Blood and Muscle Response to Cheddar Cheese in Healthy Adults

Healthy Clinical Trial

Official title:

Anabolic Effects of Cheddar Cheese Consumption in Human Skeletal Muscle

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT04660877
• Other study ID #: 110963
• Status: Completed
• Phase: N/A
• Start date: June 28, 2018
• Est. completion date: August 20, 2019

Study information

• Verified date: June 2022
• Source: University of Utah
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The goal of this project is to use a cross over design in young healthy male and female adults:

1. To determine the amino acid blood response to the acute ingestion of cheddar cheese in comparison to that of milk.

2. To determine the muscle mTORC1 response to the acute ingestion of cheddar cheese in comparison to that of milk.

Description:

Each subject will take part in two metabolic studies. Each metabolic study was designed to test the acute blood and muscle response to an ingested amount of either cheddar cheese or milk. Approximately, one month after the first experiment (Metabolic Study #1), the participant will complete the second experiment (Metabolic Study #2) which was exact in design and at the same time of day as the first study but the participant ingested the alternate food product. The morning of the metabolic studies, the participant will arrive at the clinical research center after a 10h fast. A catheter will be placed in the participants' arm for blood sampling. Next, the participant will undergo a fasted thigh skeletal muscle biopsy. Following the muscle biopsy, the participant will consume either cheddar cheese (65g) or milk (370 mL) each amounting to 20g of protein. Subsequent muscle biopsies will occur at 60 and 180min after product ingestion. Blood sampling will occur in the fasted state and periodically after ingestion of the products (up to 300min).

Recruitment information / eligibility

• Status: Completed
• Enrollment: 42
• Est. completion date: August 20, 2019
• Est. primary completion date: August 20, 2019
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 18 Years to 35 Years

Eligibility

Inclusion Criteria:

• Age between 18-35y

Exclusion Criteria:

1. History of cardiovascular disease

2. History of endocrine or metabolic disease (e.g., hypo/hyperthyroidism, diabetes)

3. History of kidney disease or failure

4. History of liver disease

5. History of respiratory disease (acute upper respiratory infection, chronic lung disease)

6. History of stroke with motor disability

7. Use of anticoagulant therapy (e.g., Coumadin, heparin) including aspirin and fish oils within 7d of the first biopsy experiment

8. Elevated systolic blood pressure >150 or a diastolic blood pressure > 100

9. Smoking

10. Recent anabolic or corticosteroids use (within 12 weeks)

11. Pregnancy as determined by a pregnancy test

12. self report of lactose intolerance

13. Any other medical condition or event considered exclusionary by the PI and faculty physician

Related Conditions & MeSH terms

• Healthy

Intervention

Dietary Supplement:
• Cheddar Cheese
20g of protein from cheddar cheese
• Milk
20g of protein from milk

Locations

Country	Name	City	State
United States	The University of Utah	Salt Lake City	Utah

Sponsors (1)

Lead Sponsor	Collaborator
University of Utah

Country where clinical trial is conducted

United States, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Response to cheese or milk ingestion	blood amino acids area under the curve in response to acute stimulation of cheese or milk consumption	Area under the curve 0-300min after ingestion
Secondary	Response to cheese or milk ingestion	muscle mTORC1 change in response to acute stimulation of cheese or milk	Change between 60min after ingestion and fasted response