Infertility Clinical Trial
Official title:
Identifying Markers of Oocyte Competence Through a Maturation Analysis
The aim of the study is to analyze the expression of genes and microRNAs potentially involved in oocyte competence in follicles of different sizes (<17mm and ≥17mm) at the time of oocyte pick-up.
In ART, one of the major obstacles encountered in some patients is the wide variation in the
follicular size at the oocyte pick-up day and consequently, the number of available mature
oocytes and the number of competent embryos for implantation. The follicular size at the day
of oocyte pick-up reflects the synchronicity of the individual follicular responsiveness to
FSH.
For successful preimplantation development of an embryo, the oocyte must contain all
necessary maternal factors. Two of these factors are specific mRNA transcripts (GDF9, PTX3,
HAS2, PTGS2, TNFAIP6) and microRNA (miR-224).
For each Cumulus Oocyte Complex, the evaluation of embryo development and morphokinetics will
be correlated with the expression and implantation data to identify a quantitative biomarker
to predict the embryo with the highest chance of implantation in patients.
Experimental Design:
1. - Collection of Cumulus Oocyte Complex (COC) from patients
2. - Separation of oocytes according to the size of the follicule they have been retrieved
from (<17mm; ≥17mm)
3. - Isolation of mRNA from both groups of cumulus cells
4. - Expression studies, qRT-PCR (GDF9, PTX3, HAS2, PTGS2, TNFAIP6)
5. - Analysis of miR-224, a negative regulator of cumulus expansion via PTX3
6. - Time-lapse culture & evaluation of morphokinetics
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