Substrate Metabolism During Exercise Following Ingestion of Ethanol

Exercise Metabolism Following Ethanol Ingestion Clinical Trial

Official title:

Substrate Metabolism During 2 Hours of Moderate Intensity Exercise Following Ingestion of Ethanol in Man

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT03404947
• Other study ID #: ETOH-hs565
• Status: Completed
• Phase: N/A
• Start date: November 13, 2017
• Est. completion date: June 29, 2018

Study information

• Verified date: March 2019
• Source: University of Bath
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

This study will investigate how ethanol (pure alcohol) influences carbohydrate and fat metabolism during prolonged, moderate intensity exercise. Participants will perform two bouts of cycling exercise with or without prior ingestion of ethanol, in a randomised order, separated by one week.

Description:

Very little research has investigated the influence of ethanol on metabolism during prolonged exercise. Evidence suggests that delivery of fat to the working muscles during exercise may be limited with ethanol and therefore this may shift the relative proportions of energy derived from muscle stores of carbohydrate as a result (Jorfeldt & Juhlin-Dannfelt, 1976).

The current study aims to investigate how a small-moderate dose of ethanol influences where energy is derived from during a prolonged bout of moderate intensity cycling exercise.

Participants will visit the lab on three occasions, once for preliminary measurements of fitness and body composition, and twice for the experimental bouts of exercise. Body composition will be assessed using a dual-energy x-ray absorptiometry (DEXA) scan, which will measure lean mass, fat mass, and bone mineral density. Participants will then perform an incremental maximal oxygen uptake test to determine intensity for the experimental bouts of exercise.

The two experimental bouts of exercise include cycling on an exercise bike for 2 hours at 55% of their maximal oxygen uptake (i.e. fitness) following 1 hours rest. This will be performed under two conditions: with and without ethanol ingestion. Pre and post exercise muscle biopsies will be collected to assess muscle metabolism, with regular blood samples and expired breath samples being collected to further investigate fuel delivery and use by the working tissues. The experimental bouts of exercise will be performed 1 week apart in a randomised order.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 8
• Est. completion date: June 29, 2018
• Est. primary completion date: June 29, 2018
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Male
• Age group: 18 Years to 30 Years

Eligibility

Inclusion Criteria:

• Habitual alcohol consumer (average 1-14 units per week)

• BMI 18.5 - 29.9 kg/m2

• Be able to attend the laboratory and willing to participate in necessary protocols

• Be willing to undertake the durations of the exercise protocol (2 hours) ]

• Have the capacity and willingness to provide informed consent (oral and written)

Exclusion Criteria:

• Non-or excessive alcohol drinkers (0 or >14 units per week)

• History of substance abuse

• Habitual/previously habitual smokers

• BMI >30.0 kg/m2

• Experience contraindications to the administration of the proposed anaesthetic (Lidocaine Hydrochloride)

Related Conditions & MeSH terms

• Exercise Metabolism Following Ethanol Ingestion

Intervention

Dietary Supplement:
• Ethanol
For one exercise session, participants will be asked to ingest a quantity of ethanol (in the form of vodka) sufficient to maximally stimulate liver alcohol metabolism.

Locations

Country	Name	City	State
United Kingdom	University of Bath	Bath	Somerset

Sponsors (1)

Lead Sponsor	Collaborator
University of Bath

Country where clinical trial is conducted

United Kingdom, 

References & Publications (3)

Jorfeldt L, Juhlin-Dannfelt A. The influence of ethanol on human splanchnic and skeletal muscle metabolism during exercise. Scand J Clin Lab Invest. 1977 Nov;37(7):609-18. — View Citation

Juhlin-Dannfelt A, Ahlborg G, Hagenfeldt L, Jorfeldt L, Felig P. Influence of ethanol on splanchnic and skeletal muscle substrate turnover during prolonged exercise in man. Am J Physiol. 1977 Sep;233(3):E195-202. — View Citation

Juhlin-Dannfelt A, Jorfeldt L, Hagenfeldt L, Hulten B. Influence of ethanol on non-esterified fatty acid and carbohydrate metabolism during exercise in man. Clin Sci Mol Med. 1977 Sep;53(3):205-14. — View Citation

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Change in muscle glycogen content	Muscle biopsy and subsequent biochemical analysis	Change in muscle glycogen content over 2 hours of moderate intensity cycling exercise
Primary	Blood glucose concentration	Venous blood samples will be taken and biochemically analysed for blood glucose concentration.	Blood samples will be taken every 15 minutes during rest and the first hour of exercise, they will be taken every 30 minutes in the second hour of exercise.
Primary	Blood non-esterified fatty acid (NEFA) concentration	Venous blood samples will be taken and biochemically analysed for NEFA concentration.	Blood samples will be taken every 15 minutes during rest and the first hour of exercise, they will be taken every 30 minutes in the second hour of exercise.
Secondary	Blood ethanol concentration	Venous blood samples will be taken and biochemically analysed for blood ethanol concentration.	Blood samples will be taken every 15 minutes during rest and the first hour of exercise, they will be taken every 30 minutes in the second hour of exercise.
Secondary	Blood lactate concentration	Venous blood samples will be taken and biochemically analysed for blood lactate concentration.	Blood samples will be taken every 15 minutes during rest and the first hour of exercise, they will be taken every 30 minutes in the second hour of exercise.
Secondary	Carbohydrate Oxidation	Carbohydrate oxidation will be determined through indirect calorimetry via the douglas bag technique.	Expired breath samples will be collected at baseline before beverage consumption and again after 1 hours rest. Samples will collected every 15 minutes during the first hour of exercise and every 30 minutes during the second hour
Secondary	Fat Oxidation	Fat oxidation will be determined through indirect calorimetry via the douglas bag technique.	Expired breath samples will be collected at baseline before beverage consumption and again after 1 hours rest. Samples will collected every 15 minutes during the first hour of exercise and every 30 minutes during the second hour