Exercise-induced Changes in DNA-methylation

Healthy Clinical Trial

Official title:

The Effect of Exercise on Maximal Aerobic Capacity, DNA-methylation and Gene Expression in Healthy Elderly Men

Clinical Trial Details — Status: Terminated

Administrative data

• NCT number: NCT01931072
• Other study ID #: EPI-GEN100
• Status: Terminated
• Phase: N/A
• Start date: August 2012
• Est. completion date: December 2013

Study information

• Verified date: May 2019
• Source: Norwegian University of Science and Technology
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The purpose of the study was to investigate whether regular moderate- or high-intensity interval training can change the DNA methylation in a long-lasting state, and whether these changes affect the gene expression. In addition, the investigators will determine whether the intensity of the training could be an affecting factor of the possible DNA-methylation change as well. The null hypothesis was that no such exercise-induced changes occur.

Recruitment information / eligibility

• Status: Terminated
• Enrollment: 37
• Est. completion date: December 2013
• Est. primary completion date: December 2012
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Male
• Age group: 70 Years to 75 Years

Eligibility

Inclusion Criteria:

• Male

• 70-75 years old

• Signed written informed consent

Exclusion Criteria:

• Incompatible with exercise

• Known cardiac disease

• Active cancer

• Significant pulmonary disease

• Uncontrolled hypertension

• Use of blood thinning medicine (except Albyl-E)

• Drug abuse

• Alcohol abuse

• Finishing less than 80% of the exercise sessions (this complies to the training groups)

Related Conditions & MeSH terms

• Healthy

Intervention

Behavioral:
• High-intensity interval training
Interval training 3 times a week for 8 weeks
• Moderate training
Moderate training 3 times a week for 8 weeks

Locations

Country	Name	City	State
Norway	Institute for Circulation and Medical Imaging, NTNU	Trondheim

Sponsors (2)

Lead Sponsor	Collaborator
Norwegian University of Science and Technology	St. Olavs Hospital

Country where clinical trial is conducted

Norway, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	DNA-methylation	From the skeletal muscle biopsies RNA and DNA was isolated, DNA was further bi-sulfite converted and DNA-methylation was analyzed using the Illumina HumanMethylation450 BeadChip. The RNA was further used for gene expression analyses by real-time RTqPCR.	8 weeks
Primary	Gene Expression		8 weeks
Secondary	Maximal Aerobic Capacity	measured on treadmill at baseline and after eight weeks, also biopsies from Vastus lateralis were sampled.	8 weeks