Bacteria Elimination in Retreatment Cases Using Different Disinfection Techniques

Root Canal Infection Clinical Trial

Official title:

Enterococcus Faecalis Elimination in Retreatment Cases Using Passive Ultrasonic Irrigation (PUI), Manual Dynamic Activation (MDA) and Photodynamic Therapy (PDT): a Randomized Clinical Trial

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT03750162
• Other study ID #: 2016-27194235-02
• Status: Completed
• Phase: N/A
• Start date: February 1, 2017
• Est. completion date: September 5, 2017

Study information

• Verified date: November 2018
• Source: Bulent Ecevit University
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

Nonsurgical root canal retreatment may become essential when the initial endodontic treatment fails because of the persistent intracanal or extracanal infections.It has been stated that the one of the main cause of posttreatment disease is the persistent or reintroduced microorganism such as enterococcus faecalis. Supplementary disinfection techniques such as irrigation agitation methods can help elimination of bacteria from root canal systems.

The aim of this in vivo study was to compare the effect of passive ultrasonic irrigation, manual dynamic activation and photodynamic therapy in reducing bacterial load in endodontic retreatment cases with periradicular lesion by using polymerase chain reaction (PCR)in a single visit root canal treatment.

Description:

Thirty-six teeth with a single root and a single canal with posttreatment apical periodontitis which needed retreatment were selected for this study. Teeth were randomly divided into 3 groups according to the supplementary disinfection techniques used: Passive ultrasonic irrigation, Manual dynamic activation and Photodynamic therapy. Microbiological samples were taken before any intervention, after conventional chemo-mechanical preparation, and after disinfection techniques with sterile paper points and held in thioglycollate broth, and then were transferred to the microbiological laboratory. The amounts of e.faecalis in these samples was measured by using PCR.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 36
• Est. completion date: September 5, 2017
• Est. primary completion date: June 15, 2017
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 14 Years to 65 Years

Eligibility

Inclusion Criteria:

Patients with no clinical symptoms Endodontically treated teeth Single rooted teeth

Exclusion Criteria:

Teeth with fractured instrument, resorption, perforation, ledge, over fillings Periodontal pockets deeper than 4 mm

Related Conditions & MeSH terms

• Communicable Diseases
• Infection
• Periapical; Infection
• Root Canal Infection

Intervention

Device:
• VDW Ultra
supplementary disinfection technique
• AMD picasso
supplementary disinfection technique
• ProtaperNext X3 gutta-percha
supplementary disinfection technique

Locations

Country	Name	City	State
Turkey	Bulent Ecevit University Faculty of Dentistry Department of Endodontics	Zonguldak

Sponsors (1)

Lead Sponsor	Collaborator
Bulent Ecevit University

Country where clinical trial is conducted

Turkey, 

References & Publications (2)

Asnaashari M, Godiny M, Azari-Marhabi S, Tabatabaei FS, Barati M. Comparison of the Antibacterial Effect of 810 nm Diode Laser and Photodynamic Therapy in Reducing the Microbial Flora of Root Canal in Endodontic Retreatment in Patients With Periradicular Lesions. J Lasers Med Sci. 2016 Spring;7(2):99-104. doi: 10.15171/jlms.2016.17. Epub 2016 Mar 27. — View Citation

Rodrigues RC, Antunes HS, Neves MA, Siqueira JF Jr, Rôças IN. Infection Control in Retreatment Cases: In Vivo Antibacterial Effects of 2 Instrumentation Systems. J Endod. 2015 Oct;41(10):1600-5. doi: 10.1016/j.joen.2015.06.005. Epub 2015 Jul 30. — View Citation

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Bacteria change	Bacteria samples were taken at 3 step; before any intervention, after chemomechanical preparation, and after applying supplementary disinfection techniques	1 year