Impact of Non Digestible Carbohydrate on Production of Phenolic Acids From Strawberry Juice

Healthy Clinical Trial

Official title:

Impact of Non Digestible Carbohydrate on Production of Phenolic Acids From Strawberry Juice

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT03383809
• Other study ID #: BB/MO27724/1-1
• Status: Completed
• Phase: N/A
• Start date: April 28, 2017
• Est. completion date: July 6, 2019

Study information

• Verified date: July 2019
• Source: University of Glasgow
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

This is an acute human bioavailability study in self-reported healthy participants aged 20-70 years old. We hypothesize that combination of dietary polyphenolics and non-digestible carbohydrates (NDC) will increase the production of phenolic acids by bacteria in the human colon and these will be detected in urine. Participants will attend for three arms in a randomised order: Strawberry juice (a high polyphenol food), Inulin (NDC) or Mixture of strawberry juice and inulin.

Description:

Polyphenol rich plant foods have been associated with several health benefits but their bioavailability is generally low. The majority of plant polyphenols are poorly absorbed in the small intestine and enter the colon where the colonic microbiota metabolise them to release a range of phenolic acids, which are now thought to be the main bioactive components related to the reduction in disease risk. Very little is known about the impact of other constituents of the diet on the metabolism and bacterial catabolism of these polyphenols. The colonic microbiota are key agents in the release of the bioactive molecules from polyphenols but also ferment non-digestible carbohydrates (NDC) such as dietary fibre to short chain fatty acids. It is likely that there are key interactions in the colonic bacteria metabolism of fibre and phenolics. We hypothesize that combination of polyphenolics and non-digestible carbohydrates (NDC) will increase the urinary output of bioactive phenolic acids.

This study will enable a better understanding of how to deliver combinations of ingredients and nutrients to achieve maximum nutritional value and health benefits.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 15
• Est. completion date: July 6, 2019
• Est. primary completion date: July 6, 2019
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 20 Years to 70 Years

Eligibility

Inclusion Criteria:

Self-reported healthy adults

Exclusion Criteria:

Antibiotic use within the last 3 months, identified gastro-intestinal diseases, on prescribed medication other than the contraceptive pill, individuals who are pregnant or breastfeeding. Individuals who have been diagnosed as anaemic, as well as those who are allergic to any food, or paracetamol.

Related Conditions & MeSH terms

• Healthy

Intervention

Dietary Supplement:
• Strawberry juice with Inulin
Mixture of polyphenols and non digestible carbohydrates
• Strawberry Juice
Source of Polyphenols
• Inulin
Source of non digestible carbohydrate

Locations

Country	Name	City	State
United Kingdom	School of Medicine, Nursing and Dentistry, College of MVLS, University of Glasgow	Glasgow	Lanarkshire

Sponsors (2)

Lead Sponsor	Collaborator
University of Glasgow	Scottish Universities Environmental Research Centre

Country where clinical trial is conducted

United Kingdom, 

References & Publications (5)

Czank C, Cassidy A, Zhang Q, Morrison DJ, Preston T, Kroon PA, Botting NP, Kay CD. Human metabolism and elimination of the anthocyanin, cyanidin-3-glucoside: a (13)C-tracer study. Am J Clin Nutr. 2013 May;97(5):995-1003. doi: 10.3945/ajcn.112.049247. — View Citation

Henning SM, Wang P, Abgaryan N, Vicinanza R, de Oliveira DM, Zhang Y, Lee RP, Carpenter CL, Aronson WJ, Heber D. Phenolic acid concentrations in plasma and urine from men consuming green or black tea and potential chemopreventive properties for colon cancer. Mol Nutr Food Res. 2013 Mar;57(3):483-93. doi: 10.1002/mnfr.201200646. Epub 2013 Jan 14. — View Citation

Morrison DJ, O'Hara JP, King RF, Preston T. Quantitation of plasma 13C-galactose and 13C-glucose during exercise by liquid chromatography/isotope ratio mass spectrometry. Rapid Commun Mass Spectrom. 2011 Sep 15;25(17):2484-8. doi: 10.1002/rcm.5139. — View Citation

Roowi S, Mullen W, Edwards CA, Crozier A. Yoghurt impacts on the excretion of phenolic acids derived from colonic breakdown of orange juice flavanones in humans. Mol Nutr Food Res. 2009 May;53 Suppl 1:S68-75. doi: 10.1002/mnfr.200800287. — View Citation

Russell W, Duthie G. Plant secondary metabolites and gut health: the case for phenolic acids. Proc Nutr Soc. 2011 Aug;70(3):389-96. doi: 10.1017/S0029665111000152. — View Citation

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Phenolic acids bioavailability	Urine excretion of phenolic acids	0-24 hrs
Secondary	Urolithin bioavailability	Urine excretion of urolithins	0-24 hrs
Secondary	Glycaemic and appetite hormones measurements by ELISA	Plasma glucose, insulin and appetite hormone levels	0-8 hrs
Secondary	Mouth to caecum transit time	Breath hydrogen level measurements by Hydrogen monitor	0-8 hrs
Secondary	Gastric emptying time	Plasma paracetamol levels by acetaminophen assay kits	0-6 hrs