| Eligibility |
Inclusion Criteria:
1. Subjects must have the ability to understand and the willingness to sign a written
informed consent document
2. Stated willingness to comply with all study procedures and availability for the
duration of the study
3. Male or female, between the age of 1 and 65 years
4. In good general health as evidenced by medical history and diagnosed relapsed or
refractory CD19+ B cell malignancies including acute lymphocytic leukemia (ALL),
non-Hodgkin's lymphoma containing Diffuse large B-cell lymphoma (DLBCL), primary
mediastinal large B-cell lymphoma (PMBCL), mantle cell lymphoma (MCL), follicular
lymphoma (FL), or small lymphocytic lymphoma (SLL), B-cell prolymphocytic
leukemia(BPLL), Lymphoplasmacytic Lymphoma, Marginal Zone Lymphoma (MZL).
5. Definition of relapse or refractory as below:
- Ineligible hematopoietic transplantation
- Relapse after transplantation
- Subject with ALL was evaluated by minimal residual disease > 0.1% after treatment
with at least two lines of therapy. Subjects with Philadelphia Chromosome
positive acute lymphoblastic leukemia (Ph+ALL) treated by at least two lines of
therapy, including tyrosine kinase inhibitors (TKIs) are eligible.
- Subject with lymphoma treated regimens containing both anti-CD20 antibody and
anthracycline-containing chemotherapy regiment. Subjects with transformed
follicular lymphoma must have received prior chemotherapy for follicular lymphoma
and subsequently have the chemo refractory disease after transformation to
diffuse large B-cell lymphoma
6. The patient's disease must be CD19 positive, either by immunohistochemistry or flow
cytometry analysis on the last biopsy available.
7. Performance status: Adult Subjects: ECOG =2; Subjects > 10 years of age: Karnofsky=
50%; Subjects = 10 years of age: Lansky scale = 50% (See Appendix 1)
8. The following laboratory values :
- Total bilirubin = 2x upper limit of normal
- AST (SGOT) = 5x upper limit of normal
- ALT (SGPT) = 5x upper limit of normal
- Serum Creatinine = 2.0 mg/dl
- Subjects must have the following hematologic function parameters (supportive care
is allowed per institutional standards, i.e. filgrastim, transfusion):
i.Absolute lymphocyte count>0.3× 109cells/L ii.Platelets>50 × 109 cells/L
9. Prior therapy wash-out: At least 2 weeks or 5 half-lives, whichever is longer, must
have elapsed since any prior systemic therapy including cytotoxic chemotherapy,
immunomodulatory, immunosuppressive, antiproliferative drugs, antibody, immune
checkpoint therapy, pegylatedasparaginase, pegfilgrastimat the time the subject is
planned for leukapheresis. Filgrastim, intrathecal methotrexate, systemic steroid, and
donor lymphocyte infusion must be stopped 5 days, 7 days, 72 hours, and 4 weeks before
leukapheresis, respectively.
10. Ability to take oral medication and be willing to adhere to the intervention protocols
and medication regimens
11. For females of reproductive potential: use of highly effective contraception for at
least 1 month prior to enrollment and agreement to use such a method during the
follow-up period of the protocol
12. For males of reproductive potential: use of condoms or other methods to ensure
effective contraception with partner
Exclusion Criteria:
1. Current use of systemic steroids or chronic use of immunosuppressant medications.
Recent or current use of inhaled steroids is not exclusionary.
2. Pregnancy or lactation
3. Known allergic reactions to components used in the intervention protocols or
medication regimens in this study
4. Active Febrile illness
5. Treatment with another investigational drug or other intervention within 30 days or 5
half-lives.
6. Autologous transplant within 6weeks of planned CAR-T cell infusion.
7. Patients who are able to obtain market approved CD19 CAR T-cell therapies.
8. Active central nervous system or meningeal involvement by tumor. Subjects with
untreated brain metastases/CNS disease will be excluded from this clinical trial
because of their poor prognosis and because they often develop progressive neurologic
dysfunction that would confound the evaluation of neurologic and other adverse events.
Patients with a history of CNS or meningeal involvement must be in a documented
remission by CSF evaluation and contrast-enhanced MRI imaging for at least 90 days
prior to Enrollment.
9. History of active malignancy other than non-melanoma skin cancer, carcinoma in situ
(e.g. cervix, bladder, breast).
10. Active HIV infection.
11. Subjects with uncontrolled intercurrent illness including, but not limited to ongoing
or active infection, symptomatic congestive heart failure, unstable angina pectoris,
cardiac arrhythmia, pulmonary abnormalities or psychiatric illness/social situations
that would limit compliance with study requirements.
12. Pregnant or breastfeeding women are excluded from this study because CAR-T cell
therapy may be associated with the potential for teratogenic or abortifacient effects.
Women of child-bearing potential must have a negative serum pregnancy test. Because
there is an unknown, but potential risk for adverse events in nursing infants
secondary to treatment of the mother with CAR-T cells, breastfeeding should be
discontinued. These potential risks may also apply to other agents used in this study.
Subjects of child-bearing or child-fathering potential must be willing to practice
birth control from the time of enrollment on this study to the follow-up period of the
protocol.
13. Evidence of myelodysplasia or cytogenetic abnormality indicative of myelodysplasia on
any bone marrow biopsy prior to initiation of therapy
14. Serologic status reflecting active hepatitis B or C infection. Patients that are
positive for hepatitis B core antibody, hepatitis B surface antigen (HBsAg), or
hepatitis C antibody must have a negative viral load prior to enrollment. (viral load
positive patients will be excluded.)
15. Severe concomitant disease or organic dysfunction that is expected to reduce life
expectancy less than 3 months.
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