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Single-Cell Transcriptomics of the Peritoneal Microenvironment of Colorectal PC — SingleCell  

Peritoneal Metastases Clinical Trial

Official title:
The Role of the Peritoneal Microenvironment in the Pathogenesis of Colorectal Peritoneal Carcinomatosis: Single-cell Transcriptomics

Clinical Trial Summary

Single cell transcriptomics of CRC PC samples

Clinical Trial Description

Research methodology Before inclusion of patients, patients will be screened for the presence of hepatitis B, hepatitis C and HIV to ensure researcher safety. During cytoreductive surgery, a total of five samples of tumoral tissue will be acquired. Three of those samples will be utilized for ScRNASeq analysis, and two samples of a macrometastasis will be snap frozen and cryopreserved. Single-cell RNA-Sequencing analysis We will comprehensively catalog the stromal cell types in the microenvironment of colorectal cancer peritoneal metastases utilizing single-cell RNA sequencing (scRNAseq) on three samples (one of which should preferably originate from the omentum majus, to encompass milky spots) acquired from surgical waste material of patients undergoing cytoreductive surgery. After sample prelevation, samples will be transported as soon as possible while cooled and submerged in 10% PBS to the VIB facilities, where single cell suspensions will be made according to standard protocols. After cell selection by FACS (CD45+ and live/dead stain, to ensure a 50/50 mix of immune and stromal cells) and library prep, ScRNASeq will be performed. Single cell sequencing analysis will allow us to identify cell clusters that by usage of marker genes will be assigned to different known cell lineages. After annotation of data, a "landscape" of the composition of the tumor stroma will be acquired. ;

Study Design

Related Conditions & MeSH terms

Clinical Trial Details
NCT number NCT05676372
Study type Observational
Source University Hospital, Ghent
Contact Wim P Ceelen, MD, PhD
Phone 093326251
Email wim.ceelen@ugent.be
Status Recruiting
Phase
Start date June 16, 2020
Completion date December 31, 2025
View Details
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