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Clinical Trial Details — Status: Completed

Administrative data

NCT number NCT02968888
Other study ID # Tine acute
Secondary ID
Status Completed
Phase N/A
First received November 10, 2016
Last updated April 9, 2018
Start date August 2014
Est. completion date May 2017

Study information

Verified date April 2018
Source Norwegian School of Sport Sciences
Contact n/a
Is FDA regulated No
Health authority
Study type Interventional

Clinical Trial Summary

The aim of this study is to investigate the acute anabolic effects of native whey, whey protein concentrate 80 (WPC-80) and milk after a bout of strength training in young and elderly. The investigators hypothesize that native whey will give a greater stimulation of muscle protein synthesis and intracellular anabolic signaling than WPC-80, and that WPC-80 will give a stronger stimulus than milk.


Description:

Increasing or maintaining muscle mass is of great importance for populations ranging from athletes to patients and elderly. Resistance exercise and protein ingestion are two of the most potent stimulators of muscle protein synthesis. Both the physical characteristic of proteins (e.g. different digestion rates of whey and casein) and the amino acid composition, affects the potential of a certain protein to stimulate muscle protein synthesis. Given its superior ability to rapidly increase blood leucine concentrations to high levels, whey is often considered the most potent protein source to stimulate muscle protein synthesis. Native whey protein is produced by filtration of unprocessed milk. Consequently, native whey has different characteristics than WPC-80, which is exposed to heating and acidification. Because of the direct filtration of unprocessed milk, native whey is a more intact protein compared with WPC-80. Of special interest is the higher amounts of the highly anabolic amino acid leucine in native whey.

The higher levels of leucine can be of great interest for elderly individuals as some studies in elderly has shown an anabolic resistance to the effects of protein feeding and strength training. By increasing levels of leucine one might overcome this anabolic resistance in the elderly.

The aim of this double-blinded, randomized, partial cross-over study is to compare the acute fractional protein synthesis and intracellular signaling response to a bout of strength training and intake of 20 grams of protein from either native whey, whey protein concentrate 80 or milk, in young and old individuals. Furthermore, the investigators wil investigate fractional protein breakdown, markers of protein breakdown, amino acid concentrations in blood.

The investigators hypothesize that native whey will induce a greater anabolic response than whey protein concentrate 80, and that whey protein concentrate 80 will give a stronger anabolic response than milk.


Recruitment information / eligibility

Status Completed
Enrollment 43
Est. completion date May 2017
Est. primary completion date April 2015
Accepts healthy volunteers Accepts Healthy Volunteers
Gender All
Age group 18 Years and older
Eligibility Inclusion Criteria:

- Healthy in the sense that they can conduct training and testing

- Able to understand Norwegian language written and oral

- Between 18 and 45, or above 70 years of age

Exclusion Criteria:

- Diseases or injuries contraindicating participation

- Use of dietary supplements (e.g. proteins, vitamins and creatine)

- Lactose intolerance

- Allergy to milk

- Allergy towards local anesthetics (xylocain)

Study Design


Related Conditions & MeSH terms


Intervention

Other:
Strength training

Dietary Supplement:
Milk 1%

Whey protein concentrate 80

Native whey


Locations

Country Name City State
Norway Norwegian School of Sport Sciences Oslo

Sponsors (4)

Lead Sponsor Collaborator
Norwegian School of Sport Sciences Arkansas Children's Hospital Research Institute, The Research Council of Norway, Tine

Country where clinical trial is conducted

Norway, 

Outcome

Type Measure Description Time frame Safety issue
Primary Mixed muscle fractional synthetic rate A continous infusion of a stable isotope (phe D5) is used to measure incorporation of tracer into muscle (biopsies from m. vastus lateralis) Three to one hours prior to a bout of strength training and protein consumption
Primary Mixed muscle fractional synthetic rate A continous infusion of a stable isotope (phe D5) is used to measure incorporation of tracer into muscle (biopsies from m. vastus lateralis) One to five hours after a bout of strength training and protein consumption
Primary Mixed muscle fractional synthetic rate Two boluses of tracer (phe13C6 and phe15N) was used to measure incorporation of tracer into muscle (biopsies from m. vastus lateralis) From three to five hours after a bout of strength training and protein consumption
Primary Mixed muscle fractional breakdown rate Two boluses of tracer (phe13C6 and phe15N) was used to measure the dilution of tracer in muscle (biopsies from m. vastus lateralis) From three to five hours after a bout of strength training and protein consumption
Secondary Ratio of phosphorylated to total ribosomal protein S6 kinase beta-1(P70S6K) change from baseline Biopsies from m. Vastus Lateralis was analyzed by western blot 30 min before, 1, 2.5 and 5 hours after training and protein intake
Secondary Phosphorylation of phosphorylated to total eukaryotic elongation factor 2 (eEF-2) change from baseline Biopsies from m. Vastus Lateralis was analyzed by western blot 30 min before, 1, 2.5 and 5 hours after training and protein intake
Secondary Phosphorylation of phosphorylated to total eukaryotic translation initiation factor 4E-binding protein 1 (4EBP-1) change from baseline Biopsies from m. Vastus Lateralis was analyzed by western blot 30 min before, 1, 2.5 and 5 hours after training and protein intake
Secondary Intracellular translocation of forkhead box O3 (FOXO3a) change from baseline Biopsies from m. Vastus Lateralis was analyzed by western blot 30 min before, 1, 2.5 and 5 hours after training and protein intake
Secondary Intracellular translocation of muscle RING-finger protein-1 (Murf-1) change from baseline Biopsies from m. Vastus Lateralis was analyzed by western blot 30 min before, 1, 2.5 and 5 hours after training and protein intake
Secondary Intracellular translocation of Atrogin1 change from baseline Biopsies from m. Vastus Lateralis was analyzed by western blot 30 min before, 1, 2.5 and 5 hours after training and protein intake
Secondary Ubiquitin Biopsies from m. Vastus Lateralis was analyzed by western blot 30 min before, 1, 2.5 and 5 hours after training and protein intake
Secondary Plasma amino acid concentration 180 and 60 min before, and 45, 60, 75, 120, 160, 180, 200, 220 and 300 min after training and protein intake
Secondary Muscle force generating capacity change from baseline Measured as unilateral isometric knee extension force (Nm) with 90° in the hip and knee joints. 15 min before, 15 and 300 min after, and 24 hours after training and protein intake
Secondary Plasma glucose 180 and 60 min before, and 45, 60, 75, 120, 160, 180, 200, 220 and 300 min after training and protein intake
Secondary Plasma insulin 180 and 60 min before, and 45, 60, 75, 120, 160, 180, 200, 220 and 300 min after training and protein intake
Secondary Serum urea 180 and 60 min before, and 60, 10, 180 and 300 min after training and protein intake
Secondary Serum ureic acid 180 and 60 min before, and 60, 10, 180 and 300 min after training and protein intake
Secondary Serum creatine kinase 180 and 60 min before, and 60, 10, 180 and 300 min after training and protein intake
Secondary Change in ATP-binding cassette transporter (ABCA1) messenger ribonucleic acid (mRNA) 1 hour after training and protein intake
Secondary Change in ABCA1 mRNA 5 hous after training and protein intake
Secondary Change in BRCA1-A complex subunit Abraxas (ABRA1) mRNA 1 hour after training and protein intake
Secondary Change in ABRA1 mRNA 5 hours after training and protein intake
Secondary Change in alfa-actin (ACTA1) mRNA 1 hour after training and protein intake
Secondary Change in ACTA1 mRNA 5 hours after training and protein intake
Secondary Change in C-C motif chemokine 2 (CCL2) mRNA 1 hour after training and protein intake
Secondary Change in CCL2 mRNA 5 hours after training and protein intake
Secondary Change in C-C motif chemokine 3 (CCL3) mRNA 1 hour after training and protein intake
Secondary Change in CCL3 mRNA 5 hours after training and protein intake
Secondary Change in C-C motif chemokine 5 (CCL5) mRNA 1 hour after training and protein intake
Secondary Change in CCL5 mRNA 5 hours after training and protein intake
Secondary Change in C-C motif chemokine 8 (CCL8) mRNA 1 hour after training and protein intake
Secondary Change in CCL8 mRNA 5 hours after training and protein intake
Secondary Change in platelet glycoprotein 4 (CD36) mRNA 1 hour after training and protein intake
Secondary Change in CD36 mRNA 5 hours after training and protein intake
Secondary Change in cholesterol 25-hydroxylase (CH25H) mRNA 1 hour after training and protein intake
Secondary Change in CH25H mRNA 5 hours after training and protein intake
Secondary Change in granulocyte colony-stimulating factor (CSF3) mRNA 1 hour after training and protein intake
Secondary Change in CSF3 mRNA 5 hours after training and protein intake
Secondary Change in C-X-C motif chemokine 16 (CXCL16) mRNA 1 hour after training and protein intake
Secondary Change in CXCL16 mRNA 5 hours after training and protein intake
Secondary Change in F-box only protein 32 (FBXO32) mRNA 1 hour after training and protein intake
Secondary Change in FBXO32 mRNA 5 hours after training and protein intake
Secondary Change in growth-regulated alpha protein (CXCL1) mRNA 1 hour after training and protein intake
Secondary Change in CXCL1 mRNA 5 hours after training and protein intake
Secondary Change in matrix metalloproteinase-9 (MMP9) mRNA 1 hour after training and protein intake
Secondary Change in MMP9 mRNA 5 hours after training and protein intake
Secondary Change in forkhead box protein O1 (FOXO1) mRNA 1 hour after training and protein intake
Secondary Change in FOXO1 mRNA 5 hours after training and protein intake
Secondary Change in forkhead box protein O3 (FOXO3A) mRNA 1 hour after training and protein intake
Secondary Change in FOXO3A mRNA 5 hours after training and protein intake
Secondary Change in hepatocyte growth factor (HGF) mRNA 1 hour after training and protein intake
Secondary Change in HGF mRNA 5 hours after training and protein intake
Secondary Change in insulin-like growth factor I (IGF1) mRNA 1 hour after training and protein intake
Secondary Change in IGF1 mRNA 5 hours after training and protein intake
Secondary Change in interleukin-10 (IL10) mRNA 1 hour after training and protein intake
Secondary Change in IL10 mRNA 5 hours after training and protein intake
Secondary Change in interleukin-17D (IL17D) mRNA 1 hour after training and protein intake
Secondary Change in IL17D mRNA 5 hours after training and protein intake
Secondary Change in interleukin-1B (IL1B) mRNA 1 hour after training and protein intake
Secondary Change in IL1B mRNA 5 hours after training and protein intake
Secondary Change in interleukin-1 receptor antagonist protein (IL1RN) mRNA 1 hour after training and protein intake
Secondary Change in IL1RN mRNA 5 hours after training and protein intake
Secondary Change in interleukin-6 (IL6) mRNA 1 hour after training and protein intake
Secondary Change in IL6 mRNA 5 hours after training and protein intake
Secondary Change in interleukin-8 (IL8) mRNA 1 hour after training and protein intake
Secondary Change in IL8 mRNA 5 hours after training and protein intake
Secondary Change in transcription factor jun-B (JUNB) mRNA 1 hour after training and protein intake
Secondary Change in JUNB mRNA 5 hours after training and protein intake
Secondary Change in kit ligand (KITLG) mRNA 1 hour after training and protein intake
Secondary Change in KITLG mRNA 5 hours after training and protein intake
Secondary Change in myostatin (MSTN) mRNA 1 hour after training and protein intake
Secondary Change in MSTN mRNA 5 hours after training and protein intake
Secondary Change in myosin-1 (MYH1) mRNA 1 hour after training and protein intake
Secondary Change in MYH1 mRNA 5 hours after training and protein intake
Secondary Change in myosin-2 (MYH2) mRNA 1 hour after training and protein intake
Secondary Change in MYH2 mRNA 5 hours after training and protein intake
Secondary Change in myosin-7 (MYH7) mRNA 1 hour after training and protein intake
Secondary Change in MYH7 mRNA 5 hours after training and protein intake
Secondary Change in oxysterols receptor LXR-alpha (NR1H3) mRNA 1 hour after training and protein intake
Secondary Change in NR1H3 mRNA 5 hours after training and protein intake
Secondary Change in nuclear receptor subfamily 4 group A member 3 (NR4A3) mRNA 1 hour after training and protein intake
Secondary Change in NR4A3 mRNA 5 hours after training and protein intake
Secondary Change in peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) mRNA 1 hour after training and protein intake
Secondary Change in PPARGC1A mRNA 5 hours after training and protein intake
Secondary Change in prostaglandin G/H synthase 2 (PTGS2) mRNA 1 hour after training and protein intake
Secondary Change in PTGS2 mRNA 5 hours after training and protein intake
Secondary Change in proton-coupled amino acid transporter 1 (SLC36A1) mRNA 1 hour after training and protein intake
Secondary Change in SLC36A1 mRNA 5 hours after training and protein intake
Secondary Change in sodium-coupled neutral amino acid transporter 2 (SLC38A2) mRNA 1 hour after training and protein intake
Secondary Change in SLC38A2 mRNA 5 hours after training and protein intake
Secondary Change in 4F2 cell-surface antigen heavy chain (SLC3A2) mRNA 1 hour after training and protein intake
Secondary Change in SLC3A2 mRNA 5 hours after training and protein intake
Secondary Change in large neutral amino acids transporter small subunit 1 (SLC7A5) mRNA 1 hour after training and protein intake
Secondary Change in SLC7A5 mRNA 5 hours after training and protein intake
Secondary Change in toll-like receptor 2 (TLR2) mRNA 1 hour after training and protein intake
Secondary Change in TLR2 mRNA 5 hours after training and protein intake
Secondary Change in tumor necrosis factor (TNF) mRNA 1 hour after training and protein intake
Secondary Change in TNF mRNA 5 hours after training and protein intake
Secondary Change in E3 ubiquitin-protein ligase TRIM63 (TRIM63) mRNA 1 hour after training and protein intake
Secondary Change in E3 ubiquitin-protein ligase TRIM63 (TRIM63) mRNA 5 hours after training and protein intake
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