Impact of Sprint Stair Climbing "Snacks" on Markers of Metabolism and Vascular Function

Physical Activity Clinical Trial

Official title:

Salivary Insulin Profiles Throughout the Day in Healthy Humans

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT03374436
• Other study ID #: H17-01747
• Status: Completed
• Phase: N/A
• Start date: October 1, 2017
• Est. completion date: August 30, 2019

Study information

• Verified date: September 2019
• Source: University of British Columbia
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

Prolonged sitting has been shown to impair metabolism and vascular function. The overall purpose of this study is to determine if breaking up prolonged (9 hours) of sitting with brief (~20 second) stair climbing exercise "snacks" can improve markers of metabolic control and vascular health in healthy young male participants and in males or females with overweight/obesity who are at risk of insulin resistance. An additional purpose is to determine if saliva insulin can be used as a valid indicator of blood insulin when measured throughout the day in sedentary and active conditions and when diets with different amounts of carbohydrates are consumed.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 23
• Est. completion date: August 30, 2019
• Est. primary completion date: June 30, 2019
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 18 Years to 65 Years

Eligibility

Lean Group Inclusion Criteria:

Non-smoking

Lean Group Exclusion Criteria:

Diagnosed with diabetes or prediabetes (fasting blood sugar more than 6.0 mmol/l) or any other chronic condition that may impact glucose or insulin levels.

BMI is over 25 kg/m2 Take medications which may affect glucose and insulin levels Allergic to eggs. Smoker Competitive or serious endurance athlete.

Overweight/Obese Group Inclusion Criteria:

Elevated waist circumference (>102 cm for male and >88 cm for female)

Overweight/Obese Group Exclusion Criteria:

Diagnosed with diabetes (fasting blood sugar more than 7.0 mmol/l) Take medications which may affect glucose and insulin levels Allergic to eggs. Smoker Competitive or serious endurance athlete.

Related Conditions & MeSH terms

• Diet Modification
• Physical Activity

Intervention

Behavioral:
• High-Carbohydrate Sedentary
Three high-carbohydrate meals will be consumed (breakfast, lunch, and dinner) at ~3 hour intervals while participants remain in a seated position for the entire 9 hour intervention period.
• High-Carbohydrate Active
Three high-carbohydrate meals will be consumed (breakfast, lunch, and dinner) at ~3 hour intervals while participants remain in a seated position for the entire 9 hour intervention period except that once every hour they will ascend three flights of stairs vigorously (stair climbing sprint "snack") for a total of 8 active breaks.
• Low-carbohydrate Sedentary
Three low-carbohydrate meals will be consumed (breakfast, lunch, and dinner) at ~3 hour intervals while participants remain in a seated position for the entire 9 hour intervention period. This condition will provide a condition where the blood glucose and insulin responses are relatively low and stable throughout the day.

Locations

Country	Name	City	State
Canada	University of British Columbia Okanagan	Kelowna	British Columbia

Sponsors (3)

Lead Sponsor	Collaborator
University of British Columbia	CoreHealth Technologies Inc., Mitacs

Country where clinical trial is conducted

Canada, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Plasma insulin area under the curve	The plasma insulin concentration measured by enzyme-linked immunosorbent assay area under the curve will be measured using the trapezoidal rule.	Measured across 9 hours of each intervention day
Secondary	Saliva insulin area under the curve	The saliva insulin concentration measured by enzyme-linked immunosorbent assay area under the curve will be measured using the trapezoidal rule.	Measured across 9 hours of each intervention day
Secondary	Plasma glucose area under the curve	The plasma glucose concentration measured by hexokinase method area under the curve will be measured using the trapezoidal rule.	Measured across 9 hours of each intervention day
Secondary	Plasma triglyceride area under the curve	The plasma triglyceride concentration measured by biochemical assay area under the curve will be measured using the trapezoidal rule.	Measured across 9 hours of each intervention day
Secondary	Plasma non-esterified fatty acids area under the curve	The plasma non-esterified fatty acid concentration measured by biochemical assay area under the curve will be measured using the trapezoidal rule.	Measured across 9 hours of each intervention day
Secondary	Femoral artery flow-mediated dilation	Flow-mediated dilation of femoral artery measured by vascular ultrasound	Measured in the morning (time 0 hours) and afternoon (time 8.5 hours) each intervention day
Secondary	Cerebrovascular function	Cerebrovascular function measured by transcranial doppler ultrasound	Measured in the morning (time 0.5 hours) and afternoon (time 6 hours) each intervention day
Secondary	Cognitive function	Cognitive function measured by Computerized Neurocognitive Assessment Software (CNS) Vital Signs test battery	Measured in the morning (time 0.5 hours) and afternoon (time 6 hours) each intervention day