Arginine Synthesis in Healthy Adult Humans

Healthy Clinical Trial

Official title:

Arginine Synthesis in Healthy Adult Humans: Does Proline or Glutamate Provide the 5-Carbon Chain?

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT01912235
• Other study ID #: 02/02/2010
• Status: Completed
• Phase: N/A
• First received: July 26, 2013
• Last updated: January 5, 2018
• Start date: September 2007
• Est. completion date: June 2008

Study information

• Verified date: January 2018
• Source: The Hospital for Sick Children
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

Arginine synthesis in mammals is a complex process involving at least 8 enzymes and intracellular and interorgan transfer of substrates.

The investigators have shown in our neonatal studies that there is no conversion of glutamate to arginine but proline label appears in ornithine, citrulline and arginine. It is clear therefore that arginine is synthesized in human neonates from proline.

In adults the source of the carbon backbone is less clear.

Description:

Through a series of test in healthy adults using tandem mass spectrometry with carbon labeled GLN and nitrogen labeled PRO to determine the dietary source for the carbon chain of ORN, CIT and ARG. Additionally, using Nitrogen labeled GLN, we will identify the source of the nitrogen atoms in these amino acids.

Using guanidino - 15N2 arginine, 1-13C Glutamine, 2-15N Glutamine and 15N Proline we will be able to:

1. determine the flux of arginine and proline in healthy adult volunteers

2. determine and compare the flux of carbon and nitrogen labeled glutamine

3. demonstrate whether glutamine or proline provides the carbon backbone for ornithine, citrulline and arginine in adults.

4. the source of the N atoms in ornithine, citrulline and arginine.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 6
• Est. completion date: June 2008
• Est. primary completion date: June 2008
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Male
• Age group: 18 Years to 55 Years

Eligibility

Inclusion Criteria:

• 5 men of average weight 80 kg will be studied

• healthy, with no know disease of liver, and/or kidney

• weight stable

Exclusion Criteria:

• weith loss or gain over the last three months

• on medications known to affect amino acid metabolism

• known liver, kidney disease condition

Related Conditions & MeSH terms

• Healthy

Intervention

Other:
• 2-15N glutamine
Glutamine with N15 isotope tracer
• 15N2 arginine and 15N proline
Arginine and Proline isotope tracers
• 1-13C glutamine
Glutamine carbon isotope tracer

Locations

Country	Name	City	State
Canada	The Hospital for Sick Children	Toronto	Ontario

Sponsors (1)

Lead Sponsor	Collaborator
The Hospital for Sick Children

Country where clinical trial is conducted

Canada, 

References & Publications (1)

Tomlinson C, Rafii M, Ball RO, Pencharz PB. Arginine can be synthesized from enteral proline in healthy adult humans. J Nutr. 2011 Aug;141(8):1432-6. doi: 10.3945/jn.110.137224. Epub 2011 Jun 15. — View Citation

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Blood Isotope Levels		8 hours

External Links

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