Severe Combined Immunodeficiency, Atypical Clinical Trial
Official title:
Evaluation of the Clinical Utility and Cost Effectiveness Ratio of Generalized Neonatal Screening for Severe Combined Immunodeficiencies (SCID) by Quantification of TRECs on Guthrie Cards
Severe combined Immunodeficiencies ( SCID ) are a group of inherited diseases of the immune
system by characterised profound abnormalities of T cell development . Infants with SCID
require prompt clinical response to Prevent life -threatening infection and studies show
significantly improved survival in babies Diagnosed at birth as a result of previous family
history . SCID follows criteria for population -based newborn screening since it is
asymptomatic at birth and fatal within the first year of life, the confirmation of the
disease is easy, there is a curative treatment , and it is known that early stem cell
transplantation improves survival . Quantification of TRECs (T- cell receptor excision
circles ) in DNA extracted from Guthrie samples is a sensitive screening test for Specific
and SCID .
The investigators propose in this study to perform a neonatal screening of SCID , in a
population of 200,000 babies over a period of two years .
The investigators propose to study the clinical utility and cost effectiveness ratio, and
SCID screening to demonstrate that could result in a broad benefit to Individuals detected ,
making screening relatively cost-effective in spite of the low incidence of the disease .
The project proposes to study the feasibility and cost-effectiveness ratio ( time management
and life expectancy to 10 years) of generalized neonatal screening for SCID children by
offering this screening to 200 000 children (100 000 children per year) over the entire
territory. Prospective control group consists of children diagnosed with SCID out of 700,000
annual births who do not benefit from screening.
The protocol will be leant against the existing newborn screening , that is to say two more
drops of blood are placed on a second Guthrie card when current screening (72 hours of life )
is performed after parents' information and consent. Eleven newborn screening regional
associations will be involved with the inclusion of children in about 50 maternity hospitals.
The card drawn for the protocol will follow the usual network except that the test for
quantifying TRECs will be realized in two laboratories instead of eleven laboratories
assigned to RA . Investigative Regional Associations (RAs) represent nearly 600,000 births /
year and the amount of 200,000 children will be achieved in two years (duration of inclusion)
. All children born in the participating maternity may be included if they meet the inclusion
criteria. The result of the screening test for SCID will be available within 21 days after
birth, provided that there is no need to request a new sample.
At each of eleven RA is associated a pediatrician referent for immune deficiencies, member of
the french reference center (CEREDIH) and who will be responsible to call the parents, offer
them a consultation and further exploration if the result of screening is assumed positive.
Analysis of cards from 200,000 children will give the following information:
- Number of children with a presumptive positive screening , requiring a call by the
referent pediatrician, consultation and exploration of lymphocyte subpopulations
- Number of children with a negative screening
- Number of children with an inconclusive screening (lack of TRECs and lack of
amplification of the reference gene) and requiring a new card,
A micro- costing study will be conducted to assess the cost of testing .
This group of 200,000 children is the experimental group to assess the cost of screening ,
acceptability by parents (participation rate), the recall rate for abnormal or inconclusive
result, the rate of follow-up time for results , the incidence of disease . It will also
allow to calculate the specificity of the method .
At the end of the inclusions, the vital status at 18 months with cause of death will be
sought for the 200 000 children included , with the CESP ( Centre de Recherche en
Epidemiologie et Santé des Populations) via RNIPP (Répertoire National d'Identification des
Personnes Physiques) and CepiDc ( Centre d'Epidémiologie sur les causes médicales de décès) .
This will establish whether there are SCID in this population which were not detected at
birth. Furthermore, the investigators include in the study SCID children diagnosed without
screening by pediatricians local referents DIP (including Necker main transplant center) .
This will enable to approach the sensitivity of the method . All these data allow the
calculation of the predictive values of the test.
In this experimental group will be isolated a group of individuals who screened positive and
diagnosed as true SCID . Clinical data for these patients will be collected in an electronic
CRF ( CRF ) by the pediatrician referral protocol (Dr Thomas C ) , including:
- The dates and results of explorations : lymphocyte subpopulations , blood count,
determination of immunoglobulin levels
- The diagnosis made with identification of the genetic defect
- The date of care before curative treatment ( protected area isolation , anti -infective
drugs )
- , Bacterial , fungal anti -viral treatments , and other
- The date of transplant, type of transplant or other treatment ... ...
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