Cell Block Immunohistochemistry in Effusion Cytology

Status Recruiting

Clinical Trial Summary

Cytology is usually the first step in investigating serous effusions, to either detect or exclude an underlying malignancy. This study will try to answer the need for improved diagnostic yield of cytologic examination by cell block technique and immunohistochemical testing of three markers which are EZH2, Claudin-4 and MOC-31.

Clinical Trial Description

This is a prospective study that will be conducted at the pathology lab of Assiut University Hospital. The study will include effusion specimens (pleural, peritoneal & pericardial) and the aspirated fluid will be examined for physical characters e.g. color and appearance.

The sample will be divided into 2 equal parts: one for conventional smear preparation, and the other for cell block. A minimum of 2 thin smears will be prepared and stained with Papanicolou stain.

Cell block sections of 4-6 μ thickness will be prepared and stained with the hematoxylin and eosin stain (H&E).

For immunohistochemistry, cell blocks will be used. Expression of claudin 4, EZH2, and MOC-31 will be evaluated in benign and malignant cells by using immunoperoxidase technique. ;

Study Design

Related Conditions & MeSH terms

Clinical Trial Details

NCT number	NCT04279327
Study type	Observational
Source	Assiut University
Contact	Aliaa EM Mahmoud, MSc
Phone	+201007508736
Email	aliaa.elhosainy88@yahoo.com
Status	Recruiting
Phase
Start date	April 1, 2020
Completion date	January 2022

View Details

See also
Status	Clinical Trial	Phase
Recruiting	`NCT02891642` - Liquid Biopsy With Immunomagnetic Beads Capture Technique for Malignant Cell Detection in Body Fluid
Recruiting	`NCT00239096` - Prevention of Decompensation in Liver Cirrhosis	Phase 4
Active, not recruiting	`NCT03973866` - Alfapump® System in the Treatment of Refractory or Recurrent Ascites (POSEIDON Study)	N/A
Terminated	`NCT01455246` - Daptomycin + Meropenem Versus Ceftazidime in the Treatment of Nosocomial Spontaneous Bacterial Peritonitis	Phase 2/Phase 3
Completed	`NCT01349348` - Phase III Study of Tolvaptan Tablet to Treat Cirrhosis Ascites	Phase 3
Completed	`NCT01578226` - Procalcitonin in Cirrhotic Patients at High Risk for Sepsis	N/A
Terminated	`NCT00548366` - Sodium Restriction in the Management of Cirrhotic Ascites	Phase 4
Recruiting	`NCT05025878` - 13C-Glucose Tracing of Tumour and T Cells in the Ascites of Ovarian Cancer Patients.
Completed	`NCT03327688` - Point-of-care Ultrasound in Finland	N/A
Not yet recruiting	`NCT04550091` - Role Of Multi-detector Computed Tomography In Differentiation Between Different Types Of Ascites
Completed	`NCT01769040` - Intestinal Decontamination With Rifaximin. The Inflammatory and Circulatory State in Patients With Cirrhosis	Phase 4
Not yet recruiting	`NCT01716611` - Tolvaptan for Hyponatremia in Cirrhotic Patients With Ascites	Phase 4
Recruiting	`NCT05700708` - Point-of-Care Echocardiography to Assess Impact of Dynamic Cardiac Function, Renal and Cardiac Biomarkers in Cirrhosis With Refractory Ascites
Recruiting	`NCT05511766` - Allopurinol Versus Atorvastatin to Prevent Complications of Liver Cirrhosis	Phase 2/Phase 3
Completed	`NCT05013502` - Empagliflozin in Diuretic Refractory Ascites	Phase 1
Not yet recruiting	`NCT06436807` - PMCF Study of the CE-marked Drainova® ArgentiC Catheter
Completed	`NCT03263598` - Validation of Diagnostic Usefulness of the Random Urine Na/K Ratio for Replacement of 24hr Urine Na Excretion in Cirrhotic Patients With Ascites
Completed	`NCT00907673` - The Automated Fluid Shunt (AFS)in Chronic Congestive Heart Failure	N/A
Suspended	`NCT00511394` - Acute Hemodynamics of Albumin Versus Normal Saline in Cirrhosis	N/A
Terminated	`NCT00796861` - Trial of Sunitinib for Refractory Malignant Ascites	Phase 2