Elite Adolescent Athletes Clinical Trial
— ProtmorpheusOfficial title:
Effect of the Quality of Dietary Proteins on the Sleep Young Elite Athletes and the Obese Adolescent
Adolescence is characterized by major transitions in sleep and circadian rhythm. This rapid
pivotal period increases the risks of sleep debt and poor sleep quality, leading to
pronounced diurnal fatigue and drowsiness . Some adolescent's populations are more vulnerable
than others to poor sleep. Obesity and elite sports involvement are two factors that have
been distinctly associated with disturbed sleep. Several studies show that obesity was
associated with a decrease in sleep efficiency and increased arousals . On the other hand,
recent studies point out that sleep problem is widespread in young athletes which
substantially increase the risks of injury, burnout and concussions .
Despite the importance of sleep in holistic development, physical (i.e. recovery, metabolism,
muscle growth, weight control), cognitive (i.e. learning, memory, decision-making, Vigilance)
and athletic performances (Fullagar et al., 2015).
A wide range of recent papers emphasize that some nutrients take part in the regulation of
internal clock and sleep quality. The effect of tryptophan (Trp) on sleep was lifted on the
basis of the serotonergic hypothesis. Serotonin (5-HT) is synthesized from Trp circulating in
the brain following two-step procedure in raphe neurons. Serotonin, in turn, is a precursor
of melatonin and both molecules contribute to the regulation of sleep-wake behaviors.
However, Trp is an essential amino acid, which means that it cannot be synthesized by the
organism, and must exclusively be provided via degradation of proteins from the diet. The
passage of tryptophan to the brain is assured through carrier disposed at the blood-brain
barrier level. However, carrier transport depends on other competitive amino acids (LNAAs:
leucine, isoleucine, valine, phenylalanine, and tyrosine). Therefore, increased brain uptake
of tryptophan does not depend only on Trp concentrations but rather on the blood Trp / LNAA
ratio.
PROTMORPHEUS trial, was designed to examine how supplementation with proteins of different
Trp/LNAA ratio affect sleep on adolescents (obese, athletes) with mild sleep disturbances.
| Status | Recruiting |
| Enrollment | 48 |
| Est. completion date | March 1, 2022 |
| Est. primary completion date | March 1, 2021 |
| Accepts healthy volunteers | No |
| Gender | Male |
| Age group | 14 Years to 18 Years |
| Eligibility |
Inclusion Criteria: - young athletes OR adolescent with BMI > 90 percentiles Exclusion Criteria: - Disorders and / or pathology of sleep and chronic diseases of the digestive tract - Medical or surgical history not compatible with the study, any other chronic illness or injury that may interfere with the subject's abilities - Take medication that may interfere with the results of the study (corticosteroids ...) or sleeping pills - Surgical intervention in the previous 3 months - Regular consumption of tobacco, cannabis or alcohol - Special diet |
| Country | Name | City | State |
|---|---|---|---|
| France | CHU de Clermont-Ferrand | Clermont-Ferrand |
| Lead Sponsor | Collaborator |
|---|---|
| University Hospital, Clermont-Ferrand |
France,
| Type | Measure | Description | Time frame | Safety issue |
|---|---|---|---|---|
| Primary | Change effect of a supplementation with proteins of different Trp/Lnaa ratio on sleep of elite adolescent athletes with mild sleep disturbances | Efficiency of sleep (ratio of time spent in bed / sleep time) | Change from pre-intervention | |
| Secondary | change in biochemical marker related to sleep | measured by blood test : GH, GHRH, TSH, Sérotonine (5HT), Kynurénine, Tryptophane | day4 day7 | |
| Secondary | Change in biochemical markers related food intake | measured by blood test : Insuline, Leptine, Ghreline, Adiponectine, GLP-1, PYY3-36, Nesfatine, Irisine | day4 day7 | |
| Secondary | change in biochemical markers related to inflammation | measured blood test : CRP-us, IL1b, IL6, TNF-alpha, IL4, IL10, sTNFR1 and sTNFR2 | day4 day7 | |
| Secondary | Resting metabolic rate kcal per day | Resting metabolic rate. Resting metabolic rate (RMR) will be measured under fasted state, using indirect calorimetry (MetaMax 3b, Cortex Biophysik, Leipzig, Germany). In agreement with the manufacturer's recommendations gas analysis will be calibrated before the test. The test will be held in a thermoneutral environment (22-25 °C). Participants will extend in a supine position for 45 min before starting the measurements. After achieving a steady state, O2 consumption and CO2 production standardized for temperature, barometric pressure and humidity will be recorded at 1 min intervals for 20-45 min and will be averaged over the whole measurement period. After that, RMR (in kcal/day) and respiratory quotient (ratio of CO2/O2) will be calculated. | day 7 | |
| Secondary | muscular fatigue | force measurement : Time in second spent on 60% of MVIC (Maximal Voluntary Isometric Contraction (MVIC) of the knee flexors) | day 7 | |
| Secondary | Cognitive performances | Measures by cognitive tests : Stroop test | day 7 | |
| Secondary | Cognitive performances | Measures by cognitive tests :Trail marking test | day 7 | |
| Secondary | Cognitive performances | Measures by cognitive tests : Barrage test | day 7 | |
| Secondary | Cognitive performances | Measures by cognitive tests : PASAT | day 7 | |
| Secondary | Cognitive performances | Measures by cognitive tests : Symbol digit test | day 7 | |
| Secondary | Energy intake | Energy intake and proportion of the energy derived from each class of macronutrient (carbohydrate, fat, and protein) measured on meals offered "ad-libitum" | day 7 |