Study on Antigen-presenting Function of Gamma Delta T Cells in Sepsis and Its Molecular Mechanisms

Sepsis Clinical Trial

Official title:

Study on Antigen-presenting Function of Gamma Delta T Cells in Sepsis and Its Molecular Mechanisms

Clinical Trial Details — Status: Not yet recruiting

Administrative data

• NCT number: NCT03379896
• Other study ID #: 81701880
• Status: Not yet recruiting
• Phase: N/A
• First received: December 16, 2017
• Last updated: December 20, 2017
• Start date: January 4, 2018
• Est. completion date: December 30, 2020

Study information

• Verified date: December 2017
• Source: West China Hospital
• Contact: Xuelian Liao, MD
• Phone: 8613541023033
• Email: xuelianliao[@]hotmail.com
• Is FDA regulated: No
• Study type: Observational

Clinical Trial Summary

Study on antigen-presenting function of gamma delta T cells in sepsis and its molecular mechanisms

Description:

The latest definition of sepsis highlights the role of dysregulated host response to infection in organ dysfunction aggravation. Maintenance of immune homeostasis of septic patients through immunotherapy is the key breakthrough to improve success rate. Antigen-presenting cell (APC) is the bridge that connects the innate and adaptive immunity. Decrease of count and function of APC has been shown to be associated with worsened clinical outcomes in patients with sepsis. Gamma-delta T cells (γδ T cells) are the newly identified population of T lymphocytes. Recent studies have found that γδ T cells possess unique and powerful antigen-presenting function, making them the hot topic in infection and cancer research. Based on the results, the investigator plan to evaluate the antigen-presenting function changes of γδ T cells in septic status by analyzing the antigen-presenting related molecules on γδ T cells, antigen protein uptake ability, and their function on the proliferation and activation of CD8+ T lymphocytes. Furthermore, the investigator will explore the mechanism and signal pathways for the APC function changes of γδ T cells. Findings from this research could help explain the mechanism of sepsis induced immune dysfunction, enrich our understanding of the important role of γδ T cells, and build a good foundation for immunotherapy in sepsis.

Recruitment information / eligibility

• Status: Not yet recruiting
• Enrollment: 60
• Est. completion date: December 30, 2020
• Est. primary completion date: June 30, 2020
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 18 Years to 80 Years

Eligibility

Inclusion Criteria:

1. =18 year old;

2. a known or suspected infection based on clinical data at the time of admission and sepsis-induced dysfunction of at least one organ.

Exclusion Criteria:

1. autoimmune disease,

2. history of transplantation,

3. acute tuberculosis,

4. chronic hepatitis B or C infection,

5. HIV infection,

6. active malignancy, and

7. long-term use of cortical steroids.

Related Conditions & MeSH terms

• ?d T Cells
• Antigen-presenting Function
• Sepsis
• Toxemia

Intervention

Other:
• Collect peripheral blood sample
Collect 20 ml heparinized blood from patients and controls strictly abiding by the principle of aseptic manipulation after informed consent.

Locations

Country	Name	City	State
n/a

Sponsors (1)

Lead Sponsor	Collaborator
West China Hospital

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	28-day clinical outcome	death	28-day after ICU admission