Eccentric Exercise and Oxidative Stress

Aging Clinical Trial

Official title:

The Effect of Eccentric Exercise on Oxidative Stress in the Elderly

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT01728675
• Other study ID #: EUC-017
• Secondary ID: EUC-019
• Status: Completed
• Phase: N/A
• First received: November 6, 2012
• Last updated: November 13, 2012
• Start date: May 2012
• Est. completion date: July 2012

Study information

• Verified date: November 2012
• Source: European University Cyprus
• Contact: n/a
• Is FDA regulated: No
• Health authority: Cyprus: Ministry of Health
• Study type: Interventional

Clinical Trial Summary

The purpose of the present investigation is to compare the responses and adaptations of young and elderly individuals to repeated eccentric exercise in regards to muscle function and redox homeostasis in a side-by-side comparison.

Description:

Despite the progress of analytic techniques and the refinement of study designs, striking disagreement exists among studies regarding the influence of exercise on muscle function and redox homeostasis in the elderly. A specific exercise protocol will be applied (the repeated eccentric model) to produce long-lasting and extensive changes in redox biomarkers and to examine more easily the potential effects of aging. Ten young men and ten elderly men will underwent an isokinetic eccentric exercise session, which will be repeated after three weeks. Muscle function indices and redox biomarkers will be assessed in urine, plasma or erythrocytes pre exercise, immediately post exercise, 2 and 4 days post exercise.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 20
• Est. completion date: July 2012
• Est. primary completion date: July 2012
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Male
• Age group: 18 Years to 70 Years

Eligibility

Inclusion Criteria:

• subject provides written informed consent

• physiological health profile

• stable at their anthropometric characteristics for at least the last year

Exclusion Criteria:

• muscle disease

• prior history of musculoskeletal injury to the lower limbs

• smokers

• consumed any nutritional supplement the last 3 months

• performed intense eccentric exercise the last 6 months

• non Caucasian

Study Design

Allocation: Non-Randomized, Intervention Model: Parallel Assignment, Masking: Open Label, Primary Purpose: Supportive Care

Related Conditions & MeSH terms

• Aging
• Muscle Damage
• Oxidative Stress

Intervention

Other:
• Exercise
Participants will perform two isokinetic eccentric exercise bouts

Locations

Country	Name	City	State
Cyprus	European University Cyprus	Nicosia

Sponsors (2)

Lead Sponsor	Collaborator
European University Cyprus	Aristotle University Of Thessaloniki

Country where clinical trial is conducted

Cyprus, 

References & Publications (5)

Nikolaidis MG, Jamurtas AZ. Blood as a reactive species generator and redox status regulator during exercise. Arch Biochem Biophys. 2009 Oct 15;490(2):77-84. doi: 10.1016/j.abb.2009.08.015. Epub 2009 Aug 25. Review. — View Citation

Nikolaidis MG, Kyparos A, Spanou C, Paschalis V, Theodorou AA, Vrabas IS. Redox biology of exercise: an integrative and comparative consideration of some overlooked issues. J Exp Biol. 2012 May 15;215(Pt 10):1615-25. doi: 10.1242/jeb.067470. Review. — View Citation

Nikolaidis MG, Kyparos A, Vrabas IS. F2-isoprostane formation, measurement and interpretation: the role of exercise. Prog Lipid Res. 2011 Jan;50(1):89-103. doi: 10.1016/j.plipres.2010.10.002. Epub 2010 Oct 15. Review. — View Citation

Panayiotou G, Paschalis V, Nikolaidis MG, Theodorou AA, Deli CK, Fotopoulou N, Fatouros IG, Koutedakis Y, Sampanis M, Jamurtas AZ. No adverse effects of statins on muscle function and health-related parameters in the elderly: an exercise study. Scand J Med Sci Sports. 2013 Oct;23(5):556-67. doi: 10.1111/j.1600-0838.2011.01437.x. Epub 2012 Jan 31. — View Citation

Theodorou AA, Nikolaidis MG, Paschalis V, Sakellariou GK, Fatouros IG, Koutedakis Y, Jamurtas AZ. Comparison between glucose-6-phosphate dehydrogenase-deficient and normal individuals after eccentric exercise. Med Sci Sports Exerc. 2010 Jun;42(6):1113-21. doi: 10.1249/MSS.0b013e3181c67ecd. — View Citation

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Muscle torque (torque)		Change in muscle torque from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise	No
Primary	Range of motion, ROM (degrees)		Change in ROM from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise	No
Primary	Delayed onset muscle soreness, DOMS (scale 1-10)		Change in DOMS from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise	No
Primary	Creatine kinase, CK (activity IU)		Change in CK from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise	No
Secondary	F2-isoprostanes (pg/mg creatinine)		Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise	No
Secondary	Protein carbonyls (nmol/mg protein)		Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise	No
Secondary	Glutathione, GSH (µmol/g Hb)		Change in GSH from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise	No
Secondary	Catalase (U/g Hb)		Change in catalase from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise	No
Secondary	Superoxide dismutase, SOD (U/g Hb)		Change in SOD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise	No
Secondary	Glutathione peroxidase, GPx (U/g Hb)		Change in GPx from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise	No
Secondary	Glucose-6-phosphate dehydrogenase, G6PD (U/g Hb)		Change in G6PD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise	No
Secondary	Uric acid (mM)		Change in uric acid from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise	No
Secondary	Albumin (µM)		Change in albumin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise	No
Secondary	Bilirubin (µM)		Change in bilirubin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise	No