Ultrastructure of Atrial Myocytes in Patients in Sinus Rhythm and With Atrial Fibrillation

Atrial Fibrillation Clinical Trial

Official title:

Ultrastructure of Sarcolemma, Immunofluorescence of Calcium Handling Proteins and Mitochondrial Function in Atrial Myocytes of Patients in Sinus Rhythm and With Atrial Fibrillation

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT01493128
• Other study ID #: ABJ01
• Status: Completed
• Phase: N/A
• First received: December 13, 2011
• Last updated: March 10, 2017
• Start date: April 2010
• Est. completion date: July 2012

Study information

• Verified date: March 2017
• Source: Norwegian University of Science and Technology
• Contact: n/a
• Is FDA regulated: No
• Study type: Observational

Clinical Trial Summary

The aim of this work is to study the prevalence of T-tubular system in left and right myocytes from patients in sinus rhythm and whether this is altered in paroxysmal and permanent atrial fibrillation. Furthermore, since current information is lacking on the key Ca2+-handling proteins RyR, NCX, and SERCA-2a from patients in Atrial Fibrillation (AF), it will be investigated whether a regulation of these proteins could be an underlying cause of the disease. Mitochondrial function will also studied.

1. The investigators hypothesize that both structure of sarcolemma and localization of key Ca2+-handling proteins are altered in patients with atrial fibrillation compared with patients in sinus rhythm.

2. The investigators hypothesize that mitochondrial function is altered in patients with atrial fibrillation compared with patients in sinus rhythm.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 45
• Est. completion date: July 2012
• Est. primary completion date: February 2012
• Accepts healthy volunteers: No
• Gender: All
• Age group: 50 Years to 85 Years

Eligibility

Inclusion Criteria:

• sinus rhythm, permanent atrial fibrillation or paroxysmal atrial fibrillation

• selected for open heart surgery

• informed consent

Exclusion Criteria:

• not given informed consent

Related Conditions & MeSH terms

• Atrial Fibrillation

Locations

Country	Name	City	State
n/a

Sponsors (1)

Lead Sponsor	Collaborator
Norwegian University of Science and Technology

References & Publications (1)

Slagsvold KH, Johnsen AB, Rognmo O, Høydal MA, Wisløff U, Wahba A. Mitochondrial respiration and microRNA expression in right and left atrium of patients with atrial fibrillation. Physiol Genomics. 2014 Jul 15;46(14):505-11. doi: 10.1152/physiolgenomics.0 — View Citation

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	structure of sarcolemma		1 hour