Outcome
Type |
Measure |
Description |
Time frame |
Safety issue |
Other |
Difference in CRF concentrations between the two exposures |
Difference in the serum concentrations of corticotrophin releasing factor between ozone exposure and filtered air exposure |
2 hours after the completion of exposure. |
|
Other |
Difference in ACTH concentrations between the two exposures |
Difference in the serum concentrations of adrenocorticotropic hormone between ozone exposure and filtered air exposure |
2 hours after the completion of exposure. |
|
Other |
Difference in cortisol concentrations between the two exposures |
Difference in the serum concentrations of cortisol between ozone exposure and filtered air exposure |
2 hours after the completion of exposure |
|
Other |
Difference in adrenaline concentrations between the two exposures |
Difference in the serum concentrations of adrenaline between ozone exposure and filtered air exposure |
2 hours after the completion of exposure. |
|
Other |
Difference in noradrenaline concentrations between the two exposures |
Difference in the serum concentrations of noradrenaline between ozone exposure and filtered air exposure |
2 hours after the completion of exposure. |
|
Other |
Change in the amount of skin moisture |
Change in the amount of skin moisture, which was measure by a moisture meter. The number on the meter indicates how hydrated (or dehydrated) your skin is. |
Immediately before exposure, 0.5 hours after the completion of exposure, and next morning. |
|
Other |
Change in L-values |
Change in skin color indicated by L-values, which was measured by a skin colorimeter. |
Immediately before exposure, 0.5 hours after the completion of exposure, and next morning. |
|
Other |
Change in a-values |
Change in skin color indicated by a-values, which was measured by a skin colorimeter. |
Immediately before exposure, 0.5 hours after the completion of exposure, and next morning. |
|
Other |
Change in b-values |
Change in skin color indicated by b-values, which was measured by a skin colorimeter. |
Immediately before exposure, 0.5 hours after the completion of exposure, and next morning. |
|
Other |
Difference in alpha diversity of microbiota between the two exposures |
Difference in alpha diversity of microbiota in nasal secretions between ozone exposure and filtered air exposure |
Immediately after the completion of exposure |
|
Other |
Change in CC16 concentrations |
Change in the serum concentrations of club cell protein |
Immediately before exposure, 2 hours after the completion of exposure, and next morning |
|
Other |
Differences in metabolite levels detected in serum metabolomics between the two exposures |
Mass spectrometry-based serum metabolomics is non-targeted.The study is to find the differential metabolites in serum between ozone exposure and filtered air exposure. |
2 hours after the completion of exposure |
|
Other |
Differences in metabolite levels detected in airway metabolomics between the two exposures |
Mass spectrometry-based metabolomics in exhaled breath condensate is non-targeted.The study is to find the differential metabolites in airway between ozone exposure and filtered air exposure. |
2 hours after the completion of exposure |
|
Other |
Differences in metabolite levels detected in urine metabolomics between the two exposures |
Mass spectrometry-based urine metabolomics is non-targeted.The study is to find the differential metabolites in urine between ozone exposure and filtered air exposure. |
Immediately after the completion of exposure |
|
Other |
Differences in RNA expression levels detected in transcriptomics between the two exposures |
Illumina-based transcriptomics is non-targeted. The study is to find the differentially expressed RNA between ozone exposure and filtered air exposure. |
2 hours after the completion of exposure |
|
Other |
Differences in protein levels detected in serum proteomics between the two exposures |
Mass spectrometry-based proteomics in serum is non-targeted. The study is to find the differentially expressed proteins in serum between ozone exposure and filtered air exposure. |
2 hours after the completion of exposure |
|
Primary |
Changes of FEV1 |
Changes of forced expiratory volume in 1 second |
FEV1 will be examined immediately prior to exposure, immediately after exposure, 2 hours after exposure and again the next morning |
|
Primary |
Changes of FVC |
Changes of forced vital capacity |
FVC will be examined immediately prior to exposure, immediately after exposure, 2 hours after exposure and again the next morning |
|
Secondary |
HRV |
Heart rate variability |
Holter monitoring will be performed continuously for 24 hours. |
|