Uveitis Sarcoid Clinical Trial
Official title:
Observational Study: Definition of Immunologic Markers for the Differential Diagnosis Between Uveitis From Supposed Tuberculous Etiology and Sarcoidosis Uveitis
Today there are no tests that allow to make a precise differential diagnosis between uveitis from presumed tuberculous origin and uveitis by sarcoidosis. Therefore, with this study, investigators aim to identify, in the aqueous humor and in the blood of participants (patients that suffering from one of these two forms of uveitis) the presence of immunologic markers that distinguish between uveitis of tuberculous etiology and uveitis by sarcoidosis.
Both tuberculosis and sarcoidosis are chronic, multi-systemic and granulomatous pathologies
that have very similar pulmonary and extra-pulmonary manifestations, and even in the case of
ocular involvement it has been shown that many features of intraocular TB can also be found
in participants with ocular sarcoidosis.
In this monocentric observational study investigators are asking to the participants with
granulomatous uveitis, in which a tuberculosis or sarcoidosis origin is suspected and who
will have undergo paracentesis of the anterior chamber, to grant part of the aqueous humor
and a blood sample for this study.
The collected samples will be analyzed as follow:
1. Determination of the concentration of various cytokines, chemokines and growth factors
in aqueous humor and in the plasma.
2. Analysis of the mononuclear cells ( T helper lymphocytes CD3+ (CD3 is the acronym of
cluster of differentiation 3: a glycoprotein found on the surface of immune cells) and
CD4+ ((CD4 is the acronym of cluster of differentiation 4), T cytotoxic lymphocytes CD3+
CD8+ (CD8 is the acronym of cluster of differentiation 8) , B lymphocytes CD19+ (CD19 is
the acronym of cluster of differentiation 19), Natural killer lymphocytes CD56+ (CD56 is
the acronym of cluster of differentiation 56) and CD3- and monocytes CD14+ (CD14 is the
acronym of cluster of differentiation 14) present in the aqueous humor by
cytofluorimetry.
3. Evaluation of the presence of anti-human HSP70 antibodies in plasma samples by Western
blot immunoprecipitation assays.
4. Whenever possible (adequate number of cells), in vitro stimulation of mononuclear cells
in aqueous humor with epitopes of tuberculosis mycobacterium antigens and the analysis
of cytokines production in the supernatants.
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