Taurine Supplementation and Exercise on Irisin Levels in Obesity

Obesity Clinical Trial

Official title:

Effects of Taurine Supplementation Associated With High Intensity Physical Training on Irisin Levels, Energy Expenditure and Body Composition of Obese Women

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT04646512
• Other study ID #: 51921115500005426
• Status: Completed
• Phase: N/A
• Start date: February 1, 2016
• Est. completion date: August 1, 2016

Study information

• Verified date: November 2020
• Source: University of Sao Paulo
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

Irisin is a myocin secreted by skeletal muscle in stimulus to physical exercise and has been described as a possible therapeutic tool in the fight against obesity by triggering a cascade of signaling that triggers the expression of genes responsible for the increase in energy expenditure and browning of adipose tissue white cells by activating thermogenesis promoter mitochondrial proteins. Although the science is engaged in the search for evidences about the actions of the irisin, the physical exercise involved and the improvement in the levels of obesity, many mechanisms are still unknown. Since taurine by means of irisin-like pathways also increases energy expenditure, it is believed that taurine supplementation associated with high intensity aerobic physical exercise can optimize the effects of irisin, increasing energy expenditure and improving body composition in obesity.

Description:

Introduction: Irisin is a myocin secreted by skeletal muscle when stimulated by physical exercise that promotes increased thermogenesis (browning effect). Similarly, taurine is also associated with modulations of energy metabolism with promising actions in the control of metabolic disorders. Thus, it is believed that chronic taurine supplementation associated with physical training can optimize irisin concentrations after exercise. Objective: Evaluate the effects of taurine supplementation associated with interval aerobic training on plasma irisin concentrations, energy expenditure, body composition and physical fitness in obese women. Methods: Twenty-two women aged 25 to 45 years, sedentary, with grade I obesity (BMI ≥30-35 kg / m²) and without comorbidities will be recruited for the study. The subjects will be submited to a high intensity interval aerobic training for 8 weeks, being 3 times by week, for 50 minutes, associated to the supplementation with 3g of taurine or placebo. Methods: Assessment of dietary intake, Resting Energy Expenditure (GER) by indirect calorimetry, body composition by deuterium oxide, anthropometric measurements, HPLC plasma analysis of taurine, multiplex irisin and physical fitness variables will be performed pre and post intervention. The results will be expressed as mean and standard deviation and the ANOVA test two way repeated measures mixed model will be applied, with post hoc Sidak, to verify differences and statistical interactions (p <0.05).

Recruitment information / eligibility

• Status: Completed
• Enrollment: 31
• Est. completion date: August 1, 2016
• Est. primary completion date: May 1, 2016
• Accepts healthy volunteers: No
• Gender: Female
• Age group: 25 Years to 45 Years

Eligibility

Inclusion Criteria:

• This study included women with obesity grade I (BMI =30 - =35Kg/m²), sedentary, with no associated comorbidity, convenience sample.

Exclusion Criteria:

• Women classified as overweight, obesity grade II and grade III (morbid), who present comorbidities associated with obesity (pre-diabetes, diabetes, hypertension, dyslipidemia, cardiovascular diseases and diseases of bone metabolism); smokers; who use hormones, sports supplements and / or weight loss medications. Perform some type of nutritional monitoring followed by diet or weight loss guidelines and that have medical impediment to the practice of physical exercise.

Related Conditions & MeSH terms

• Obesity

Intervention

Dietary Supplement:
• Taurine group and exercise and placebo group and exercise
Both groups supplemented with taurine and / or placebo will perform physical training.

Other:
• Physical training with Deep Water Running
There will be a physical training for eight weeks.

Locations

Country	Name	City	State
Brazil	Gabriela Batitucci	Ribeirão Preto	SP

Sponsors (1)

Lead Sponsor	Collaborator
University of Sao Paulo

Country where clinical trial is conducted

Brazil, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Changes body weight	The body weight was measured by digital balance before and after the intervention	Two times: (1) First day and (2) 10 weeks after assessments, adaptation and intervention
Primary	Changes body composition	The body composition was evaluated through deuterium oxide	Two times: (1) First day and (2) 10 weeks after assessments, adaptation and intervention
Primary	Changes indirect calorimetry	With a gas analyzer (indirect calorimeter), we evaluated the metabolic rate and rest (REE) and oxidation of substrates (lipids and carbohydrates)	Two times: (1) First day and (2) 10 weeks after assessments, adaptation and intervention
Primary	Changes in total cholesterol and lipid fractions	Collected in EDTA tubes, were centrifuged.	Two times: (1) First day and (2) 10 weeks after assessments, adaptation and intervention
Primary	Changes in plasma taurine concentration	Collected in EDTA tubes, were centrifuged and the plasma taurine concentration was evaluated using HPLC (high performance liquid chromatography).	Two times: (1) First day and (2) 10 weeks after assessments, adaptation and intervention
Primary	Changes in plasma irisin concentration	Collected in EDTA tubes, were centrifuged and the plasma irisin concentration was evaluated using Multiplex - Kit Human Myokine Magnetic Bead Panel.	Two times: (1) First day and (2) 10 weeks after assessments, adaptation and intervention
Primary	Changes Food intake	Food registry of 3 days. The quantification of the daily intake of nutrients will still be made using sofware.	Two times: (1) First day and (2) 10 weeks after assessments, adaptation and intervention
Primary	Changes in determination of lactate	Blood samples were collected by manual puncture of the earlobe in previously calibrated and heparinized capillary tubes, stored in eppendorf with sodium fluoride. Analyzed by electrochemical lactate analyser.	Two times: (1) First day and (2) 10 weeks after assessments, adaptation and intervention
Primary	Changes Physical Performance	Maximum effort test, according to Wilder, Brennan e Schotte (1993).	Two times: (1) First day and (2) 10 weeks after assessments, adaptation and intervention