Expandability of Aging Adipose Tissue

Obesity Clinical Trial

— EAT  

Official title:

Aging Subcutaneous Adipose Tissue Response to Overfeeding

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT02456870
• Other study ID #: 14-2411
• Status: Completed
• Phase: N/A
• Start date: June 2015
• Est. completion date: December 2018

Study information

• Verified date: June 2019
• Source: University of Colorado, Denver
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The purpose of this pilot study is to determine whether adipose tissue loses some of its ability to expand in response to overfeeding in older compared to younger men. The investigators hypothesize that the adipose tissue response to overfeeding may be weakened in older when compared to younger men, thereby leading to worsened ectopic storage depots for older men.

Description:

This pilot study will begin to address whether adipose tissue biology changes with advancing age. Specifically, we will test whether adipose tissue loses some of its ability to expand in response to overfeeding in older compared to younger men. To test this possibility the Investigators will recruit overweight and mildly obese older (age 55-75y) and younger (age 25-40y) men and collect adipose tissue biopsies before and after 7 days of overfeeding. The investigators will test how well the cells in adipose tissue expand in response to overfeeding and whether this differs between younger and older men.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 20
• Est. completion date: December 2018
• Est. primary completion date: December 2016
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Male
• Age group: 25 Years to 75 Years

Eligibility

Inclusion Criteria:

• Men

• Aged 25-40y OR 55-75y

• BMI 28-35 kg/m2

• Weight stable (±2kg over past 3 mo)

• Sedentary to moderately active (<3 d/wk of exercise lasting >20min)

• Non-smokers (never used or quit >7yrs prior)

Exclusion Criteria:

• Women

• Currently using hormones, glucose or lipid lowering medication

• Engaged in exercise or diet program

• Currently gaining or losing weight or using weight loss drugs

• Diabetes mellitus (diagnosis, treatment, FBG =126 mg/dL)

• Uncontrolled metabolic disorders (e.g., thyroid)

Related Conditions & MeSH terms

• Aging
• Obesity

Intervention

Other:
• Overfeeding
Consumption of 40% excess calories for 7 consecutive days

Locations

Country	Name	City	State
United States	Univeristy of Colorado, Anschutz Medical Campus	Aurora	Colorado

Sponsors (2)

Lead Sponsor	Collaborator
University of Colorado, Denver	Colorado State University

Country where clinical trial is conducted

United States, 

References & Publications (3)

Caso G, McNurlan MA, Mileva I, Zemlyak A, Mynarcik DC, Gelato MC. Peripheral fat loss and decline in adipogenesis in older humans. Metabolism. 2013 Mar;62(3):337-40. doi: 10.1016/j.metabol.2012.08.007. Epub 2012 Sep 19. — View Citation

Kim JY, van de Wall E, Laplante M, Azzara A, Trujillo ME, Hofmann SM, Schraw T, Durand JL, Li H, Li G, Jelicks LA, Mehler MF, Hui DY, Deshaies Y, Shulman GI, Schwartz GJ, Scherer PE. Obesity-associated improvements in metabolic profile through expansion of adipose tissue. J Clin Invest. 2007 Sep;117(9):2621-37. — View Citation

Tchoukalova YD, Votruba SB, Tchkonia T, Giorgadze N, Kirkland JL, Jensen MD. Regional differences in cellular mechanisms of adipose tissue gain with overfeeding. Proc Natl Acad Sci U S A. 2010 Oct 19;107(42):18226-31. doi: 10.1073/pnas.1005259107. Epub 2010 Oct 4. — View Citation

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Change in adipose-derived stem cell (ASC) differentiation (Delta%oil red O+)	age-related difference in the ASC differentiation response to overfeeding	1 wk of overfeeding
Secondary	Change in ASC proliferation (Delta rate of cell growth)	age-related difference in the ASC proliferative response to overfeeding	1 wk of overfeeding
Secondary	Change in expression of C/EBPalpha, C/EBPbeta, PPARgamma2, SREBP1c, SREBf1a, and Wnt10b (fold-changes in mRNA expression)	age-related difference in the pro-adipogenic response to overfeeding	1 wk overfeeding
Secondary	Change in intramyocellular (IMCL) di-saturated diacylglycerol (DAG) localization (Delta %membrane:cytosol)	age-related difference in the IMCL DAG response to overfeeding	1 week overfeeding