Estimation of Level of ERAP1 Expression in Lesional and Non Lesional Skin of Psoraitic Patients and Healthy Controls.

Psoriasis Clinical Trial

Official title:

Estimation of Level of ERAP1 Expression in Lesional and Non Lesional Skin of Psoraitic Patients and Healthy Controls.

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT03767114
• Other study ID #: EgyERAP
• Status: Completed
• Phase: N/A
• Start date: January 2017
• Est. completion date: December 2018

Study information

• Verified date: December 2018
• Source: Kasr El Aini Hospital
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

A 4mm Punch biopsy is obtained from lesional and non lesional skin of 25 psoraitic patients as well as of 30 controls.

Skin biopsy from patients (psoriasis lesional and non lesional) & control will be kept in lysis solution (which contains RNAase inhibitor) for the stability of the parameter to be assessed. ERAP-1 gene expression will Research Template 5 Final Version: 8/3/2 016 be detected by quantitative real time -PCR, which include RNA extraction, cDNA synthesis & PCR.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 55
• Est. completion date: December 2018
• Est. primary completion date: June 2017
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 18 Years to 75 Years

Eligibility

Inclusion Criteria:

• patients with psoriasis vulgaris

• patients over 18 years of age

• both sexes

Exclusion Criteria:

• patients on treatment for psoriasis within the last two months

• assoiciated autoimmune diseases eg SLE

• pregnancy

Related Conditions & MeSH terms

• Psoriasis

Intervention

Diagnostic Test:
• RT-PCR for ERAP1 detection
Reverse transcriptase -Polymerase Chain Reaction for ERAP1 detection

Locations

Country	Name	City	State
n/a

Sponsors (1)

Lead Sponsor	Collaborator
Kasr El Aini Hospital

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Estimation of lesional ERAP1 in psoriatic skin	DPCRetection of level of expression of ERAP 1 in lesional skin of psoriasis patients by RT-	Maximum 2 years
Secondary	Estimation of non lesional ERAP1 in psoriatic skin	Detection of level of expression of ERAP 1 in non lesional skin of psoriasis patients by RT-PCR.	Maximum 2 years