Healthy Clinical Trial
Official title:
Study of Gene Expression in Fat and Muscle Tissue in Type 2 Diabetics and Healthy Controls During Experimental Endotoxemia
The aim of the present protocol is to study gene activity in fat and muscle tissue in type 2 diabetics and healthy volunteers after injection of E. coli endotoxin.We hereby hope to gain insight in some mechanisms behind the association between inflammation and insulin resistance.
Numerous studies have shown an association between low-grade inflammation and
insulin-resistance in type 2 diabetics. It is also well-known that septic patients often
develop insulin-resistance. The pathogenetic mechanisms behind the association between
inflammation and insulin-resistance is not fully understood.
In this study we create an experimental inflammation by giving E. coli endotoxin 0,3 ng/kg
iv to type 2 diabetics and healthy controls. Muscle biopsies are taken at 0, 2, 4 and 6
hours after injection, while fat biopsies are taken 0, 2, 4, 6 and 8 hours after endotoxin.
mRNA for adiponectin, leptin, PPAR-gamma, PGC-1, PAI-1 and cytokines in tissue is later
measured by RT-PCR.
Blood samples are drawn on an hourly basis until 8 hours after endotoxin injection for
common biochemical analyses including white blood cell count. Plasma is spared and later
analyzed for cytokines, PAI-1 and VCAM-1/ICAM-1.
;
Allocation: Non-Randomized, Endpoint Classification: Efficacy Study, Intervention Model: Single Group Assignment, Masking: Open Label, Primary Purpose: Prevention
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