Effects of Isotonic Beetroot Supplementation on Sprint Interval Exercise Performance

Exercise Performance Clinical Trial

Official title:

The Effects of Isotonic Beetroot Supplementation on Sprint Interval Exercise Performance.

Clinical Trial Details — Status: Recruiting

Administrative data

• NCT number: NCT06349018
• Other study ID #: IRB-2023-972
• Status: Recruiting
• Phase: N/A
• Start date: February 20, 2024
• Est. completion date: February 28, 2025

Study information

• Verified date: April 2024
• Source: Nanyang Technological University
• Contact: Stephen Burns
• Phone: +65 67903354
• Email: stephen.burns[@]nie.edu.sg
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

This study aims to examine the effects of dietary nitrate supplementation via beetroot juice on sprint interval cycling exercise performance. Twenty-four participants (male or female) will undertake a randomized, crossover trial where they consume either (i) an isotonic beetroot juice drink containing nitrate (12.9 mmol) for 5 days or (ii) an isotonic sports drink (no nitrate) for 5 days. On Day 5 of each trial, participants will come to the laboratory and 2 hours after consuming their allocated drink will complete a 6 bouts of sprint interval exercise. Power output during cycling will be measured as the primary outcome measure. Salivary and plasma measures of nitrate and nitrite will be taken over the course of the morning on Day 5. After a 5-day washout period participants will crossover and begin their second trial.

Description:

This study aims to examine the effects of dietary nitrate supplementation via beetroot juice on sprint interval cycling exercise performance. Twenty-four participants (male or female) will undertake a randomized, crossover trial where they consume either (i) an isotonic beetroot juice drink containing nitrate (12.9 mmol) for 5 days or (ii) an isotonic sports drink (no nitrate) for 5 days. Drinks will be equivalent in isotonicity and colored the same so that participants and investigators will be blinded to each trial. Randomization and drink allocation will be completed by a third person not associated with the study team. On Day 5 of each trial, participants will come to the laboratory and 2 hours after consuming their allocated drink will complete a 6 bouts of sprint interval exercise (10 second all-out maximal sprints interspersed with 50 seconds rest). Power output (peak and mean) during cycling sprints will be measured as the primary outcome measure. Salivary measures of nitrate/nitrite will be taken at three time points over the course of the morning on Day 5 - (i) pre-drink consumption; (ii) 2 hours post-drink consumption; and (iii) post-exercise. Plasma measures of nitrate and nitrite will be taken at two time points over the course of the morning on Day 5 - (i) pre-drink consumption; and (ii) 2 hours post-drink consumption. Near infra-red spectroscopy (NIRS) will be used to measure muscle (vastus lateralis) oxygenation during cycling bouts. A Stroop test to measure inhibition (executive function) will be taken pre- and post-exercise. Sensory perception of the drink will be measured immediately post-consumption on Day 5. After a 5-day washout period participants will crossover and begin their second trial.

Recruitment information / eligibility

• Status: Recruiting
• Enrollment: 24
• Est. completion date: February 28, 2025
• Est. primary completion date: February 28, 2025
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 21 Years to 40 Years

Eligibility

Inclusion Criteria:

• Age: 21 - 40 years

• Healthy and injury-free (e.g., no cardiovascular disease, diabetes mellitus, orthopaedic impairment that interferes with moderate-to-vigorous exercise)

• Blood pressure < 130/90 mmHg

• Be physically active: Engaging in a minimum of 150 minutes of moderate-intensity exercise or 75 minutes of vigorous-intensity physical activity each week.

• Recreational cyclist (Minimum 1 hour cycling per week).

• Body mass index: 18.5-25 kg/m2

• Non-smoker

• Asian

Exclusion Criteria:

• Any allergies to beetroot or isotonic drink.

• Asthma

• Any diagnosed form of cardiometabolic disease (heart disease, stroke, peripheral vascular disease, diabetes, metabolic syndrome, high blood pressure).

• Any symptoms contraindicating exercise testing (e.g. chest pains).

• Any balance or dizziness problems.

• Any chronic medical conditions (whether medicated or not).

• Any bone joint problems.

• Any Physician diagnosed contraindications to exercise.

• Any other health problems that may be a cause for concern or contraindication to exercise.

• Any recent Covid vaccination (within last 2 weeks).

Related Conditions & MeSH terms

• Exercise Performance

Intervention

Other:
• Dietary Supplement: High nitrate isotonic drink
High nitrate (12.9 mmol) drink with carbohydrate and minerals
• Dietary Supplement: No nitrate isotonic drink
Carbohydrate and mineral isotonic drink

Locations

Country	Name	City	State
Singapore	Human Bioenergetics Laboratory, National Institute of Education, Nanyang Technological University	Singapore

Sponsors (1)

Lead Sponsor	Collaborator
Nanyang Technological University

Country where clinical trial is conducted

Singapore, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Power output during maximal sprint cycling	Peak and mean power output	2 hours after drink ingestion
Secondary	Salivary nitrate	Salivary nitrate/nitrite concentration	Pre-drink, 2 hours post-drink and immediately post-exercise (2.5 hours post-drink)
Secondary	Plasma nitrate and nitrite	Plasma nitrate and nitrite	Pre-drink and 2 hours post-drink
Secondary	Muscle oxygenation	Near infra-red spectroscopy	During exercise (2-2.5 hours post drink)
Secondary	Sensory evaluation (Acceptance and Preference Test Evaluation by Meilgaard et al 2007)	Drink taste and perception (7-point scale - 1 extremely dislike to 7 extremely like)	Immediately post-drink (1 minute post-drink)
Secondary	Stroop test	Reaction time and correct answers	Immediately pre- (2 hours post-drink) and immediately post-exercise (2.5 hours post drink)