Oxidative Stress as an Acute Exercise-induced Mechanism of Stem and Progenitor Cell Mobilization

Healthy Clinical Trial

— EISMO  

Official title:

Effect of Oxidative Stress on Acute Exercise-induced Mobilization and Apoptosis of Hematopoietic, Endothelial and Mesenchymal Stem and Progenitor Cells and Mature Endothelial Cells in Healthy Young Male Subjects

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT03747913
• Other study ID #: EISMO
• Status: Completed
• Phase: N/A
• Start date: May 2, 2019
• Est. completion date: October 31, 2019

Study information

• Verified date: December 2019
• Source: Swiss Federal Institute of Technology
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

It is already known from literature that exercise mobilizes stem and progenitor cells into the peripheral blood. However, the exact mechanisms thereof remain to be fully elucidated.

The investigators hypothesize that exercise-induced oxidative stress could be at least one of the responsible mechanisms and therefore want to study the exercise-induced stem and progenitor cell mobilization in a group of healthy young men when they exercise with, compared to when they exercise without antioxidative supplementation.

The primary outcome is numbers of stem and progenitor cells in the peripheral blood after an acute bout of exercise. As a secondary outcome, numbers of apoptotic mature and immature cells in the blood will be analysed.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 18
• Est. completion date: October 31, 2019
• Est. primary completion date: October 21, 2019
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Male
• Age group: 18 Years to 35 Years

Eligibility

Inclusion Criteria:

• Non-smoking

• Healthy

• Normal Body-Mass-Index (BMI): 18.5-24.9 kg·m-2

• Active (fulfill World Health Organization-guidelines for recommended levels of physical activity for adults aged 18 - 64 years)

• Willing to adhere to the study rules

Exclusion Criteria:

• Known or suspected non-compliance, drug or alcohol abuse

• Inability to follow the procedures of the study, e.g. due to language problems, psychological disorders etc. of the participant

• Previous enrolment into the current study

• Enrolment of the investigator, his/her family members, employees and other dependent persons

• Regular intake of medication

• Regular intake of antioxidant supplementations

Related Conditions & MeSH terms

• Healthy

Intervention

Dietary Supplement:
• Antioxidative supplementation
Each participant conducts two identical cycling tests, first without and a week later with antioxidative supplementation

Locations

Country	Name	City	State
Switzerland	Exercise Physiology Lab, Institute of Human Movement Sciences and Sport, ETH Zurich	Zürich	Zurich

Sponsors (1)

Lead Sponsor	Collaborator
Swiss Federal Institute of Technology

Country where clinical trial is conducted

Switzerland, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Changes in exercise-dependently mobilized stem and progenitor cell numbers in the peripheral blood with antioxidative supplementation	Numbers of hematopoietic, endothelial and mesenchymal stem and progenitor cells and mature endothelial cells in the peripheral blood will be measured after an acute bout of exercise first without and then with prior antioxidative supplementation.	On visit 2 after the exercise test without supplementation and then again on visit 3 after the exercise test with prior supplementation (1 week)
Secondary	Changes in numbers of exercise-induced apoptotic cells and oxidative stress markers in the peripheral blood with antioxidative supplementation	Numbers of apoptotic mononuclear cells, apoptotic hematopoietic, endothelial and mesenchymal stem and progenitor cells and apoptotic mature endothelial cells, as well as in oxidative stress markers in the peripheral blood will be measured after an acute bout of exercise first without and then with prior antioxidative supplementation.	On visit 2 after the exercise test without supplementation and then again on visit 3 after the exercise test with prior supplementation (1 week)