Changes in Alveolar Macrophage Function During Acute Lung Injury

Acute Lung Injury Clinical Trial

Official title:

Changes in Alveolar Macrophage Function During Acute Lung Injury

Clinical Trial Details — Status: Not yet recruiting

Administrative data

• NCT number: NCT02693912
• Other study ID #: wch-150718
• Status: Not yet recruiting
• Phase: N/A
• First received: February 11, 2016
• Last updated: February 26, 2016
• Start date: September 2016
• Est. completion date: August 2018

Study information

• Verified date: February 2016
• Source: West China Hospital
• Contact: Chunling Jiang
• Email: jiang_chunling[@]yahoo.com
• Is FDA regulated: No
• Health authority: China: Ethics Committee
• Study type: Observational

Clinical Trial Summary

In this study, the investigators aim to observe the change in alveolar macrophage function during acute lung injury.

Description:

Alveolar macrophages are tissue-resident or recruited cells with key functions in recognition of pathogens, initiation of host defensive ang protective inflammation,and in clearance of pathogens from the airways. However, macrophages are also responsible for efficient clearance of apoptotic neutrophils, termed efferocytosis, which is a prerequisite for the timely resolution of inflammation. In this study, the investigators aim to observe the change in alveolar macrophage function during acute lung injury.

Recruitment information / eligibility

• Status: Not yet recruiting
• Enrollment: 25
• Est. completion date: August 2018
• Est. primary completion date: December 2017
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Both
• Age group: 18 Years and older

Eligibility

Inclusion Criteria:

• Patients in ICU with diagnosed ALI under mechanical ventilation

Exclusion Criteria:

• Patients with cardiac dysfunction, using steroid drugs or other drugs can that affect efferocytosis

Study Design

Observational Model: Case Control, Time Perspective: Prospective

Related Conditions & MeSH terms

• Acute Lung Injury
• Lung Injury
• Respiratory Distress Syndrome, Adult

Intervention

Procedure:
• Bronchoscopy
Bronchoscopy will be implemented under sedation in both groups.

Locations

Country	Name	City	State
n/a

Sponsors (1)

Lead Sponsor	Collaborator
West China Hospital

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	alveolar macrophages (AMs) phagocytic index	AMs will be collected and cultured in vitro. Apoptotic neutrophils will then be cocultured with AMs for 1 hour. To measure phagocytosis of apoptotic neutrophils, AMs were further processed and phagocytosed apoptotic neutrophils were detected by confocal fluorescence microscopy as described below under Cell staining and confocal microscopy. The phagocytic index was calculated according to the following formula: phagocytic index = (total number of engulfed cells/total number of counted macrophages) × (number of macrophages containing engulfed cells/total number of counted macrophages) × 100.	7 days after admission	No
Secondary	30 days mortality		30 days after admission	No