Assessment of Protein Modification in Chronic Kidney Disease - Selected Clinical and Biochemical Aspects

Cardiovascular Diseases Clinical Trial

Official title: Assessment of Protein Modification in Chronic Kidney Disease - Selected Clinical and Biochemical Aspects

Status Completed

Clinical Trial Summary

The studies included the effect of chronic kidney disease advancement on the accumulation of oxidative stress markers in plasma. In patients with end-stage renal disease, the effect of replacement therapy was also assessed. Therefore, the patient with chronic kidney disease was evaluated divided into three groups (chronic kidney disease at stage G3b-G4, peritoneal dialysis, hemodialysis). In addition, changes in the interrelationship between oxidative modifications, carbonyl and nitrogen stress, and the carbamylation resulting from the progression of kidney disease have been taken into account. This issue is related to the assessment of whether the protein modification types differentiate patients depending on the stage of chronic kidney disease and the method of renal replacement therapy. Protein modifications associated with oxidative stress are a part of the complications resulting from chronic kidney diseases, such as malnutrition, chronic inflammation, dyslipidemia, iron disorder, and calcium and phosphate disorders. Also, diseases of atherosclerosis aetiology are much higher frequency in patients with chronic kidney disease than in those with normal kidney function. Therefore, in the studies presented here, particular attention was paid to the effect of oxidative stress on chronic kidney disease complications in the aspect of cardiovascular damage. The specificity of atherosclerosis in patients with chronic kidney disease was evaluated by comparing groups of this type of patients with patients with ischemic heart diseases and normal renal function.

Clinical Trial Description

Redox imbalance in the course of CKD results in the intensification of oxidative and carbonyl stress, which leads to the modification of many molecules, including proteins necessary for the proper functioning of the body. The assessment of the accumulation of modified proteins in the plasma is not only an indirect indicator of the severity of redox imbalance in the system, but also allows the analysis of the influence of oxidative stress and its derivatives (glycation, carbonyl stress and carbamylation) on the pathogenesis of CKD. In addition, compounds formed as a result of the action of ROS on proteins may affect the development of long-term consequences of CKD, such as chronic inflammation, dyslipidemia, renal osteodystrophy, iron metabolism disorders and malnutrition. On the other hand, complications in patients with CKD may influence the intensification of oxidative modifications of proteins.

The following goals were set in the study:

1. Assessment of the impact of CKD advancement on the severity of protein modification as a result of oxidative stress.

2. Comparison of the effect of renal replacement therapy on protein modifications.

3. Assessment of the relationship between selected protein modifications in CKD and complications typical of CKD

4. Comparison of selected protein modifications in patients with CKD and patients with at least one history of a cardiovascular event. ;

Related Conditions & MeSH terms

• Cardiovascular Diseases
• Chronic Kidney Diseases
• Dialysis; Complications
• Kidney Diseases
• Renal Insufficiency, Chronic

• NCT number: NCT04939870
• Study type: Observational
• Source: Poznan University of Medical Sciences
• Status: Completed
• Start date: January 1, 2015
• Completion date: January 10, 2019