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Clinical Trial Details — Status: Recruiting

Administrative data

NCT number NCT06349018
Other study ID # IRB-2023-972
Secondary ID
Status Recruiting
Phase N/A
First received
Last updated
Start date February 20, 2024
Est. completion date February 28, 2025

Study information

Verified date April 2024
Source Nanyang Technological University
Contact Stephen Burns
Phone +65 67903354
Email stephen.burns@nie.edu.sg
Is FDA regulated No
Health authority
Study type Interventional

Clinical Trial Summary

This study aims to examine the effects of dietary nitrate supplementation via beetroot juice on sprint interval cycling exercise performance. Twenty-four participants (male or female) will undertake a randomized, crossover trial where they consume either (i) an isotonic beetroot juice drink containing nitrate (12.9 mmol) for 5 days or (ii) an isotonic sports drink (no nitrate) for 5 days. On Day 5 of each trial, participants will come to the laboratory and 2 hours after consuming their allocated drink will complete a 6 bouts of sprint interval exercise. Power output during cycling will be measured as the primary outcome measure. Salivary and plasma measures of nitrate and nitrite will be taken over the course of the morning on Day 5. After a 5-day washout period participants will crossover and begin their second trial.


Description:

This study aims to examine the effects of dietary nitrate supplementation via beetroot juice on sprint interval cycling exercise performance. Twenty-four participants (male or female) will undertake a randomized, crossover trial where they consume either (i) an isotonic beetroot juice drink containing nitrate (12.9 mmol) for 5 days or (ii) an isotonic sports drink (no nitrate) for 5 days. Drinks will be equivalent in isotonicity and colored the same so that participants and investigators will be blinded to each trial. Randomization and drink allocation will be completed by a third person not associated with the study team. On Day 5 of each trial, participants will come to the laboratory and 2 hours after consuming their allocated drink will complete a 6 bouts of sprint interval exercise (10 second all-out maximal sprints interspersed with 50 seconds rest). Power output (peak and mean) during cycling sprints will be measured as the primary outcome measure. Salivary measures of nitrate/nitrite will be taken at three time points over the course of the morning on Day 5 - (i) pre-drink consumption; (ii) 2 hours post-drink consumption; and (iii) post-exercise. Plasma measures of nitrate and nitrite will be taken at two time points over the course of the morning on Day 5 - (i) pre-drink consumption; and (ii) 2 hours post-drink consumption. Near infra-red spectroscopy (NIRS) will be used to measure muscle (vastus lateralis) oxygenation during cycling bouts. A Stroop test to measure inhibition (executive function) will be taken pre- and post-exercise. Sensory perception of the drink will be measured immediately post-consumption on Day 5. After a 5-day washout period participants will crossover and begin their second trial.


Recruitment information / eligibility

Status Recruiting
Enrollment 24
Est. completion date February 28, 2025
Est. primary completion date February 28, 2025
Accepts healthy volunteers Accepts Healthy Volunteers
Gender All
Age group 21 Years to 40 Years
Eligibility Inclusion Criteria: - Age: 21 - 40 years - Healthy and injury-free (e.g., no cardiovascular disease, diabetes mellitus, orthopaedic impairment that interferes with moderate-to-vigorous exercise) - Blood pressure < 130/90 mmHg - Be physically active: Engaging in a minimum of 150 minutes of moderate-intensity exercise or 75 minutes of vigorous-intensity physical activity each week. - Recreational cyclist (Minimum 1 hour cycling per week). - Body mass index: 18.5-25 kg/m2 - Non-smoker - Asian Exclusion Criteria: - Any allergies to beetroot or isotonic drink. - Asthma - Any diagnosed form of cardiometabolic disease (heart disease, stroke, peripheral vascular disease, diabetes, metabolic syndrome, high blood pressure). - Any symptoms contraindicating exercise testing (e.g. chest pains). - Any balance or dizziness problems. - Any chronic medical conditions (whether medicated or not). - Any bone joint problems. - Any Physician diagnosed contraindications to exercise. - Any other health problems that may be a cause for concern or contraindication to exercise. - Any recent Covid vaccination (within last 2 weeks).

Study Design


Related Conditions & MeSH terms


Intervention

Other:
Dietary Supplement: High nitrate isotonic drink
High nitrate (12.9 mmol) drink with carbohydrate and minerals
Dietary Supplement: No nitrate isotonic drink
Carbohydrate and mineral isotonic drink

Locations

Country Name City State
Singapore Human Bioenergetics Laboratory, National Institute of Education, Nanyang Technological University Singapore

Sponsors (1)

Lead Sponsor Collaborator
Nanyang Technological University

Country where clinical trial is conducted

Singapore, 

Outcome

Type Measure Description Time frame Safety issue
Primary Power output during maximal sprint cycling Peak and mean power output 2 hours after drink ingestion
Secondary Salivary nitrate Salivary nitrate/nitrite concentration Pre-drink, 2 hours post-drink and immediately post-exercise (2.5 hours post-drink)
Secondary Plasma nitrate and nitrite Plasma nitrate and nitrite Pre-drink and 2 hours post-drink
Secondary Muscle oxygenation Near infra-red spectroscopy During exercise (2-2.5 hours post drink)
Secondary Sensory evaluation (Acceptance and Preference Test Evaluation by Meilgaard et al 2007) Drink taste and perception (7-point scale - 1 extremely dislike to 7 extremely like) Immediately post-drink (1 minute post-drink)
Secondary Stroop test Reaction time and correct answers Immediately pre- (2 hours post-drink) and immediately post-exercise (2.5 hours post drink)
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