Clinical Trial Details
— Status: Not yet recruiting
Administrative data
| NCT number |
NCT05658445 |
| Other study ID # |
As |
| Secondary ID |
|
| Status |
Not yet recruiting |
| Phase |
|
| First received |
|
| Last updated |
|
| Start date |
January 1, 2023 |
| Est. completion date |
March 1, 2023 |
Study information
| Verified date |
December 2022 |
| Source |
Assiut University |
| Contact |
n/a |
| Is FDA regulated |
No |
| Health authority |
|
| Study type |
Observational [Patient Registry]
|
Clinical Trial Summary
The definition of recurrent spontaneous abortion (RSA) has changed over the years, and most
societies now advocate defining RSA as two or three consecutive or discontinuous miscarriages
with the same sexual partner before 24 weeks gestation
In recent years, the incidence of this disease has been on the rise, occurring in about 1%-
5% of pregnancy in women at childbearing age, and the success rate of second pregnancy in RSA
females has been significantly reduced The etiology of RSA is extremely complex, including
anatomical factors, genetic factors, endocrine factors, infectious and immune factors, and
pre-thrombosis etiology.
However, the cause of the disease is unclear in half of patients and known as unexplained
recurrent spontaneous abortion (URSA)
Description:
- MicroRNA (miRNA) is a kind of non-coding single-stranded RNA molecule encoded by
endogenous genes with a length of about 22 nucleotides . MiRNA negatively regulates gene
expression mainly by binding to the 3 'untranslated region (3' UTR) of target mRNA to
degrade the target mRNA or inhibit its translation . In recent years, there have been
numerous reports about miRNA's involvement in the pathogenesis of RSA, and many of them
have focused on how miRNA regulates trophoblast function.
- Additionally, microRNA (miR)-410-5p has been discovered to serve as oncogenes and tumor
suppressors altering cell functions in numerous ways including proliferation, apoptosis,
biochemistry metabolism, and inflammatory responses in different human malignancies,
such as liver cancer, pancreatic cancer, colon cancer, and non-small cell lung cancer.
Recent studies showed that miR-410-5p may be involved in the non-cancerous disorder
processes such as RSA . Overexpression of miR-410-5p in trophoblast cells inhibited the
polarization of M2 macrophages, while knockdown of miR-410-5p was beneficial to
recruitment of trophoblast cell and promoted the polarization of M2 macrophages.
Furthermore, MicroRNA (miR)-410-5p was discovered to bind with 3'-UTR of ITGA6 .
- The adhesion molecule integrin alpha-6 (ITGA6, CD49f), a member of the integrin family,
is overexpressed in many cancers and enhances cell movement and signal output .
- Down-regulation of ITGA6 changed the biological function of trophoblast cells, inhibited
cell proliferation, invasion and migration, and induced apoptosis. ITGA6 may affect the
biological functions of trophoblast cells by regulating PI3K/AKT and MAPK signaling
pathways. MAPK signaling promote apoptosis through inducing releasing of cytochrome c,
which in turn induces further caspase activation (caspase-9 and the effector caspases-3,
-6, and -7) .
- Baculoviral IAP Repeat Containing 7(BIRC7) encodes the protein Livin which is a member
of the inhibitor of apoptosis protein (IAP) family. Livin consists of a single
baculoviral IAP repeat domain (BIR) and a RING domian at the C-terminus. The protein
inhibits apoptosis by inhibiting proteolytic activation of capsases.
- Currently, the interaction of BIRC7 and NK cells in endometrium/decidual remains
unknown. BIRC7 in decidua cell may possess the ability to prevent NK cells from killing
embryonic and extraembryonic cells, decreased level of BIRC7 and increased number of NK
cells in the RM group attribute to the occurrence of miscarriage
