MSC and MC in Type 2 Diabetes Mellitus

Diabetes Mellitus, Type 2 Clinical Trial

Official title:

Autologous Bone Marrow Mesenchymal Stem Cell and Bone Marrow Mononuclear Cell Infusion in Type 2 Diabetes Mellitus

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT01719640
• Other study ID #: MSC-MC-DM
• Status: Completed
• Phase: Phase 1/Phase 2
• Start date: January 2011
• Est. completion date: January 2020

Study information

• Verified date: January 2023
• Source: Fuzhou General Hospital
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

Cell injury in human islets induced by non-immune mediated inflammation occur in vitro upon hyperglycemia in type 2 diabetes mellitus. Infusion of autologous bone marrow mononuclear cells (MCs) is an emerging therapeutic approach for DM, which showed promising outcomes with mild side effects. Infusion of MCs and autologous bone marrow mesenchymal stem cells in combination might exert enhanced repairing effects. We hypothesized that infusion of these two classes of cells might provide multiple signals for regeneration and improve recovery from inflammation-induced lesion. The effects might be maximized by intra-arterial pancreatic infusion.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 22
• Est. completion date: January 2020
• Est. primary completion date: January 2013
• Accepts healthy volunteers: No
• Gender: All
• Age group: 40 Years to 65 Years

Eligibility

Inclusion Criteria:

• Ability to provide written informed consent.

• Mentally stable and able to comply with the procedures of the study protocol.

• Clinical history compatible with type 2 diabetes (T2DM) as defined by the Expert Committee on the Diagnosis and classification of Diabetes Mellitus

• Onset of T2DM disease at = 35 years of age.

• T2DM duration = 3 and = 20 years at the time of enrollment.

• Basal C-peptide 0.3-2.0 ng/mL

• HbA1c = 7.5 and = 12% before standard medical therapy (SMT). Patients must have been treated with SMT for minimum of 4 months prior to randomization.

Insulin dose and metformin doses should be stable over the 3 months prior to randomization.

• HbA1c = 7.5 and = 9.5% at time of randomization.

• Total insulin daily dose (TDD) at time of randomization should not exceed 1.0 units/day/kg

Exclusion Criteria:

• BMI >35 kg/m2.

• Insulin requirements of > 100 U/day.

• HbA1c >9.5%. (at the time of randomization)

• C-reactive protein (hs-CRP) >3.00

• Uncontrolled blood Pressure: SBP >160 mmHg or DBP >100 mmHg at the time of randomization.

• Evidence of renal dysfunction, serum creatinine > 1.5 mg/dl (males) and 1.4 mg/dl (females).

• Proteinuria > 300 mg/day

• Evidence of cardiovascular disease, existing congestive cardiac failure on physical exam and/or acute coronary syndrome in past 6 months.

• For female participants: Positive pregnancy test, presently breast-feeding, or unwillingness to use effective contraceptive measures for the duration of the study.For male participants: intent to procreate 3 months before or after the intervention or unwillingness to use effective measures of contraception. Oral contraceptives,Norplant®, Depo-Provera®, and barrier devices with spermicide are acceptable contraceptive methods; condoms used alone are not acceptable

• Active infection including hepatitis C, HIV, or TB as determined by a positive skin test or clinical presentation, or under treatment for suspected TB. Positive tests are acceptable only if associated with a history of previous vaccination in the absence of any sign of active infection. Positive tests are otherwise not acceptable, even in the absence of any active infection at the time of evaluation

• Known active alcohol or substance abuse including cigarette/cigar smoking

• Baseline Hgb below the lower limits of normal at the local laboratory; lymphopenia (<1,000/L), neutropenia (<1,500/L), or thrombocytopenia (platelets <100,000/L).

• A history of Factor V deficiency or other coagulopathy defined by INR >1.5, PTT>40, PT >15.

• Any coagulopathy or medical condition requiring long-term anticoagulant therapy(e.g., warfarin) after transplantation (low-dose aspirin treatment is allowed) or patients with an INR >1.5.

• Acute or chronic pancreatitis.

• Symptomatic peptic ulcer disease.

• Hyperlipidemia despite medical therapy (fasting LDL cholesterol >130 mg/dl, treated or untreated; and/or fasting triglycerides > 200 mg/dl).

• Receiving treatment for a medical condition requiring chronic use of systemic steroids.

• Symptomatic cholecystolithiasis.

• Use of any investigational agents within 4 weeks of enrollment.

• Admission to hospital for any reason in the 14 days prior to enrollment (signing consent).

• Presence of active proliferative diabetic retinopathy or macular edema

• Any malignancy

• Abnormal liver function >1.5 x ULN

• Abdominal aortic aneurysm

• History of cerebro-vascular accident

• Any patient with acute or subacute decompensation from diabetes

• Any acute or chronic infectious condition that in the criteria of the investigator would be a risk for the patient.

• Subjects with hypoproteinemia, cachexia or terminal states

• Subjects with history of anorexia/bulimia

• Subjects with respiratory insufficiency

• Subjects that are being treated with any medication that could interfere with the outcome of the study such as: Sulfonylureas, Thiazolidinediones and glucagon like peptide 1 (GLP-1) analogues (Exenatide, Byetta), Pramlintide (Amylin), Dipeptidylpeptidase IV (DPP-IV) inhibitors (i.e. Sitagliptin, Januvia)

• Any medical condition that, in the opinion of the investigator, will interfere with thesafe completion of the trial.

Related Conditions & MeSH terms

• Diabetes Mellitus
• Diabetes Mellitus, Type 2

Intervention

Drug:
• infusion of MSCs
infusion of MSCs
• infusion MCs
infusion of MCs
• insulin
intensive insulin care

Locations

Country	Name	City	State
China	Fuzhou General Hospital, Xiamen Univ	Fuzhou	Fujian

Sponsors (1)

Lead Sponsor	Collaborator
Fuzhou General Hospital

Country where clinical trial is conducted

China, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	macrovascular complications		8 years
Primary	microvascular complications		8 years
Secondary	DPN	diabetes peripheral neuropathy	8 years
Secondary	MI	myocardial infarction	8 years
Secondary	angina	angina	8 years
Secondary	stroke	stroke	8 years
Secondary	amputation	amputation	8 years
Secondary	DN	diabetes nephropathy	8 years
Secondary	DRP	diabetes retinopathy	8 years
Secondary	pro-DRP	proliferative diabetes retinopathy	8 years
Secondary	C-peptide AUC	C-peptide area under the curve	1y
Secondary	insulin AUC	insulin area under the curve	1y
Secondary	HbA1c	glycated hemoglobin	1y
Secondary	FBG	fasting hemoglucose	1y
Secondary	insuline dose	exogenous insulin requirements	1y
Secondary	fasting C-p	fasting c-peptide	1y
Secondary	The incidence and severity of adverse events related to the stem cell infusion procedure		8y