Effect of Non-essential Amino Acids on Protein Requirements for Endurance Athletes

Healthy Clinical Trial

— EA3  

Official title:

Effect of Non-essential Amino Acids on Protein Requirements for Endurance Athletes Using Indicator Amino Acid Oxidation Method.

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT02874638
• Other study ID #: IAAO-EA3
• Status: Completed
• Phase: N/A
• Start date: September 2016
• Est. completion date: December 31, 2017

Study information

• Verified date: March 2019
• Source: University of Toronto
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

Protein requirements in individuals who participate in endurance-based exercise training have been suggested to be greater than the current recommended dietary allowance (RDA).

The biological value of protein depends on its amino acid composition. Essential amino acids are believed to have main role on whole body protein metabolism. However, it remains to be determined what role non-essential amino acids (NEAA) may have in regulating protein metabolism and contributing to the increased protein requirements after endurance exercise.

The indicator amino acid oxidation (IAAO) method has clarified the individual amino acid requirement in children, normal healthy adult and clinical populations. However, the IAAO method has never been utilized for assessing the effect of NEAA on protein requirements after endurance exercise.

The objective of the current study is to investigate the importance of NEAA for endurance-trained male subjects.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 7
• Est. completion date: December 31, 2017
• Est. primary completion date: August 2017
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Male
• Age group: 18 Years to 40 Years

Eligibility

Inclusion Criteria:

• endurance-trained participants who regularly run more than 40 km/week

• Participants who are categorized at least "very good" based on a study by Shvartz & Reibold, in which VO2peak is used as an index. (i.e. the participants whose VO2peak is =57 ml/kg/min (18-24 y), =54 ml/kg/min (25-29 y), =52 ml/kg/min (30-34 y), =49 ml/kg/min (35-39 y)

• Ability to complete the 16-km familiarization run in session 2

Exclusion Criteria:

• Inability to meet health and physical activity guidelines according to the physical activity readiness questionnaire (PAR-Q+)

• Inability to adhere to any of the protocol guidelines (i.e. alcohol, caffeine consumption)

• Regular tobacco use

• Illicit drug use

Related Conditions & MeSH terms

• Healthy
• Increased Metabolic Requirement

Intervention

Dietary Supplement:
• BASE egg protein
0.8 g/kg/d of protein provided as crystalline amino acid modeled after egg protein
• BCAA-enriched egg protein
BCAA-enriched egg protein provided as crystalline amino acid.
• small amount of essential amino acids
essential amino acids modeled after egg protein, provided as crystalline amino acid, which is equivalent to the amount of essential amino acid in BASE egg protein
• large amount of essential amino acids
essential amino acids modeled after egg protein, provided as crystalline amino acid, which is equivalent to the amount of amino acid in BCAA-enriched egg protein

Locations

Country	Name	City	State
n/a

Sponsors (2)

Lead Sponsor	Collaborator
University of Toronto	Ajinomoto Co., Inc.

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	13CO2 (carbon dioxide) excretion rate (µmol/kg/h)	8 hours after exercise followed by ingesting 13C-labelled phenylalanine and one of 5 different amount of amino acids intake, 13CO2 excretion rate is determined by multiplying the enrichment of 13CO2 in breath measured by Mass spectrometry and CO2 production rate measured by metabolic cart.	at 8 hours after the end of exercise]
Secondary	[13C]phenylalanine oxidation rate (µmol/kg/h)	8 hours after exercise followed by ingesting 13C-labelled phenylalanine and one of 5 different amount of amino acids intake, 13CO2 excretion rate is determined dividing 13CO2 excretion rate by urinary enrichment of 13C-phenylalanine in breath measured by Mass spectrometry, and CO2 production rate measured by metabolic cart.	at 8 hours after the end of exercise