Nitrate Supplementation on Motor Unit Activity

Healthy Clinical Trial

Official title:

Influence of Nitrate Supplementation on Motor Unit Activity During Recovery Following a Sustained Ischemic Contraction.

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT05993715
• Other study ID #: 5951
• Status: Completed
• Phase: N/A
• Start date: December 4, 2019
• Est. completion date: March 1, 2020

Study information

• Verified date: August 2023
• Source: Northumbria University
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

This study aimed to assess if nitrate supplementation influence motor unit (MU) activity following a sustained ischemic contraction and whether this is affected by blood flow restriction (BFR) during the recovery period. Fourteen male participants (mean ± SD, 25 ± 6 years) completed two experimental trials following 5-days of supplementation with either nitrate-rich (NIT) or nitrate-depleted (PLA) beetroot juice in a randomized, double-blinded, cross-over design. Intramuscular electromyography was used to assess MU potential (MUP) size and firing rates (MUFR) during a submaximal (25% MVC) sustained isometric contraction with BFR. These variables were also assessed during a 90 s recovery period with the first half completed with, and the second half completed without, BFR. Nitrate supplementation can expedite the recovery of MUP duration following a sustained ischemic contraction in healthy adults. These novel observations improve understanding of the effects of nitrate on the recovery of neuromuscular function post-exercise and might have implications for recovery of muscle contractile function.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 14
• Est. completion date: March 1, 2020
• Est. primary completion date: February 3, 2020
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Male
• Age group: 18 Years to 30 Years

Eligibility

Inclusion Criteria:

• Male between 18 - 30 years

• free from known respiratory insufficiency, cardiovascular disease and neuro-musculo-skeletal problems at present or in the preceding 6 months.

• having at least 5 years competitively (team) sport experience.

Exclusion Criteria:

• having cardiovascular disease, neuromuscular disease and any lower limb injury and treatment for chronic respiratory complaints.

Related Conditions & MeSH terms

• Dietary Supplements
• Healthy

Intervention

Dietary Supplement:
• Nitrate supplementation
Participants ingested 2 × 70 mL/day shots of concentrate nitrate-rich (~12.8 mmol/day nitrate) or nitrate-depleted (~0.08 mmol/day nitrite) beetroot juice (Beet It, James White Drinks Ltd., Ipswich, UK). Two shots were supplemented for 5 days; one each morning (~9 am) and one each evening (~9 pm) except for the day of the experimental trial when both shots were taken together 2.5 h before the experimental trial.
• Placebo supplementtaion
2 × 70 mL/day shots of concentrate nitrate-depleted (~0.08 mmol/day NO3-) beetroot juice. Two shots were supplemented for 5 days; one each morning (~9 am) and one each evening (~9 pm) except for the day of the experimental trial when both shots were taken together 2.5 h before the experimental trial.

Locations

Country	Name	City	State
United Kingdom	Northumbria University	Newcastle Upon Tyne

Sponsors (2)

Lead Sponsor	Collaborator
Northumbria University	Manchester Metropolitan University

Country where clinical trial is conducted

United Kingdom, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Plasma Nitrite Level	concentration [nmol]	Pre-exercise
Primary	Motor Unit Activity	Duration (ms), area (uV.ms), firing rate (Hz)	Pre-exercise, post-exercise, recovery (45 s)