Elite Adolescent Athletes Clinical Trial
Official title:
Effect of the Quality of Dietary Proteins on the Sleep Young Elite Athletes and the Obese Adolescent
Adolescence is characterized by major transitions in sleep and circadian rhythm. This rapid
pivotal period increases the risks of sleep debt and poor sleep quality, leading to
pronounced diurnal fatigue and drowsiness . Some adolescent's populations are more vulnerable
than others to poor sleep. Obesity and elite sports involvement are two factors that have
been distinctly associated with disturbed sleep. Several studies show that obesity was
associated with a decrease in sleep efficiency and increased arousals . On the other hand,
recent studies point out that sleep problem is widespread in young athletes which
substantially increase the risks of injury, burnout and concussions .
Despite the importance of sleep in holistic development, physical (i.e. recovery, metabolism,
muscle growth, weight control), cognitive (i.e. learning, memory, decision-making, Vigilance)
and athletic performances (Fullagar et al., 2015).
A wide range of recent papers emphasize that some nutrients take part in the regulation of
internal clock and sleep quality. The effect of tryptophan (Trp) on sleep was lifted on the
basis of the serotonergic hypothesis. Serotonin (5-HT) is synthesized from Trp circulating in
the brain following two-step procedure in raphe neurons. Serotonin, in turn, is a precursor
of melatonin and both molecules contribute to the regulation of sleep-wake behaviors.
However, Trp is an essential amino acid, which means that it cannot be synthesized by the
organism, and must exclusively be provided via degradation of proteins from the diet. The
passage of tryptophan to the brain is assured through carrier disposed at the blood-brain
barrier level. However, carrier transport depends on other competitive amino acids (LNAAs:
leucine, isoleucine, valine, phenylalanine, and tyrosine). Therefore, increased brain uptake
of tryptophan does not depend only on Trp concentrations but rather on the blood Trp / LNAA
ratio.
PROTMORPHEUS trial, was designed to examine how supplementation with proteins of different
Trp/LNAA ratio affect sleep on adolescents (obese, athletes) with mild sleep disturbances.
Double-blind controlled pre-post trial where each subject acts as his own control.
Three measurement sessions will be performed for each teenager. Each session will be
conducted over a week. Each teenager will participate in three protein enrichment sessions:
PROT1 session, PROT2 session, and Tryptophan ratio (Trp) / neutral amino acids (AAN)
reference session (PROT REF) (PROT1: ratio = 0.07, PROT2 session: ratio = 0.11 and PROT REF
session: ratio = 0.04). During these sessions the protein intake will be doubled with regard
to recommended dietary intakes through the consumption of protein shakes (from 0.8 g per kg
of body weight to 1.6 g per kg).
At each session:
- 2 EEG sleep assessments
- 2 evaluations of the biological profile from fasting blood samples
- 2 evaluations of melatonin peak from salivary specimens
- 1 assessment of physical activity and sedentary lifestyle
- 1 evaluation of the metabolic response to submaximal exercise
- 1 assessment of muscle strength and fatigue
- 1 assessment of cognitive performance Control of food intake (3 days at RDI + 3 days
with doubled protein intake) will be achieved by preparing meals and weighing food. An
evaluation of the ad libitum food intake of the 7th day will be carried out by the
weighing method.
1. peripheral quantitative computed tomography (pQCT) of the muscular cross-section
(thigh and forearm) at the beginning of the study
;