PPAR, RXR, and VDR Expressions in Peri-implantitis

Periodontitis Clinical Trial

Official title:

PPAR-γ, RXR, and VDR Expressions in Gingival Tissue Samples of Healthy Individuals, Periodontitis and Peri-implantitis Patients

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT04020146
• Other study ID #: Gaziosmanpasa University
• Status: Completed
• Start date: January 10, 2018
• Est. completion date: March 10, 2019

Study information

• Verified date: July 2019
• Source: Tokat Gaziosmanpasa University
• Contact: n/a
• Is FDA regulated: No
• Study type: Observational

Clinical Trial Summary

ABSTRACT Objective: Periodontitis and peri-implantitis are the irreversible destructive diseases of the periodontal and peri-implant tissues. The present study aimed to determine the receptor expressions of Peroxisome proliferative-activated receptor (PPAR)-γ, Retinoid X receptor (RXR)-α, and Vitamin D receptor (VDR) in the diseased tissues around teeth and dental implants.

Methods: The study consisted of three groups as group 1; healthy controls (C, n=15), group 2; periodontitis patients with stage 3 grade B (P, n=15), and group 3; peri-implantitis patients (PI, n=15). Periodontal clinical parameters as the plaque index (PI), gingival index (GI) and clinical attachment levels (CAL) were recorded. Gingival biopsies were obtained from all participants and biopsy samples underwent histological tissue processing. Hematoxylin-eosin (H&E) and immunohistochemistry staining were performed. Total inflammatory cell counts and fibroblast cell density were evaluated on H&E-stained slides while PPAR-γ, RXR-α, and VDR were evaluated via immunohistochemistry.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 45
• Est. completion date: March 10, 2019
• Est. primary completion date: January 10, 2019
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: N/A and older

Eligibility

Inclusion Criteria:

• healthy individuals

• periodontitis patients

• periimplantitis patients

Exclusion Criteria:

• smokers,

• systemically compromised patients

Related Conditions & MeSH terms

• Peri-Implantitis
• Periimplantitis
• Periodontitis

Intervention

Other:
• Gingival biopsies
Gingival biopsies were obtained from all participants and biopsy samples underwent histological tissue processing.

Locations

Country	Name	City	State
Turkey	Gaziosmanpasa University	Tokat

Sponsors (1)

Lead Sponsor	Collaborator
Tokat Gaziosmanpasa University

Country where clinical trial is conducted

Turkey, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Inflammatory cells counts	Gingival biopsies were obtained from all participants and biopsy samples underwent histological tissue processing. Hematoxylin-eosin (H&E) staining were performed. Total inflammatory cell counts density were evaluated on H&E-stained slides.A cell counting frame of 10.000 µm2 area was selected under 1000x magnification. The total inflammatory cells (neutrophil, lymphocyte, eosinophil, and macrophage cells) within the frame were counted as inflammatory cell counting.	6 months
Primary	Fibroblast counts	Fibroblasts counts were evaluated from H&E stained slides under 1000x magnification via a light microscope (Nikon Eclipse, E 600, Tokyo, Japan).	6 months
Primary	Peroxisome proliferative-activated receptor (PPAR)-?	Gingival biopsies were obtained from all participants and biopsy samples underwent histological tissue processin and immunohistochemistry staining were performed, PPAR-?, was evaluated via immunohistochemistry. Samples were examined under 400x magnification using light microscopy (Nikon Eclipse, E 600, Tokyo, Japan).	During histological processing
Primary	Retinoid X receptor (RXR)-a	Gingival biopsies were obtained from all participants and biopsy samples underwent histological tissue processing and immunohistochemistry staining were performed, RXR-a was evaluated via immunohistochemistry. Samples were examined under 400x magnification using light microscopy (Nikon Eclipse, E 600, Tokyo, Japan).	6 months
Primary	Vitamin D receptor (VDR)	. Gingival biopsies were obtained from all participants and biopsy samples underwent histological tissue processing and immunohistochemistry staining were performed, VDR was evaluated via immunohistochemistry. Samples were examined under 400x magnification using light microscopy (Nikon Eclipse, E 600, Tokyo, Japan).	6 months