Clinical Trial Details
— Status: Completed
Administrative data
NCT number |
NCT04804436 |
Other study ID # |
Geneticstone |
Secondary ID |
|
Status |
Completed |
Phase |
N/A
|
First received |
|
Last updated |
|
Start date |
August 1, 2018 |
Est. completion date |
October 6, 2019 |
Study information
Verified date |
March 2021 |
Source |
Saglik Bilimleri Universitesi |
Contact |
n/a |
Is FDA regulated |
No |
Health authority |
|
Study type |
Interventional
|
Clinical Trial Summary
In the present study investigators aimed to investigate whether homeodomain interacting
protein kinase 2 (HIPK2) polymorphism is associated with renal stone formation in Turkish
population or not.
One hundred and twenty nine participants with calcium nephrolithiasis and 67 sex and
age-matched healthy controls were enrolled in this study. For analysis of HIPK2 polymorphism,
the real-time PCR amplification was performed in a final volume of 20μL reaction mixture,
including 10 ng of genomic DNA, 5 µL of TaqMan® Universal PCR Master Mix, and 0.5 µL of 40X
TaqMan® assay. The Rotor-Gene Q Series Software Version Q 2.3.1 (Rotor-Gene Q Series, Ziagen)
was used for allelic discrimination. Chi square test was utilized to compare the differences
of the genotype and allele frequencies between patients and controls.
Description:
Kidney stone incidence depends on geographical, climatic, ethnic, dietary and genetic
factors. Thus the prevalence rates for urinary stones change from 1% to 20%. 1,2 Genetic
polymorphism also causes nephrolithiasis. The most known polymorphic genes are the
calcium-sensing receptor (CASR), vitamin D receptor (VDR), and matrix gla protein (MGP),
plasminogen activator, urokinase (PLAU). 3,4 Furthermore, the concordance rate of the stone
disease in monozygotic twins is substantially higher than in dizygotic ones (32.4% vs. 17.3%)
demonstrating that genetic factors play a vital role in the formation of nephrolithiasis. 5
Homeodomain interacting protein kinase 2 (HIPK2) has been shown to be a new androgen receptor
regulator. HIPK2 and androgen were demonstrated to mediate kidney tubular epithelial cell
injury and apoptosis.
Informations on HIPK2 polymorphism about renal stone formation are newfound and inconclusive.
Therefore, in this study, authors aimed to investigate whether HIPK2 polymorphism is
associated with renal stone formation in Turkish population or not.