Clinical Trial Summary
Anemia by the Centers for Disease Control and Prevention (CDC, 1989); It is defined as
hemoglobin (Hb) or
hematocrit (Hct) values below the 5th percentile in pregnant women. It is expressed as Hb
value of 11 mg / dl
or Hct value below 33% in the first trimester. II. Less than 10.5 g / dl in trimester can be
defined as anemia.
Anemia is a risk factor during pregnancy and one of the predisposing causes of maternal
death. Pregnancy
anemia can be aggravated by various conditions such as uterine or placental bleeding,
gastrointestinal
bleeding and peripartum blood loss. In addition to the general consequences of anemia, there
are specific risks for the mother and fetus during pregnancy such as intrauterine growth
retardation, prematurity, feto-placental miss rate, and higher risk for peripartum blood
transfusion. Literature studies on anemia in risky age groups during pregnancy (adolescence
and advanced age pregnancy) have been reviewed. Some articles and theses published in Turkey
and abroad were found. However, the number of studies related to the study subject in Turkey
is quite small. For this reason, conducting a research on the effect of anemia on the
placenta during pregnancy will lead to a decrease in the gap in the literature and an
increase in the awareness of the public on the subject when the research results are
obtained. The research is of a prospective type. The universe of the study will be composed
of pregnant women who voluntarily agree to participate in the study chosen by the improbable
sampling method. According to the anemia of the pregnant women within the scope of the
research; I: Group: Anemic pregnancies under the age of 18 (study group), II: Group: Anemic
pregnancies over the age of 35 (study group) III: Group : Pregnant women under the age of 18
are not anemic (control group) IV: Group : Non-anemic pregnant women over the age of 35
(control group) as planned. The placentas of pregnant women who gave birth between 37-42
weeks of age and who voluntarily accepted to participate in the study and who were compatible
with age and parity will be examined histopathologically, with a total of at least 30
placentas from each group. Those who had an out-of-term delivery, had multiple pregnancy,
preeclampsia, gestational diabetes mellitus, thyroid dysfunction, systemic diseases other
than anemia, and those who used drugs other than anemia during pregnancy will not be included
in the study. The research will only be conducted with women who have a normal vaginal
delivery. In our research, it will be examined whether the possible changes that anemia
(anemia) will make in the expression of IRP protein 1 produced from the placenta affect the
placenta and the baby. In addition, the relationship between IRP protein 1 and primary
related TFrP 1 and indirectly changes in DMT1 expressions will be evaluated, so that the
intrauterine growth retardation (IUGG), which is one of the negative effects of anemia in
pregnancy, and TFrP 1, are the molecules that increase the end-stage release from the
placenta. and its relationship in terms of Dmt1 molecules will be investigated. The tissue
samples of placenta that will be obtained after the experiment periods, will be buried in
paraffin-embedded blocks after routine tissue tracing, and from the obtained blocks, 5
microns of incisions will be taken via microtome, which will then be stained with
Hematoxylin-Eosin and then HPL, IGF and leptin expression levels will be examined with the
light microscope by immunohistochemical staining. The results of the research data created by
determining the effect of the histopathological examinations on the placentas of women with
anemia seen in pregnant women in risky age groups will be a source for the next studies.
Maternal anemia (of the mother) seen in pregnancies has been studied many times before, but
maternal anemia seen in adolescents and advanced age groups will add a different dimension to
the subject and will shed light on other studies on the subject. In this way, it is aimed to
draw attention to other researches to be carried out to take the necessary measures and
measures against these problems.