Clinical Trial Details
— Status: Completed
Administrative data
NCT number |
NCT02814695 |
Other study ID # |
HAWAA-001 |
Secondary ID |
|
Status |
Completed |
Phase |
Phase 1
|
First received |
|
Last updated |
|
Start date |
August 15, 2020 |
Est. completion date |
November 9, 2021 |
Study information
Verified date |
December 2021 |
Source |
Benha University |
Contact |
n/a |
Is FDA regulated |
No |
Health authority |
|
Study type |
Interventional
|
Clinical Trial Summary
The purpose of this study is to evaluate the efficacy of Baker's yeast extractions ,
probiotic antioxidant, in scavenging the oxidative stress status mediated damage on sperm
motility,progressive motility and vitality as well as comparing this potency to that of
Vitamin E in infertile men.
Description:
The practical part of the study took place from August 2020 to November 2021. The Study
population was made up of infertile males attending Hawaa fertility center, banha city,
Qaliubiya, Egypt.
40 study samples were collected from patients who indicated willingness to participate in the
study and have had 3 to 7 days of sexual abstinence, using the masturbation method and
ejaculated in wide mouthed plastic container as described by World Health Organization (WHO).
Samples were allowed to liquefy for 20 minutes and were examined for volume, viscosity,
spermatozoa count by Neubauer haemocytometer , oxidative stress level measurement by
Oxisperm, percentage of progressive motility and non-progressive motility and sperm vitality.
The Semen specimens were divided into ten equal fractions. First fraction was control, 0.1ml
of liquefied semen mixed with 0.1ml Ham's F10 medium and incubated at 37o C for 30 minutes.,
2nd: Vit. E, 3rd (Yeast extraction ( YE).Vitamin C was obtained from (Sigma, Germany), 3rd,
4th, 5th fractions (Vit. C. 1,2,3), 0.1ml liquefied semen was mixed with 0.1ml Ham's F10
medium supplemented with (0.02, 0.04, 0.06 mg/ml) Vitamin C respectively and incubated at 37o
C for 30 minutes.
After semen specimen treated in all fractions, they were examined and assessed for
microscopical changes, total motility, progressive motility, vitality, level of oxidative
stress (OS) were detected in all fractions 30 minutes later after addition of the
antioxidants.