Outcome
Type |
Measure |
Description |
Time frame |
Safety issue |
Primary |
Brachial artery flow-mediated dilation |
Percentage change in brachial artery diameter and return to baseline as assessed by ultrasound. |
Pre hot water immersion, then 1 hour, 3 hours, 24 hours and 48 hours post |
|
Secondary |
Brachial and carotid artery blood flow velocity |
Change from baseline in blood flow velocity of the brachial artery and right common carotid artery as assessed via ultrasound. cm/s is the unit. |
Pre hot water immersion, then 1 hour, 3 hours, 24 hours and 48 hours post |
|
Secondary |
Carotid vessel diameter |
Change in vessel diameter of the right common carotid artery and return to baseline as assessed via ultrasound. mm is the unit. |
Pre hot water immersion, then 1 hour, 3 hours, 24 hours and 48 hours post |
|
Secondary |
Carotid intima-media thickness |
Change in intima-media thickness of the right common carotid artery and return to baseline as assessed via ultrasound. mm is the unit. |
Pre hot water immersion, then 1 hour, 3 hours, 24 hours and 48 hours post |
|
Secondary |
Carotid compliance |
Change in compliance of the right common carotid artery and return to baseline as assessed via ultrasound. µm^2 per kilopascal is the unit. |
Pre hot water immersion, then 1 hour, 3 hours, 24 hours and 48 hours post |
|
Secondary |
Carotid distensibility |
Change in distensibility of the right common carotid artery and return to baseline as assessed via ultrasound. 10^-3/kPa is the unit. |
Pre hot water immersion, then 1 hour, 3 hours, 24 hours and 48 hours post |
|
Secondary |
Carotid stiffness |
Change in stiffness of the right common carotid artery and return to baseline as assessed via ultrasound. Meters per second is the unit. |
Pre hot water immersion, then 1 hour, 3 hours, 24 hours and 48 hours post |
|
Secondary |
Microvascular function |
Change in microvascular function and return to baseline as assessed via iontophoresis on the forearm with acetylcholine (Ach) and Insulin, Area under the curve is the unit. |
Pre hot water immersion, then 1 hour, 3 hours, 24 hours and 48 hours post |
|
Secondary |
Stroke volume |
Change in stroke volume and return to baseline measured noninvasively via thoracic impedance. mL/m^2 is the unit |
Pre hot water immersion, then 1 hour, 3 hours, 24 hours and 48 hours post |
|
Secondary |
Total peripheral resistance |
Change in total peripheral resistance and return to baseline measured noninvasively via thoracic impedance |
Pre hot water immersion, then 1 hour, 3 hours, 24 hours and 48 hours post |
|
Secondary |
Cardiac output |
Change in cardiac output and return to baseline measured noninvasively via thoracic impedance. L/min is the unit. |
Pre hot water immersion, then 1 hour, 3 hours, 24 hours and 48 hours post |
|
Secondary |
Intracellular HSP27 concentration |
Change in iHSP27 concentration and return to baseline. Measured via immunoblotting in peripheral blood mononuclear cells (PBMCs). |
Pre hot water immersion, then immediately post, 1 hour, 2 hours, 3 hours, 6 hours, 12 hours, 24 hours and 48 hours post |
|
Secondary |
Intracellular HSP60 concentration |
Change in iHSP60 concentration and return to baseline. Measured via immunoblotting in peripheral blood mononuclear cells (PBMCs). |
Pre hot water immersion, then immediately post, 1 hour, 2 hours, 3 hours, 6 hours, 12 hours, 24 hours and 48 hours post |
|
Secondary |
Intracellular HSP70 concentration |
Change in iHSP70 concentration and return to baseline. Measured via immunoblotting in peripheral blood mononuclear cells (PBMCs). |
Pre hot water immersion, then immediately post, 1 hour, 2 hours, 3 hours, 6 hours, 12 hours, 24 hours and 48 hours post |
|
Secondary |
Intracellular HSP90 concentration |
Change in iHSP90 concentration and return to baseline. Measured via immunoblotting in peripheral blood mononuclear cells (PBMCs). |
Pre hot water immersion, then immediately post, 1 hour, 2 hours, 3 hours, 6 hours, 12 hours, 24 hours and 48 hours post |
|
Secondary |
Plasma eHSP27 concentration |
Change in plasma eHSP27 concentration and return to baseline. Measured via ELISA. |
Pre hot water immersion, then immediately post, 1 hour, 2 hours, 3 hours, 6 hours, 12 hours, 24 hours and 48 hours post |
|
Secondary |
Plasma eHSP60 concentration |
Change in plasma eHSP60 concentration and return to baseline. Measured via ELISA. |
Pre hot water immersion, then immediately post, 1 hour, 2 hours, 3 hours, 6 hours, 12 hours, 24 hours and 48 hours post |
|
Secondary |
Plasma eHSP70 concentration |
Change in plasma eHSP70 concentration and return to baseline. Measured via ELISA. |
Pre hot water immersion, then immediately post, 1 hour, 2 hours, 3 hours, 6 hours, 12 hours, 24 hours and 48 hours post |
|
Secondary |
Plasma eHSP90 concentration |
Change in plasma eHSP90 concentration and return to baseline. Measured via ELISA. |
Pre hot water immersion, then immediately post, 1 hour, 2 hours, 3 hours, 6 hours, 12 hours, 24 hours and 48 hours post |
|
Secondary |
Reaction time |
Change in reaction time and return to baseline as assessed using ANAM: Automated Neurophysiological Assessment Metrics. |
Pre hot water immersion, then immediately post, 3 hours, 24 hours and 48 hours post |
|
Secondary |
Logical reasoning |
Change in logical reasoning and return to baseline as assessed using ANAM: Automated Neurophysiological Assessment Metrics. |
Pre hot water immersion, then immediately post, 3 hours, 24 hours and 48 hours post |
|
Secondary |
Memory |
Change in memory function and return to baseline as assessed using ANAM: Automated Neurophysiological Assessment Metrics. |
Pre hot water immersion, then immediately post, 3 hours, 24 hours and 48 hours post |
|
Secondary |
Cerebral oxygenation |
Change in cerebral oxygenation and return to baseline. Assessed using Near-infrared spectroscopy (NIRS) attached to the forehead |
Pre hot water immersion, then immediately post, 3 hours, 24 hours and 48 hours post |
|