The Effect of Exercise on Myokine Production in Aging Persons

Aging Clinical Trial

— EXI-AGE  

Official title:

Does an Acute Exercise Bout Increases Soluble and Muscle Myokine Concentration in Healthy, Older Persons

Clinical Trial Details — Status: Recruiting

Administrative data

• NCT number: NCT05571709
• Other study ID #: B1152022000006
• Status: Recruiting
• Phase: N/A
• Start date: September 15, 2022
• Est. completion date: December 31, 2023

Study information

• Verified date: March 2023
• Source: Hasselt University
• Contact: Bert Op'T Eijnde, Prof. dr.
• Phone: +3211292121
• Email: bert.opteijnde[@]uhasselt.be
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The incidence of age-related diseases, such as cancer and cardiovascular diseases, rapidly rises. These days, age-related diseases cause the majority of all healthcare expenses. During ageing senescent cells secrete a range of harmful inflammatory signals, which leads to chronic inflammation (inflamm-aging) and accelerates aging throughout the rest of the body. Interestingly, people who regularly exercise show less signs of inflamm-aging compared to people with a sedentary lifestyle. Molecules secreted by skeletal muscles, called myokines, may play an important role. However, the underlying molecular mechanisms remain unknown. This pilot study aims to investigate if an acute bout of resistance training is an appropriate tool to induce an increase in the release of muscle derived myokines in aged persons.

Recruitment information / eligibility

• Status: Recruiting
• Enrollment: 20
• Est. completion date: December 31, 2023
• Est. primary completion date: October 15, 2023
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 65 Years to 85 Years

Eligibility

Inclusion Criteria:

• Healthy persons

Exclusion Criteria:

• Smoking
• Infection < 2 weeks ago
• Received a vaccine < 2 weeks ago
• Allergy to Lidocain
• Immune deficiency
• Presence of a tumour or in treatment
• Usage of anti-coagulant medication
• Presence of following conditions:
• Haemophilia
• Auto-immune disease
• Cardiovascular disease
• Musculoskeletal diseases which refrains the patient from exercising

Related Conditions & MeSH terms

• Aging
• Exercise

Intervention

Other:
• Exercise training
Resistance training of the lower limbs

Behavioral:
• Questionnaire
Physical activity questionnaire

Procedure:
• Percutaneous muscle biopsy
Muscle biopsy of the vastus lateralis (m. quadriceps)
• Blood sample
Venous blood sample

Locations

Country	Name	City	State
Belgium	Hasselt University	Diepenbeek	Limburg

Sponsors (1)

Lead Sponsor	Collaborator
Hasselt University

Country where clinical trial is conducted

Belgium, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Cytokine concentration	Measurement of multiple cytokines in blood (for example: IL-6, IL-15, IL-4, irisin...) with ELISA's or multiplex assays	Day 2
Primary	Myokine production	Immunohistochemistry staining (fluorescent) of multiple myokines (for example: IL-6, IL-15, IL-4, irisin..) and aging markers (p16,p21...) on muscle tissue	Day 2
Primary	Immune parameters	Flow cytometry panels of immune parameters in muscle tissue	Day 2
Primary	Myokine protein levels	Western blotting of multiple myokines in muscle tissue	Day 2
Primary	Myokine gene expression	Quantitative PCR (polymerase chain reaction) of markers in muscle tissue	Day 2
Secondary	Blood pressure	The force of circulating blood on the walls of the arteries. Measured using an electronic sphygmomanometer	Day 1
Secondary	Resting Heart Rate	Measured using an electronic sphygmomanometer	Day 1
Secondary	International Physical Activity Questionnaire (Short form)	Measures the subjective level of activity of the last seven days. 7 questions regarding amount of time spent on certain activities.	Day 1