Diagnostic Test Validity of Structural Vertebral Endplate Defects

Defect of Vertebral Endplate (Disorder) Clinical Trial

Official title:

Structural Vertebral Endplate Defects: Detection and Characterization on Clinical-CT and Micro-CT: A Diagnostic Test Validity Study

Clinical Trial Details — Status: Active, not recruiting

Administrative data

• NCT number: NCT04808960
• Other study ID #: 01202020
• Status: Active, not recruiting
• Phase: N/A
• Start date: September 17, 2020
• Est. completion date: May 25, 2021

Study information

• Verified date: March 2021
• Source: Western University, Canada
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

Back pain is the most common cause of disability with a substantial burden on affected individuals, their families and society. Unfortunately, the causes of back pain are not well understood, hindering the development of effective prevention and treatment approaches. Until recently, the thin interface structure (endplate) between the vertebrae and the intervertebral disc has attracted attention as a possible contributor to back pain problems. However, the absence of clear descriptions for endplate defects has clouded associations with back pain and other imaging findings, which is problematic for measurement reliability. Thus, with CT, as with MRI, the varied measurement methods used to document the appearance of endplate defects on clinical imaging in the scientific literature, and the absence of validation of measurements of such methods leaves uncertainty about what the observations on clinical imaging actually represent.

Furthermore, Clinical CT is one of the most common imaging modalities used for the spine. On the other hand, Micro-CT, which produces accurate and reproducible measurements, has been used as a reference standard. The planned study will provide information that is critical for establishing meaningful standards for the evaluation and interpretation of endplate defects on clinical imaging, as well as for studies of their etiology and clinical consequences.

The study comprises 19 spines from 9 male and 10 female cadavers aged between 62 to 91 years from Western. Clinical-CT scans acquired on the cadavers will be assessed independently by two experienced imaging scientists (radiologists) and a graduate student studying endplate defects. A master's student in Clinical Anatomy has assessed and documented endplate defects on micro-CT, the reference standard. For the purpose of training and to identify practical issues in the evaluation process; four joint training sessions were organized for the raters to evaluate training sets of clinical CT images and discuss practical issues, using an evaluation manual consisting of measurement descriptions and figures. All the raters indicated satisfaction with the understanding and ability to use each measurement method. Thereafter, the three raters will independently assess the clinical CT for endplate defect presence, types, size and location. Endplate defects will be assessed with two weeks between measurements. Data will be imported into a secured computer for analysis.

Description:

Background: Back pain is a common health problem and a substantial burden on affected individuals, their families and society. In fact, it is the single leading cause of disability worldwide. Unfortunately, the pathological mechanisms behind back pain are not well understood, hindering the development of well-targeted, effective prevention and treatment approaches. The anatomical structures referred to as the functional spinal units, each composed of an intervertebral disc, its two adjacent vertebrae, and other associated osteoligamentous structures, have been studied for several decades with limited success in identifying the source of the pain. The intervertebral disc has received the most attention, and while disc degeneration or pathology is associated with the clinical conditions of disc herniation and radiculopathy, as well as spinal stenosis, the role of the disc in common back pain is uncertain. More recently, the vertebral endplate has attracted attention as a possible contributor to back pain problems.

The vertebral endplate is a thin interface structure between the intervertebral disc and the cancellous bone of the adjacent vertebral body. Unlike the disc, the bony vertebral endplate is highly vascularized and has a plentiful neural supply, and structural defects or lesions in the endplate are relatively common in cadaveric samples and on clinical imaging. However, there is a wide range of prevalence rates from 9% to 75% for structural endplate defects across studies and conflicting findings on the association of structural endplate defects with back pain. Also, there is a lack of consistency in the use of terms and definitions for endplate defects, which limits the ability to effectively communicate findings, interpret and compare study results, and build a coherent body of knowledge on the causes and consequences of endplate defects.

Furthermore, the absence of clear definitions or descriptions for endplate defects represented by various terms is problematic for measurement reliability, which is seldom reported in studies of endplate defects. A morphologic description has been found to be more important in establishing the reliability of a standardized MRI nomenclature than the experience of the reader. However, a recent review identified few measurement methods with detailed classification and definition of structural endplate phenotypes. Among them were methods presented by Feng's and Brayda-Bruno's team. Both measurement methods stem from a previous study on endplate defect phenotypes observed in a cadaveric sample. Good to excellent intra- and inter-rater reliability was reported for each classification system in a single MRI study, but neither has been validated.

Clinical CT is one of the most common imaging modalities used for the spine and its use is expected to increase with advancements in clinical CT technology, such as the low tube potential and iterative reconstruction that decreases scan time and radiation dose while optimizing image quality. Yet, with CT, as with MRI, the varied measurement methods used to document the appearance of endplate defects on clinical imaging in the scientific literature, and the absence of validation of such methods leaves uncertainty about what the observations on imaging actually represent. It is also unclear how sensitive clinical imaging is in picking up different types and sizes of defects (e.g. diagnostic accuracy). Micro-CT has been used as a reference standard in human studies and for the characterization of morphological features of endplate defects in cadaveric samples. Micro-CT measurement is accurate, consistent and reproducible, and has the advantage of providing shape and texture information for an object within a single measurement.

Our study will address the reliability and diagnostic test validity of structural endplate defect assessments of clinical CT. Such information is critical for establishing meaningful standards for the evaluation and interpretation of endplate defects on imaging, as well as for studies of their etiology and clinical consequences.

STUDY OBJECTIVES This study seeks to evaluate and compare the reliability and validity of two methods to identify and characterize structural endplate defects on clinical imaging.

The specific objectives are to:

1. Determine the inter-rater reliability of endplate defect determinations on clinical CT scans.

2. Determine the diagnostic accuracy of assessments of clinical CT-scans for identifying endplate defects, using micro-CT as the reference standard.

MATERIALS & METHODS This is a reliability and validity study of vertebral endplate defects on clinical CT (index test) using micro-CT as the reference standard in 19 human cadavers.

Materials Source: This study comprises cadavers of 9 men and 10 women with a median age of 82 years (range 62-91) from the Anatomy lab and Western University's whole-body donation program maintained by the Department of Anatomy and Cell Biology, Schulich School of Medicine and Dentistry. We will use a sample of 19 harvested T7-S1 spines from embalmed (fixed) cadavers. Embalming consisted of arterial distribution of embalming fluid, containing a mixture of ethanol, phenol, and formalin (Wessel & Associates: Troy, MI, USA), 24- to 48- hours post-mortem).

Donor information: Only the anonymized cadaver ID number, cause of death, age, and sex of the donor were available from Western's donation program.

Spine imaging Clinical CT was acquired (scanned at 1.25mm x 1.25mm on a standard algorithm and then reformatted into an axial, coronal and sagittal series at 3mm x 2mm) on all fully intact cadavers prior to the onset of this study by the Schulich School of Medicine & Dentistry at the University of Western Ontario, in accordance with the Anatomy Act of Ontario and guidelines of Western's Committee for Cadaveric Use in Research.

Micro-CT (µCT) of all cadaveric specimens was performed at Robarts Research Institute at a peak voltage of 80 kVp and a tube current of 50 mA. The X-ray projections were reconstructed into a single three-dimensional volume with isotropic voxel spacing of 154 µm. In preparation for µCT the lower thoracic and lumbar spine (T7 through S1) was removed from each cadaver. The ribs were dissected 1-2 cm lateral to the costovertebral joints and the soft tissue associated with the spine was preserved and remained intact during micro-CT.

Assessment of endplate defects Assessment of clinical CT scans: Three raters will independently assess the sagittal images of the clinical CT for endplate defect presence, phenotype classification, and defect size and location, blinded to all prior assessments. Endplate defect measurement methods by Brayda-Bruno's and Feng's teams will be strictly used. First, measurement according to Brayda-Bruno will be used to assess the 19 clinical CTs of the spines. Two weeks after the Brayda-Bruno assessment, evaluations according to Feng will be performed on sagittal images to determine the type and location of the defects, as well as to measure the anteroposterior and transverse diameter of the defects and endplates. Across all measurements, endplates will be labelled with reference to the disc. All endplate defect measurements will be observed from the immediate normal slice before the defect to the first normal slice after the defect. Feng's initial measurement protocol used only sagittal MR images to estimate the transverse diameter by dividing the number of images with endplate defects by the total number of sagittal images containing the endplate. However, due to the large number of sagittal images in CT compared to MRI, the current study will use the transitional distance covered by the defects in relation to the image to determine the transverse diameter. Artefact including osteophytes will be excluded when taking an endplate measurement. Each rater will assess the images in the same order (Brayda-Bruno's followed by Feng's measurements). All measurements will be taken in millimetres rounded up to the nearest decimal point. All assessments will be entered into a pilot-tested excel data entry form. After all assessments, a panellist with the three raters will discuss each rating for a consensus on the single index test that will be compared to the reference standard.

Raters, training, and Consensus The clinical-CT scans will be assessed independently by two radiologists with experience in musculoskeletal imaging and a physical therapist, a Ph.D. student studying endplate defects. A master's student in Clinical Anatomy has assessed and documented endplate defects on micro-CT, the reference standard.

To ensure a consistent understanding of the nomenclature in each classification system, an evaluation manual consisting of measurement descriptions and figures was prepared based on the published articles introducing each of the classification systems. For the purpose of training and to identify practical issues in the evaluation process; four joint training sessions were organized for the raters to evaluate training sets of clinical CT images and discuss practical issues. Each rater independently rated three clinical CT images (66 endplates). Results were collected and analyzed, conflicting domains were noted, discussed and clarified during meetings. All the raters indicated satisfaction with the understanding and ability to use each measurement method.

Statistical Analysis Data will be imported into Stata/IC 14 (Stata Corp LP) for statistical analysis. Inter-rater reliability among the three raters (objective 1) of the clinical CT measurements will be determined using Cohen's kappa statistic for categorical variables (presence or absence of defect and the type of the defect) or Intraclass Correlation Coefficients (ICC) for continuous variables (e.g., anteroposterior and transverse diameters.). ICC and Kappa values will be interpreted as poor (< 0.40), fair (0.41-0.60), good (0.61-0.80), and excellent (0.81-1.0). Discrepancies will then be reviewed and resolved through consensus. For objective 2, for each endplate level, a 2x2 table will be used to calculate percent agreement in detecting endplate defects between the clinical CT and micro-CT.

Recruitment information / eligibility

• Status: Active, not recruiting
• Enrollment: 19
• Est. completion date: May 25, 2021
• Est. primary completion date: May 25, 2021
• Accepts healthy volunteers: No
• Gender: All
• Age group: 62 Years to 91 Years

Eligibility

Inclusion Criteria: All cadavers available for the harvesting of spinal segments T7-S1 with endplates and vertebral bodies intact and undamaged by prior use in the Anatomy Lab will be used.

-

Exclusion Criteria: There were no other exclusions.

-

Related Conditions & MeSH terms

• Defect of Vertebral Endplate (Disorder)

Intervention

Diagnostic Test:
• clinical Computed Tomography
Clinical CT was acquired (scanned at 1.25mm x 1.25mm on a standard algorithm and then reformatted into an axial, coronal and sagittal series at 3mm x 2mm) on all fully intact cadavers prior to the onset of this study by the Schulich School of Medicine & Dentistry at the University of Western Ontario, in accordance with the Anatomy Act of Ontario and guidelines of Western's Committee for Cadaveric Use in Research.

Locations

Country	Name	City	State
Canada	University of Western Ontario	London	Ontario

Sponsors (2)

Lead Sponsor	Collaborator
Western University, Canada	London Health Sciences Centre

Country where clinical trial is conducted

Canada, 

References & Publications (9)

Battié MC, Videman T, Kaprio J, Gibbons LE, Gill K, Manninen H, Saarela J, Peltonen L. The Twin Spine Study: contributions to a changing view of disc degeneration. Spine J. 2009 Jan-Feb;9(1):47-59. doi: 10.1016/j.spinee.2008.11.011. — View Citation

Brant-Zawadzki MN, Jensen MC, Obuchowski N, Ross JS, Modic MT. Interobserver and intraobserver variability in interpretation of lumbar disc abnormalities. A comparison of two nomenclatures. Spine (Phila Pa 1976). 1995 Jun 1;20(11):1257-63; discussion 1264. — View Citation

Brayda-Bruno M, Albano D, Cannella G, Galbusera F, Zerbi A. Endplate lesions in the lumbar spine: a novel MRI-based classification scheme and epidemiology in low back pain patients. Eur Spine J. 2018 Nov;27(11):2854-2861. doi: 10.1007/s00586-018-5787-6. Epub 2018 Oct 10. — View Citation

Feng Z, Liu Y, Yang G, Battié MC, Wang Y. Lumbar Vertebral Endplate Defects on Magnetic Resonance Images: Classification, Distribution Patterns, and Associations with Modic Changes and Disc Degeneration. Spine (Phila Pa 1976). 2018 Jul 1;43(13):919-927. doi: 10.1097/BRS.0000000000002450. — View Citation

Lawan A, Leung A, Battié MC. Vertebral endplate defects: nomenclature, classification and measurement methods: a scoping review. Eur Spine J. 2020 Jun;29(6):1397-1409. doi: 10.1007/s00586-020-06378-8. Epub 2020 Mar 26. Review. — View Citation

Wang Y, Battié MC, Boyd SK, Videman T. The osseous endplates in lumbar vertebrae: thickness, bone mineral density and their associations with age and disk degeneration. Bone. 2011 Apr 1;48(4):804-9. doi: 10.1016/j.bone.2010.12.005. Epub 2010 Dec 17. — View Citation

Wang Y, Videman T, Battié MC. ISSLS prize winner: Lumbar vertebral endplate lesions: associations with disc degeneration and back pain history. Spine (Phila Pa 1976). 2012 Aug 1;37(17):1490-6. doi: 10.1097/BRS.0b013e3182608ac4. — View Citation

Wang Y, Videman T, Battié MC. Modic changes: prevalence, distribution patterns, and association with age in white men. Spine J. 2012 May;12(5):411-6. doi: 10.1016/j.spinee.2012.03.026. Epub 2012 Apr 18. — View Citation

Zehra U, Cheung JPY, Bow C, Lu W, Samartzis D. Multidimensional vertebral endplate defects are associated with disc degeneration, modic changes, facet joint abnormalities, and pain. J Orthop Res. 2019 May;37(5):1080-1089. doi: 10.1002/jor.24195. Epub 2019 Mar 28. — View Citation

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Vertebral structural endplate defect phenotype (Brayda-Bruno scale)	Presence of endplate defects phenotypes assessed according to Brayda-Bruno, including wavy/irregular, notched, Schmorl's node and fracture which are recorded mutually exclusive	Baseline
Primary	Vertebral structural endplate defect phenotype (Feng Sclae)	Presence of endplate defects phenotypes assessed according to Feng, including focal, corner and erosion which are recorded mutually exclusive	Baseline
Secondary	Vertebral structural endplate defect dimensions	Dimensions of endplate defects phenotypes assessed according to Feng, including Antero-posterior, and transverse diameter of the defects will be assessed and measured in millimetres.	Baseline
Secondary	Axial area of Vertebral structural endplate defects	Axial area of the endplate defect assessed according to Feng, which depend on the location of the defect; area will be rated on an ordinal scale as none if no defects are present, small if one or two sections are involved, moderate if three sections are involved or large if four or five sections are involved.	Baseline