.g. platelet count<100×10^9/L, history of peptic ulcer, hemoglobin<110g/L);

3. Subjects with bronchial asthma or chronic obstructive pulmonary disease;

4. Subjects with bradycardia (e.g. sick sinus syndrome, high-grade atrioventricular block, history of syncope with unproved uncorrelation with bradycardia);

5. Smokers;

6. Subjects with diabetes mellitus;

7. Subjects planning to be pregnancy;

8. Subjects with hepatic or renal dysfunction;

9. Subjects who have taken other anti-platelet drugs, non-steroidal anti-inflammatory drugs or proton pump inhibitors before scanning or need to take them during study period.

Blood collection and sample preparation

Venous blood samples are collected by venipuncture into two 4.5-mL draw BD vacutainer tubes containing 0.105M buffered sodium citrate (3.2%) at 08.00 hours in the morning before taking the agent and after the last dose of the study drug. Platelet-rich plasma (PRP) from subjects in A group is mixed with increasing proportions of that in C group, with one untreated subject serving as the control for one treated subject. The proportion of control platelets mixed with inhibited platelets is calculated based on platelet numbers, starting at 10% and increasing by 10% increments. So as the PRP in B group.

Test:

1. Before taking the agent:

1. ADP-induced platelet aggregation: light transmittance aggregation (LTA) in response to 5μM ADP

2. Arachidonic acid (AA)-induced platelet aggregation: LTA in response to 1mM AA

2. After 7-day medication:

1. ADP-induced platelet aggregation: LTA in response to 5μM ADP

2. AA-induced platelet aggregation: LTA in response to 1mM AA

3. ADP-induced platelet aggregation of mixed sample: LTA in response to 5μM ADP

4. AA-induced platelet aggregation of mixed sample: LTA in response to 1mM AA

Primary end points:

1. Reversal of the platelet inhibitory effects of antiplatelet therapy Proportion of untreated donor platelets required to fully reverse the platelet inhibitory effects of antiplatelet therapy in healthy volunteers

Secondary end points:

1. Inhibition of platelet aggregation (IPA) in response to 5μM ADP determined by light transmittance aggregometer (LTA) after 7-day ticagrelor administration

Safety issue: No

Cohort 2:

A total of 16 patients with diagnosed coronary artery disease who have undergone percutaneous coronary intervention (PCI) and have received dual anti-platelet therapy (ticagrelor 90mg bid + aspirin 100mg daily) for 7 days is planned to be enrolled.

Inclusion Criteria:

1. Subjects with diagnosed coronary artery disease undergoing percutaneous coronary intervention (PCI);

2. Subjects who have received dual anti-platelet therapy (ticagrelor 90mg bid + aspirin 100mg daily) for 7 days;

Exclusion Criteria:

1. Subjects at a high risk of bleeding (e.g. platelet count<100×10^9/L, history of peptic ulcer, hemoglobin<110g/L);

2. Subjects with anemia;

3. Smokers

4. Subjects planning to be pregnancy;

5. Subjects with hepatic or renal dysfunction;

6. Subjects who have taken other anti-platelet drugs, non-steroidal anti-inflammatory drugs or proton pump inhibitors before scanning or need to take them during study period.

Sample test for fresh platelet saved in blood bank

Function of platelet aggregation, platelet count, pH value and metabolic products (including PO2, PCO2, blood glucose, lactate, bicarbonate, sodium, potassium and chloride) are measured in fresh platelet sample preserved in blood bank for 1, 2, 3, 4, and 5 days respectively.

Blood collection and sample preparation for patients

Venous blood samples are collected by venipuncture into two 4.5-mL draw BD vacutainer tubes containing 0.105M buffered sodium citrate (3.2%) at 08.00 hours in the morning after taking the medicine. Platelet-rich plasma (PRP) from participants is mixed with increasing proportions of that extracted from fresh platelet sample reserved in blood bank for one day as the control for each treated subject. The proportion of control platelets mixed with inhibited platelets is calculated based on platelet numbers, starting at 10% and increasing by 10% increments. So as the PRP mixed with fresh platelet sample preserved for four days.

Test for sample from patients

1. Before mixture:

1. ADP-induced platelet aggregation: light transmittance aggregation (LTA) in response to 5μM ADP

2. Arachidonic acid (AA)-induced platelet aggregation: LTA in response to 1mM AA

2. After mixture:

1. ADP-induced platelet aggregation: LTA in response to 5μM ADP

2. AA-induced platelet aggregation: LTA in response to 1mM AA

Primary end points:

1. Reversal of the platelet inhibitory effects of antiplatelet therapy Proportion of untreated donor platelets required to fully reverse the platelet inhibitory effects of antiplatelet therapy in patients with coronary artery disease undergoing percutaneous coronary intervention (PCI) and receiving ticagrelor

Secondary end points:

1. Relationship between function of platelet aggregation and the time of saving fresh platelet.

Safety issue: No

Recruitment information / eligibility

• Status: Completed
• Enrollment: 64
• Est. completion date: December 2016
• Est. primary completion date: November 2016
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 18 Years to 80 Years

Eligibility

Cohort 1:

Inclusion Criteria:

• Healthy volunteers;

• Subjects aged >18 years old;

Exclusion Criteria:

• Allergy or intolerance to aspirin or ticagrelor;

• Subjects at a high risk of bleeding (e.g. platelet count<100×10^9/L, history of peptic ulcer, hemoglobin<110g/L);

• Subjects with anemia;

• Subjects with bronchial asthma or chronic obstructive pulmonary disease;

• Subjects with bradycardia (e.g. sick sinus syndrome, high-grade atrioventricular block, history of syncope with unproved uncorrelation with bradycardia);

• Smokers;

• Subjects with diabetes mellitus;

• Subjects planning to be pregnancy;

• Subjects with hepatic or renal dysfunction;

• Subjects who have taken other anti-platelet drugs, non-steroidal anti-inflammatory drugs or proton pump inhibitors before scanning or need to take them during study period.

Cohort 2:

Inclusion Criteria:

• Subjects with diagnosed coronary artery disease undergoing percutaneous coronary intervention (PCI);

• Subjects who have received dual anti-platelet therapy (ticagrelor 90mg bid + aspirin 100mg daily) for 7 days;

Exclusion Criteria:

• Subjects at a high risk of bleeding (e.g. platelet count<100×10^9/L, history of peptic ulcer, hemoglobin<110g/L);

• Subjects with anemia;

• Smokers

• Subjects planning to be pregnancy;

• Subjects with hepatic or renal dysfunction;

• Subjects who have taken other anti-platelet drugs, non-steroidal anti-inflammatory drugs or proton pump inhibitors before scanning or need to take them during study period.

Related Conditions & MeSH terms

• Coronary Artery Disease
• Coronary Disease
• Myocardial Ischemia

Intervention

Drug:
• Ticagrelor
(Ticagrelor 90mg Bid) x 7days
• Aspirin + Ticagrelor
(Aspirin 100mg daily + Ticagrelor 90mg Bid) x 7days
• Control
No Anti-platelet Therapy
• Aspirin + Ticagrelor
Dual anti-platelet therapy (Aspirin 100mg daily + Ticagrelor 90mg Bid) after percutaneous coronary intervention (PCI)

Locations

Country	Name	City	State
China	First Affiliated Hospital of Nanjing Medical University	Nanjing	Jiangsu

Sponsors (2)

Lead Sponsor	Collaborator
The First Affiliated Hospital with Nanjing Medical University	National Natural Science Foundation of China

Country where clinical trial is conducted

China, 

References & Publications (17)

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* Note: There are 17 references in all — Click here to view all references

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Reversal of the platelet inhibitory effects of antiplatelet therapy in healthy volunteers	Proportion of untreated donor platelets required to fully reverse the platelet inhibitory effects of antiplatelet therapy	7 days after randomization
Primary	Reversal of the platelet inhibitory effects of antiplatelet therapy in patients	Reversal of the platelet inhibitory effects of antiplatelet therapy Proportion of untreated donor platelets required to fully reverse the platelet inhibitory effects of antiplatelet therapy in patients with coronary artery disease undergoing percutaneous coronary intervention (PCI) and receiving ticagrelor	7 days after percutaneous coronary intervention
Secondary	Inhibition of platelet aggregation in response to AA or ADP	Inhibition of platelet aggregation (IPA) in response to 5µM ADP determined by light transmittance aggregometer (LTA) after 7-day ticagrelor administration	7 days after randomization
Secondary	Change of ADP-induced platelet aggregation in platelets saved in blood bank due to the saving time	How the ADP-induced platelet aggregation in platelets saved in blood bank changes due to the saving time	5 days after fresh platelet collected and stored in blood bank