Patients Scheduled for Colonoscopy Clinical Trial
Official title:
The Real Distribution of Microbiota Along the Colonic Mucosa Using a Novel Device Capable of Taking 'Protected' Biopsies
The human microbiota forms a highly complex ecosystem with its host, consisting of hundreds
of different species of microorganisms, the majority of which have not yet been cultured.
With the recent advent of small subunit rRNA (SSU rRNA) gene sequencing technology, it is
estimated that the number of specific gastrointestinal tract phylotypes is more than 1800.
Sampling techniques might constitute a major confounder in the read-out of highly sensitive
techniques such as SSU-DNA analysis.
It is not properly established whether there is a difference in distribution of luminal
bacteria or mucosa adherent bacteria proximal or distal in the colon. In addition, 'bowel
lavage' before endoscopy might result in a disturbance of the microbiota in the bowel. For
this proof of concept study a novel device capable of taking 'protected' biopsies has been
designed.
We hypothesize that the distribution of mucosal and luminal microbiota changes from proximal
to distal in the colon, and by taking 'protected biopsies' there will be the opportunity to
show the real distribution of microbiota according to the localisation in the colon.
Furthermore, we hypothesize that microbial diversity will differ after bowel lavage.
We hypothesize that the distribution of mucosal and luminal microbiota changes from proximal to distal in the colon, and by taking 'protected biopsies' there will be the opportunity to show the real distribution of microbiota according to the localisation in the colon. ;
Observational Model: Case-Only, Time Perspective: Cross-Sectional