Mitochondrial Function in Circulating Cells and Muscle Tissue

Healthy Clinical Trial

— MitoWomen  

Official title:

Mitochondrial Function in Circulating Cells and Muscle Tissue

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT02700698
• Other study ID #: 205139
• Status: Completed
• Start date: September 2016
• Est. completion date: May 1, 2018

Study information

• Verified date: October 2021
• Source: University of Arkansas
• Contact: n/a
• Is FDA regulated: No
• Study type: Observational

Clinical Trial Summary

The main goal of this project is to determine whether mitochondrial function in circulating cells is related to that measured in the muscle fibers of the same subjects.

Description:

Data from human and animal models strongly suggests that reduced tissue mitochondrial function can be an early marker for detection of dysfunctional metabolism. It is unclear if tissue mitochondrial dysfunction can be detected using less invasive methodology. In this project, the investigators plan to study mitochondrial bioenergetics in circulating cells and paired muscle biopsies in two metabolic disparate groups of women; healthy lean vs. overweight/obese with/without insulin resistant women of similar age. Women of similar age that are either healthy lean or overweight/obese with/without insulin resistance will be recruited to participate in one study that includes a blood draw and a muscle biopsy. Mitochondrial function in circulating blood cells and muscle tissue will be determined. The association between circulating blood cells and muscle tissue mitochondria respiration will be determined for the two groups combined, and differences between groups will also be investigated.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 35
• Est. completion date: May 1, 2018
• Est. primary completion date: April 26, 2018
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Female
• Age group: 25 Years to 35 Years

Eligibility

Inclusion Criteria:

• Women
• 25-35 years of age
• Either healthy lean (BMI<25) or insulin resistant and obese (BMI >30)

Exclusion Criteria:

• Medication that can affect study outcomes, as determined by the principal investigator, including anti-diabetic medication
• Chronic diseases, acute inflammation, chronic renal failure (glomerular filtration rate <45), or heart problems

Related Conditions & MeSH terms

• Healthy
• Insulin Resistance
• Insulin Sensitivity
• Obesity
• Women

Locations

Country	Name	City	State
United States	Arkansas Children's Nutrition Center	Little Rock	Arkansas
United States	UAMS	Little Rock	Arkansas

Sponsors (2)

Lead Sponsor	Collaborator
University of Arkansas	Arkansas Children's Hospital Research Institute

Country where clinical trial is conducted

United States, 

References & Publications (1)

Rose S, Carvalho E, Diaz EC, Cotter M, Bennuri SC, Azhar G, Frye RE, Adams SH, Børsheim E. A comparative study of mitochondrial respiration in circulating blood cells and skeletal muscle fibers in women. Am J Physiol Endocrinol Metab. 2019 Sep 1;317(3):E5 — View Citation

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Mitochondrial Function in Circulating Cells and Muscle Tissue in Young Women	Skeletal muscle (m.vastus lateralis) and peripheral blood mononuclear cell (PBMC) mitochondrial respiration were determined in response to substrate inhibitor coupling protocols using high resolution respirometry.; From this, mitochondrial respiration in leak and coupled states, and reserve respiratory capacity were determined in mitochondria from skeletal muscle and from PBMCs. It was determined whether mitochondrial function in circulating cells is related to that measured in the muscle tissue of the same subjects.	During the visit, approximately 0.5 days