Pilot Study of the Effect of Weight Loss on Breast Tissue and Blood Biomarkers in Women at Increased Risk for Breast Cancer

Obesity Clinical Trial

Official title:

Pilot Study of the Effect of Weight Loss on Breast Tissue and Blood Biomarkers in Women at Increased Risk for Breast Cancer

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT02681120
• Other study ID #: IUSCC-0556
• Secondary ID: 1511957605
• Status: Completed
• Start date: October 7, 2016
• Est. completion date: January 30, 2020

Study information

• Verified date: October 2021
• Source: Indiana University
• Contact: n/a
• Is FDA regulated: No
• Study type: Observational

Clinical Trial Summary

This is a pilot, non-randomized, single institution, observational study investigating the effect of dramatic weight loss secondary to bariatric surgery on biomarkers of breast cancer in tissue and blood as well as on imaging in women at elevated risk for breast cancer. Twelve months after bariatric surgery, 50% of excess weight is generally expected. Eligible women at elevated risk for breast cancer who are already planning to undergo bariatric surgery will be consented to undergo imaging (MRI and mammogram), breast tissue biopsy, and fasting blood draw prior to bariatric surgery, approximately 14 days after bariatric surgery, and approximately 1 year after bariatric surgery.

In parallel we will also be assessing 40 normal breast tissue specimens as well as blood samples from the Komen Tissue Bank (elevated risk but normal BMI) to establish a normal BMI, elevated risk control group for our study. The KTB samples will be matched for general risk of breast cancer (>20%), age, race and menopausal status.

Description:

Primary Objectives

1. The effect of bariatric surgery on Background Parenchymal Enhancement (BPE) on breast MRI through comparative analysis of images of women pre- and 1 year post-bariatric surgery.

2. The effect of bariatric surgery on breast density as seen on standard four view mammograms by qualitative BIRADS category through comparative analysis of images of women pre- and 1 year post-bariatric surgery.

Secondary Objectives

3. The impact of bariatric surgery on obesity associated immune markers in breast tissue by:

• Comparing cellular immune infiltrate components [B cells (CD 20), cytotoxic T cells (CD8), helper T cells (CD4), dendritic cells (CD11c), leukocytes (CD45), and monocytes/macrophages (CD68)] in breast tissue lobules of obese women pre-, 14 days post, and 1 year post bariatric surgery, and comparing these to normal breast tissue of lean women from the KTB

• Comparing the presence of crown like structures (CLS) in breast tissue of obese women at pre-, 14 day post-, and 1 year post - bariatric surgery, and comparing these also to normal breast tissue of lean women from the KTB.

4. The effect of bariatric surgery on aromatase expression in breast tissue of obese women pre-, 14 day post-, and 1 year post-bariatric surgery, as compared to levels in breast tissue of lean women from KTB.

5. The effects of bariatric surgery on other blood correlatives pre- and 14 day and 1 year post-bariatric surgery including: 1) PGE2, TNF alpha, IL1 beta, IL-8 and IL-10 in the inflammatory pathway; 2) adiponectin, leptin, insulin, IGF1, and IGFBP3 in the insulin pathway; and 3) estradiol, SHBG, E2, T and DHEAS in the hormonal pathway. These will also be compared to blood samples from lean women in the KTB.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 3
• Est. completion date: January 30, 2020
• Est. primary completion date: January 30, 2020
• Accepts healthy volunteers: No
• Gender: Female
• Age group: 18 Years to 75 Years

Eligibility

Inclusion Criteria

1. Patients must be post-menopausal, defined as one of the following:

1. Age over 60

2. Prior oophorectomy

3. No menstrual periods in the past 12 months without a medical procedure such as hysterectomy or uterine ablation

4. FSH at castrate levels (> 40IU/L). Patients who do not meet criteria a-c (such as those under age 60 with prior hysterectomy but ovaries remain in place) will need to have an FSH level to confirm menopausal status prior to enrollment.

2. Patients must have a BMI = 30 as calculated by the formula:

Weight in pounds / height squared x 703 = BMI

A BMI of:

1. 18.5-24.9 is considered normal;

2. 25.0-29.9 is considered overweight;

3. 30.0+ is regarded as obese.

3. Patients must be planning to undergo primary bariatric surgery (no revisions).

4. Patients must be willing to provide a core tissue biopsy at baseline and with repeat tissue collection after 2 weeks and 12 months post-bariatric surgery.

5. Patients must be aged 18-75 years at the time of informed consent.

6. Patients must be accessible for treatment, adverse event tracking and follow-up as determined by the treating physician.

7. Patient consent and authorization for the release of health information must be obtained according to local institutional guidelines.

Exclusion Criteria

1. History of any malignancy except: adequately treated non-melanoma skin cancer, curatively treated in-situ cancer of the cervix, or other solid tumors curatively treated with no evidence of disease for = 5 years. History of breast cancer is not allowed.

2. Concurrent or planned participation in randomized trials of weight loss or exercise interventions or trials targeting insulin, IGF-1 or their receptors. These interventions would interfere with the endpoints.

3. Weight greater than 500 lbs at the time of imaging.

4. Known allergy to gadolinium which is used for MRI contrast.

5. History of life threatening allergic reaction to local anesthesia (lidocaine, xylocaine).

6. For the purposes of invasive breast biopsies, women must not have received therapeutic anticoagulation in the 1 month prior to enrollment, and must have no medical reason why post-operative prophylactic anticoagulation could be held for 12 hours (i.e. atrial fibrillation, history of deep venous thrombosis).

7. Prior history of breast irradiation.

8. Use of chemopreventatives such as tamoxifen or raloxifene at any time prior to enrollment.

Related Conditions & MeSH terms

• Breast Neoplasms
• Obesity
• Weight Loss

Intervention

Procedure:
• Biopsy

• Blood draw

• Mammogram

• MRI

Locations

Country	Name	City	State
United States	Indiana University Health North Hospital	Carmel	Indiana
United States	Indiana University Health Hospital	Indianapolis	Indiana
United States	Indiana University Melvin and Bren Simon Cancer Center	Indianapolis	Indiana

Sponsors (1)

Lead Sponsor	Collaborator
Tarah J Ballinger, MD

Country where clinical trial is conducted

United States, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Change in breast density via MRI from baseline	Categorical measures will be reported numerically: 1-increased, 2-decreased, 3-no change	1 year post-bariatric surgery
Primary	Change in breast density via mammography from baseline	Categorical measures will be reported numerically: 1-increased, 2-decreased, 3-no change	1 year post-bariatric surgery
Secondary	Comparison of number of crown-like structures in obese breast tissue and normal breast tissue	Numerical measures	14 days post-bariatric surgery
Secondary	Comparison of number of crown-like structures in obese breast tissue and normal breast tissue	Numerical measures	1 year post-bariatric surgery
Secondary	Change in aromatase expression markers in obese tissue from baseline	Numerical measures	14 days post-bariatric surgery
Secondary	Comparison of aromatase expression markers in normal weight breast tissue with obese breast tissue	Numerical measures	14 days post-bariatric surgery
Secondary	Change in aromatase expression markers in obese tissue from baseline	Numerical measures	1 year post-bariatric surgery
Secondary	Comparison of aromatase expression markers in normal weight breast tissue with obese breast tissue	Numerical measures	1 year post-bariatric surgery
Secondary	Change in other correlative blood markers (inflammatory, insulin, hormonal) from baseline	Numerical measures	14 days post-bariatric surgery
Secondary	Change in other correlative blood markers (inflammatory, insulin, hormonal) from baseline	Numerical measures	1 year post-bariatric surgery