Skeletal Muscle Protein Metabolism and Insulin Sensitivity in Overweight Individuals: Effects of Meals With Various Fatty Acid Compositions

Insulin Resistance Clinical Trial

Official title:

Skeletal Muscle Protein Metabolism and Insulin Sensitivity in Overweight Individuals: Effects of Meals With Various Fatty Acid Compositions

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT03146286
• Other study ID #: SCA/OF/12/15
• Status: Completed
• Phase: N/A
• Start date: August 1, 2015
• Est. completion date: December 3, 2015

Study information

• Verified date: May 2019
• Source: University of Nottingham
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The aim of the study will investigate whether impairment in the action of insulin to promote the use of glucose in skeletal muscle (insulin resistance) as a result of oral ingestion of a liquid meal rich in saturated fat is linked to reduced ability of muscles to synthesise new protein in response to dietary protein intake, which ultimately may compromise maintenance of muscle size and quality of life and whether partially replacing saturated fat in the liquid meal with omega 3 polyunsaturated FA (n3PUFA) will ameliorate these negative effects.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 8
• Est. completion date: December 3, 2015
• Est. primary completion date: December 3, 2015
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Male
• Age group: 40 Years to 60 Years

Eligibility

Inclusion Criteria:

• Male, healthy, overweight (BMI>27 kg/m2 and fat mass >30%) non-diabetic individuals.

Exclusion Criteria:

• Diabetes, smoking, cardiovascular disease

Related Conditions & MeSH terms

• Insulin Resistance
• Overweight

Intervention

Dietary Supplement:
• Saturated fat
Palm stearin
• Placebo
Water
• Fish Oil
Fish Oil, Omega 3 PUFA

Locations

Country	Name	City	State
n/a

Sponsors (1)

Lead Sponsor	Collaborator
University of Nottingham

References & Publications (3)

Jans A, Konings E, Goossens GH, Bouwman FG, Moors CC, Boekschoten MV, Afman LA, Müller M, Mariman EC, Blaak EE. PUFAs acutely affect triacylglycerol-derived skeletal muscle fatty acid uptake and increase postprandial insulin sensitivity. Am J Clin Nutr. 2012 Apr;95(4):825-36. doi: 10.3945/ajcn.111.028787. Epub 2012 Feb 15. — View Citation

Newens KJ, Thompson AK, Jackson KG, Wright J, Williams CM. DHA-rich fish oil reverses the detrimental effects of saturated fatty acids on postprandial vascular reactivity. Am J Clin Nutr. 2011 Sep;94(3):742-8. doi: 10.3945/ajcn.110.009233. Epub 2011 Aug 10. — View Citation

Nowotny B, Zahiragic L, Krog D, Nowotny PJ, Herder C, Carstensen M, Yoshimura T, Szendroedi J, Phielix E, Schadewaldt P, Schloot NC, Shulman GI, Roden M. Mechanisms underlying the onset of oral lipid-induced skeletal muscle insulin resistance in humans. Diabetes. 2013 Jul;62(7):2240-8. doi: 10.2337/db12-1179. Epub 2013 Mar 1. — View Citation

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Change in muscle protein fractional synthetic rate	Measurement of FSR at baseline and post intervention	3 hours post protein drink
Secondary	whole body postprandial insulin sensitivity	Glucose and insulin area under the curves (AUC) in response to the milk protein and glucose ingestion will be measured	Baseline and every 15mins for 7 hours
Secondary	Whole body and skeletal muscle fat oxidation	D31palmitate plasma enrichment and incorporation into skeletal muscle lipids	Baseline and every 15mins for 7 hours