Protein Kinase 2 (HIPK2) Polymorphisms on rs2058265, rs6464214, and rs7456421

Kidney Stone Clinical Trial

Official title:

Do Homeodomain Interacting Protein Kinase 2 (HIPK2) Polymorphisms rs2058265, rs6464214, and rs7456421 Associate With Nephrolithiasis in Turkish Population?

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT04804436
• Other study ID #: Geneticstone
• Status: Completed
• Phase: N/A
• Start date: August 1, 2018
• Est. completion date: October 6, 2019

Study information

• Verified date: March 2021
• Source: Saglik Bilimleri Universitesi
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

In the present study investigators aimed to investigate whether homeodomain interacting protein kinase 2 (HIPK2) polymorphism is associated with renal stone formation in Turkish population or not.

One hundred and twenty nine participants with calcium nephrolithiasis and 67 sex and age-matched healthy controls were enrolled in this study. For analysis of HIPK2 polymorphism, the real-time PCR amplification was performed in a final volume of 20μL reaction mixture, including 10 ng of genomic DNA, 5 µL of TaqMan® Universal PCR Master Mix, and 0.5 µL of 40X TaqMan® assay. The Rotor-Gene Q Series Software Version Q 2.3.1 (Rotor-Gene Q Series, Ziagen) was used for allelic discrimination. Chi square test was utilized to compare the differences of the genotype and allele frequencies between patients and controls.

Description:

Kidney stone incidence depends on geographical, climatic, ethnic, dietary and genetic factors. Thus the prevalence rates for urinary stones change from 1% to 20%. 1,2 Genetic polymorphism also causes nephrolithiasis. The most known polymorphic genes are the calcium-sensing receptor (CASR), vitamin D receptor (VDR), and matrix gla protein (MGP), plasminogen activator, urokinase (PLAU). 3,4 Furthermore, the concordance rate of the stone disease in monozygotic twins is substantially higher than in dizygotic ones (32.4% vs. 17.3%) demonstrating that genetic factors play a vital role in the formation of nephrolithiasis. 5 Homeodomain interacting protein kinase 2 (HIPK2) has been shown to be a new androgen receptor regulator. HIPK2 and androgen were demonstrated to mediate kidney tubular epithelial cell injury and apoptosis.

Informations on HIPK2 polymorphism about renal stone formation are newfound and inconclusive. Therefore, in this study, authors aimed to investigate whether HIPK2 polymorphism is associated with renal stone formation in Turkish population or not.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 196
• Est. completion date: October 6, 2019
• Est. primary completion date: October 6, 2019
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 38 Years to 68 Years

Eligibility

Inclusion Criteria:

Patients with nephrolithiasis

Exclusion Criteria:

• Patients had a history of chronic urinary tract infection

• renal failure

• gastrointestinal diseases

• increased levels of vitamin D

• sarcoidosis

• primary hyperoxaluria

• polycystic kidney disease, gout, renal tubular acidosis, primary and secondary hyperparathyroidism.

Related Conditions & MeSH terms

• Kidney Calculi
• Kidney Stone

Intervention

Genetic:
• Genetic analysis
he real-time PCR amplification was performed in a final volume of 20µL reaction mixture, including 10 ng of genomic DNA, 5 µL of TaqMan® Universal PCR Master Mix, and 0.5 µL of 40X TaqMan® assay. Thermal cycling conditions were as follows: initial denaturation at 94? for 3 min, 40 cycles of 94? for 15 s, and 60°C for 1 min. The Rotor-Gene Q Series Software Version Q 2.3.1 (Rotor-Gene Q Series, Ziagen) was used for allelic discrimination.

Locations

Country	Name	City	State
Turkey	Omer Gokhan Doluoglu	Ankara

Sponsors (1)

Lead Sponsor	Collaborator
Saglik Bilimleri Universitesi

Country where clinical trial is conducted

Turkey, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Single nucleotide polymorphism	Single nucleotide polymorphism incidence on rs2058265, rs6464214, and rs7456421	One year