Clinical Trial Details
— Status: Completed
Administrative data
NCT number |
NCT04141618 |
Other study ID # |
BR-100-006 |
Secondary ID |
|
Status |
Completed |
Phase |
|
First received |
|
Last updated |
|
Start date |
August 2011 |
Est. completion date |
July 31, 2015 |
Study information
Verified date |
October 2019 |
Source |
National Cheng-Kung University Hospital |
Contact |
n/a |
Is FDA regulated |
No |
Health authority |
|
Study type |
Observational
|
Clinical Trial Summary
Development of mole was not associated with segregation of mutated NLRP7 allele in the
haploid oocyte. We hypothesize NLRP7 is a maternal factor involved in regulating early embryo
development or embryo-uterine interaction. In the proposed study, we seek to identify novel
genetic variants and mutations of NLRP7 in women who experienced RM/HM. Genetic association
study and haplotype analysis are performed to test assocation between NLRP7 gene and female
reproductive performance. Immunohistochemical staining, RT-PCR, and Western blot analysis are
used to investigate expression pattern of NLRP7 in endometrium and placenta. Two approaches
are used to characterize functional significance of genetic variants/mutations. The first
approach will be based on mutagenesis and the second approach will be based on induced
pluripotent stem cells (iPSCs). Results obtained from the proposed study will provide novel
insight into mechanism of embryo development and implantation.
Description:
Recurrent miscarriage (RM), defined as at least two consecutive fetal death or spontaneous
abortions before the 20th week gestational age, occurred in about 1% to 5% couples. The
causes of RM include uterine factors, endocrine factors, thromobophilic factors, immunologic
factors and genetic factors. The hydatidiform mole (HM) can be divided into two separate
syndromes: complete mole (CHM) and partial mole (PHM). In the West, CHMs occur in
approximately 1 in every 1500 pregnancies. The incidence is higher in Latin America,
Southeast Asia and the Middle East. The cause of RM and HM are not known for the vast
majority of cases. The NLRP (Nucleotide-binding oligomerization domain, Leucine rich Repeat
and Pyrin domain containing) family, also referred to as NALP family, is well known for its
roles in apoptosis and inflammation. Expression studies showed that twelve of the fourteen
members of NLRP family express differentially in human oocytes and preimplantation embryonic
cells, especially NLRP7, indicating important role of NLRP family in female reproduction.
Since 2006, mutations of NLRP7 have been found in women with repeated occurrence of molar
pregnancy, repeated stillbirth or early spontaneous abortion. In women who experienced RM/HM,
we also found some novel mutations/genetic variants of NLRP7. Taken together, these finding
suggest RM and HM may share the same genetic etiology in some cases. In addition, NLRP7 is a
strong candidate gene for RM/HM. A recent report showed chaotic cleavage abnormalities of
embryos in patients with NLRP7 variants.