Clinical and Metagenomic Investigation of Antimicrobial Peptide Gel in Periodontal Treatment.

Periodontitis Clinical Trial

Official title:

Clinical and Metagenomic Investigation of Locally Delivered Synthetic Antimicrobial Peptide Gel in Non-surgical Periodontal Treatment: a Pilot Study.

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT05125718
• Other study ID #: AMP-01
• Status: Completed
• Phase: Early Phase 1
• Start date: January 1, 2021
• Est. completion date: April 30, 2021

Study information

• Verified date: November 2021
• Source: University of Malaya
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

Background and objective(s): Periodontitis is initiated by a dysbiotic host-microbe relationship. Standard periodontal treatment involves oral hygiene education, mechanical debridement and periodic follow-ups with the aims of pocket closure and maintaining a symbiotic microbial community. Nonetheless, this therapy alone may fail due to the limitations of mechanical instrumentation. The use of antimicrobial in combination to subgingival debridement has been proposed for initial and recurrent periodontitis to enhance the effectiveness of mechanical instrumentation. Antimicrobial peptide (AMP) comprised of a wide range of peptide, was found naturally in various life forms or manufactured as a synthetic compound. This study was conducted to evaluate the use of AMP as an adjunct to non-surgical periodontal treatment in terms of clinical and microbiological outcomes.

Hypothesis

• There are changes in clinical periodontal parameters and subgingival microbial profile following treatment with locally delivered synthetic AMP.

Description:

study design: This was a split-mouth randomised pilot study of a 3-months follow-up.

Sample size calculation: The sample size was calculated using G*Power software version 3.1.9.4 (Franz Faul, Universitat Kiel, Germany). Estimation of sample size was based on the mean and standard deviation of CAL between the test and control group of previous publication (Singh et al., 2018). Four samples in each group (test and control) were required to achieve 80% power at a significant level of 5%.

Randomisation: The random allocation sequence was generated by another person who was not directly involved in the study using a Research Randomizer tools (https://www.randomizer.org/). Periodontal sites of each patient were randomised at a split mouth level (left or right halves of the dentition) to one of the two treatment groups. Allocated treatments were inserted into an opaque envelop. Treatment allocation was concealed from the clinician and only revealed once subgingival debridement was completed.

intervention: At baseline, clinical measurement was recorded and target sites (sites with PPD ≥6mm) were identified. subgingival plaque were collected at target sites. The same clinician performed full mouth scaling and subgingival debridement, coupled with standardised oral hygiene instruction for all subjects. Upon the completion of treatment, second clinician applied the treatments according to the randomisation.

• Test group: Subgingival instrumentation followed by Antimcrobial peptide gel (AMP) application in all target sites.

• Control group: Subgingival instrumentation and normal saline irrigation in all target sites.

Test and control intervention will be repeated for another 2 applications at an interval of 3 days. Subjects were re-examined and plaque sampling will be repeated at 6 weeks and 12 weeks.

statistical analysis: Clinical parameters were reported as mean and standard deviation. P-value <0.05 was considered statistically significant. intergroup and intragroup comparison of mean bleeding score, pocket depth, clinical attachment level will be conducted using SPSS software. For the metagenomic data, alpha and beta diversity were computed using QIIME 2. The difference in the relative abundance of individual genus over time will be analysed.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 4
• Est. completion date: April 30, 2021
• Est. primary completion date: April 30, 2021
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 21 Years and older

Eligibility

Inclusion Criteria:

• • Subject diagnosed with generalised periodontitis Stage III of any grade (Caton et al., 2018).

• Age =21 years old.

• Subject is in good general health without any remarkable past history of disease except for well controlled diabetes (HbA1c =6.5%) (Clinical practice guidelines on management of type 2 diabetes mellitus., 2020).

• Presence of at least 20 teeth.

• Presence of at least 2 non-adjacent sites located in contralateral quadrant with interproximal probing pocket depth (PPD) =6mm.

Exclusion Criteria:

• • Received subgingival scaling in the previous 6 months.

• Use of antibiotic in the past 6 months.

• Taking steroidal and non-steroidal anti-inflammatory agents on a daily basis.

• Medical condition requiring antibiotic prophylaxis.

• Pregnancy or lactating.

• Smoking =10 cigarettes per day.

Related Conditions & MeSH terms

• Periodontitis

Intervention

Drug:
• 0.85% synthetic antimicrobial peptide TAPS-18
commercially available synthetic antimicrobial peptide gel for periodontal disease treatment
• 0.9% normal saline
commercially available normal saline Sodium Chloride irrigation solution

Locations

Country	Name	City	State
Malaysia	faculty of dentistry, University of Malaya	Kuala Lumpur

Sponsors (1)

Lead Sponsor	Collaborator
University of Malaya

Country where clinical trial is conducted

Malaysia, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	clinical measurement of pocket depth (mm)	changes in pocket depth after the test and control intervention. pocket depth is measured from the gingival margin to base of pocket using UNC-15 probe	6 weeks and 12 weeks
Primary	clinical measurement of clinical attachment level (mm)	changes in clinical attachment level after the test and control intervention. clinical attachment level is measured from cemento-enamel junction to base of pocket using UNC-15 probe.	6 weeks and 12 weeks
Primary	metagenomic analysis of the relative abundance (%) of bacterial from subgingival plaque	changes in relative abundance of bacterial after the test and control intervention is measured by the metagenomic analysis using subgingival plaque	6 weeks and 12 weeks